Evaluation of a model for the effects of substrate interactions on the kinetics of reductive dehalogenation

Wrenn, Brian A.; Rittmann, Bruce E.

doi:10.1007/bf00056558

Cited by 13 publications

(9 citation statements)

References 52 publications

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“…Dehalobacter strain TCA1 could completely dechlorinate 450 M (60 mg/liter) 1,1,1-TCA to CA in 5 weeks (45); no degradation characteristics have been reported for the mixed culture from which this isolate was obtained. Other studies (8,42,43,54) have reported the degradation of significant amounts of 1,1,1-TCA, but these concentrations were less than those tested in the present study. Therefore, while the current study represents the highest reported 1,1,1-TCA concentration degraded, little data exist for comparison.…”

Section: Discussioncontrasting

confidence: 73%

A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes

Grostern¹,

Edwards

2006

Appl Environ Microbiol

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-TCA) is a common groundwater pollutant as a result of improper disposal and accidental spills. It is often found as a cocontaminant with trichloroethene (TCE) and inhibits some TCEdegrading microorganisms. 1,1,1-TCA removal is therefore required for effective bioremediation of sites contaminated with mixed chlorinated organics. This study characterized MS, a 1,1,1-TCA-degrading, anaerobic, mixed microbial culture derived from a 1,1,1-TCA-contaminated site in the northeastern United States. MS reductively dechlorinated 1,1,1-TCA to 1,1-dichloroethane (1,1-DCA) and then to monochloroethane (CA) but not further. Cloning of bacterial 16S rRNA genes revealed among other organisms the presence of a Dehalobacter sp. and a Desulfovibrio sp., which are both phylogenetically related to known dehalorespiring strains. Monitoring of these populations with species-specific quantitative PCR during degradation of 1,1,1-TCA and 1,1-DCA showed that Dehalobacter proliferated during dechlorination. Dehalobacter growth was dechlorination dependent, whereas Desulfovibrio growth was dechlorination independent. Experiments were also performed to test whether MS could enhance TCE degradation in the presence of inhibiting levels of 1,1,1-TCA. Dechlorination of cis-dichloroethene (cDCE) and vinyl chloride (VC) in KB-1, a chloroethenedegrading culture used for bioaugmentation, was inhibited with 1,1,1-TCA present. When KB-1 and MS were coinoculated, degradation of cDCE and VC to ethene proceeded as soon as the 1,1,1-TCA was dechlorinated to 1,1-DCA by MS. This demonstrated the potential application of the MS and KB-1 cultures for cobioaugmentation of sites cocontaminated with 1,1,1-TCA and TCE.

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Section: Discussioncontrasting

confidence: 73%

A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes

Grostern¹,

Edwards

2006

Appl Environ Microbiol

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“…This paper describes the complete biological cometabolic transformation of TCA to CA and the conditions necessary for this complete transformation. In general, the rate of cometabolic dechlorination processes increases with an increase in electron donor concentration (Doong and Wu 1996;Wrenn and Rittmann 1996). Here we report that, not only the rate of dechlorination, but also the nature of the products depend on the electron donor concentration.…”

Section: Discussionmentioning

confidence: 55%

“…more TCA was transformed to CA than with methanol as an electron donor. The eect of dierent electron donors on the products of biological dechlorination has never been described, but there are several reports of the eect of dierent electron donors on (the rate of) microbial dechlorination (Bagley and Gossett 1990;Holliger 1992;Lewis and Crawford 1993;Petrovskis et al 1994;Wrenn and Rittmann 1996).…”

Section: Discussionmentioning

confidence: 99%

Complete transformation of 1,1,1-trichloroethane to chloroethane by a methanogenic mixed population

Best¹,

Hage²,

Doddema³

et al. 1999

Applied Microbiology and Biotechnology

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Complete transformation of 1,1,1-trichloroethane to chloroethane by a methanogenic mixed population de Best, JH; Hage, A; Doddema, HJ; Janssen, DB; Harder, W Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract A methanogenic mixed population in a packed-bed reactor completely transformed 1,1,1-trichloroethane (10 lM) to chloroethane by a cometabolic process. Chloroethane was not further transformed. Acetate and methanol served as electron donors. Complete transformation of 1,1,1-trichloroethane to chloroethane only occurred when sucient electron donor was fed into the reactor. Otherwise, besides chloroethane, 1,1-dichloroethane was also found as a product. The products of 1,1,1-trichloroethane transformation also depended on the type of electron donor present. With acetate, the degree of dechlorination was higher, i.e. more 1,1,1-trichloroethane was transformed to chloroethane than with methanol. In an enrichment culture obtained from the reactor contents, 1,1,1-trichloroethane was only transformed to 1,1-dichloroethane and was not further metabolized. Methanol, acetate, formate, ethanol, 2-propanol, trimethylamine and H 2 , but not dimethylamine and methylamine, served as electron donors for 1,1,1-trichloroethane transformation by this enrichment culture. Both nitrate and nitrite inhibited 1,1,1-trichloroethane transformation; while nitrate completely inhibited 1,1,1-trichloroethane dechlorination, some conversion did occur in the presence of nitrite. The product(s) of this conversion remain unknown, since no chlorinated hydrocarbons were detected.

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“…Under these conditions, no inhibition of CH 3 CCl 3 transformation was observed in our packed-bed reactor, since methanogenesis was not suppressed. Wrenn and Rittmann (1996) also reported no e ect of sulfate on CH 3 CCl 3 transformation in a methanogenic bio®lm reactor even at a sulfate/formate ratio of 1. However, in these experiments no time for adaptive changes in the microbial population was allowed.…”

Section: Discussionmentioning

confidence: 96%

“…Only Oldenhuis et al (1989) have reported the partial conversion of CH 3 CCl 3 to trichloroethanol by Methylosinus trichosporium OB3b under aerobic conditions. Transformation of CH 3 CCl 3 in anaerobic continuousow systems has been reported under both sulfatereducing and methanogenic conditions (Bouwer and McCarty 1983;Bouwer and Wright 1988;Cobb and Bouwer 1991;Vogel and McCarty 1987a;Wrenn and Rittmann 1996). Transformation only occurred after a long acclimatization period (10±12 weeks) and was only investigated at CH 3 CCl 3 concentrations below 1 lM.…”

mentioning

confidence: 99%

Transformation of 1,1,1-trichloroethane in an anaerobic packed-bed reactor at various concentrations of 1,1,1-trichloroethane, acetate and sulfate

deBest

Jongema

Weijling

et al. 1997

Applied Microbiology and Biotechnology

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Transformation of 1,1,1-trichloroethane in an anaerobic packed-bed reactor at various concentrations of 1,1,1-trichloroethane, acetate and sulfate deBest, JH; Jongema, H; Weijling, A; Doddema, HJ; Janssen, DB; Harder, W Copyright Other than for strictly personal use, it is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license (like Creative Commons).Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract Biotransformation of 1,1,1-trichloroethane (CH 3 CCl 3 ) was observed in an anaerobic packed-bed reactor under conditions of both sulfate reduction and methanogenesis. Acetate (1 mM) served as an electron donor. CH 3 CCl 3 was completely converted up to the highest investigated concentration of 10 lM. 1,1-Dichloroethane and chloroethane were found to be the main transformation products. A fraction of the CH 3 CCl 3 was completely dechlorinated via an unknown pathway. The rate of transformation and the transformation products formed depended on the concentrations of CH 3 CCl 3 , acetate and sulfate. With an increase in sulfate and CH 3 CCl 3 concentrations and a decrease in acetate concentration, the degree of CH 3 CCl 3 dechlorination decreased. Both packed-bed reactor studies and batch experiments with bromoethanesulfonic acid, an inhibitor of methanogenesis, demonstrated the involvement of methanogens in CH 3 CCl 3 transformation. Batch experiments with molybdate showed that sulfatereducing bacteria in the packed-bed reactor were also able to transform CH 3 CCl 3 . However, packed-bed reactor experiments indicated that sulfate reducers only had a minor contribution to the overall transformation in the packed-bed reactor.

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Evaluation of a model for the effects of substrate interactions on the kinetics of reductive dehalogenation

Cited by 13 publications

References 52 publications

A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes

A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes

Complete transformation of 1,1,1-trichloroethane to chloroethane by a methanogenic mixed population

Transformation of 1,1,1-trichloroethane in an anaerobic packed-bed reactor at various concentrations of 1,1,1-trichloroethane, acetate and sulfate

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