Evaluation of a Latex Agglutination Method for Detecting and Characterizing Verotoxin (VT) Produced by Escherichia coli

Kai, Akemi; Obata, Hiromi; Hatakeyama, Kaoru; Igarashi, Hideo; Itoh, Takeshi; Kudoh, Yasuo

doi:10.11150/kansenshogakuzasshi1970.71.248

Cited by 10 publications

(3 citation statements)

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“…The supernatant fraction was used for the measurement of verotoxin. Verotoxin production was measured by latex agglutination reaction using the ‘SEIKEN’ VTEC‐RPLA (Denka Seiken Co. Ltd, Japan) which was developed to detect verotoxin ( Kai et al . 1997 ).…”

Section: Methodsmentioning

confidence: 99%

Inhibitory effect of creosote and its main components on production of verotoxin of enterohaemorrhagic Escherichia coli O157:H7

Sakagami

Ichise²,

Kajimura

et al. 1999

Letters in Applied Microbiology

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The inhibitory effects of creosote and its main components, α‐methoxyphenol and o‐ethylphenol, on production of verotoxin by enterohaemorrhagic Escherichia coli O157 (VTEC E. coli) were investigated. The production of verotoxin by VTEC E. coli was inhibited by the administration of 0·001≈0·1% of creosote or o‐ethylphenol (final concentration). On the other hand, weak inhibition of production of verotoxin was observed with 0·1%α‐methoxyphenol administration. As the inhibitory effects were obtained below Minimal Inhibitory Concentration (MIC) values, these test compounds exerted their effects at the active site of VTEC E. coli cells prior to their production of verotoxin. These findings suggest that pre‐administration of creosote and its main components might prevent human intestinal food poisoning by VTEC E. coli and contribute to maintenance of public health.

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Section: Methodsmentioning

confidence: 99%

Inhibitory effect of creosote and its main components on production of verotoxin of enterohaemorrhagic Escherichia coli O157:H7

Sakagami

Ichise²,

Kajimura

et al. 1999

Letters in Applied Microbiology

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“…58,[62][63][64][65] This commercial test kit uses reversed passive latex agglutination to detect shiga-like toxins. The reference test used was verocell cytoxicity, and in some cases additional stx gene-specific PCR.…”

Section: Vtec Screen (Denka Seiken)mentioning

confidence: 99%

A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food

Abubakar¹,

Irvine²,

Aldus³

et al. 2007

Health Technol Assess

103

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“…The resulting supernatants were collected and freeze-dried. Samples were analyzed for verotoxin content by RPLA with a semiquantitative commercial kit (VTEC-RPLA, Seiken, Japan) (18). An experiment was conducted to determine the effect of plant extract on toxin that could alter quantification by RPLA.…”

Section: Effect Of the Extracts On Verotoxin Productionmentioning

confidence: 99%

Extracts of Haematoxylon brasiletto Inhibit Growth, Verotoxin Production, and Adhesion of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells

Heredia

Escobar

Rodríguez‐Padilla

et al. 2005

Journal of Food Protection

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The extracts of 33 plants were evaluated for their effects on growth of Escherichia coli O157:H7 (EHEC). The extract of Haematoxylon brasiletto was the only one that effectively inhibited bacterial growth. The effects of ethanolic extracts of this plant on growth, verotoxin production, and adhesion of E. coli O157:H7 to HeLa cells were determined. The MBC for growth was 4 mg/ml. No verotoxin formation was detected at 1, 2, or 3 mg/ml. Preexposing bacteria and HeLa cells to various concentrations of extracts affected the adhesion between non-EHEC and HeLa cells. Partial purification of the active fraction suggested that polyphenols might play a role in the antimicrobial activity exhibited by H. brasiletto extracts.

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Evaluation of a Latex Agglutination Method for Detecting and Characterizing Verotoxin (VT) Produced by Escherichia coli

Cited by 10 publications

References 0 publications

Inhibitory effect of creosote and its main components on production of verotoxin of enterohaemorrhagic Escherichia coli O157:H7

Inhibitory effect of creosote and its main components on production of verotoxin of enterohaemorrhagic Escherichia coli O157:H7

A systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food

Extracts of Haematoxylon brasiletto Inhibit Growth, Verotoxin Production, and Adhesion of Enterohemorrhagic Escherichia coli O157:H7 to HeLa Cells

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