Evaluation of a modified commercial assay in detecting antibody to hepatitis C virus in oral fluids and dried blood spots

Judd, Ali; Parry, John; Hickman, Matthew; McDonald, Tamara; Jordan, Laura; Lewis, Kim; Contreras, Marcela; Dusheiko, G.; Foster, Graham R.; Gill, Noël; Kemp, Katie; Main, Janice; Murray‐Lyon, Iain M.; Nelson, Mark

doi:10.1002/jmv.10463

Cited by 151 publications

(139 citation statements)

References 18 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…17,18,21 Of the 272 blood samples, 260 (96%) were suitable for serological analysis, so this diagnostic method proved logistically feasible in primary care, as has previously been described for a variety of infectious diseases, including measles, toxoplasmosis, hepatitis C, and human immunodefi ciency virus infection. 16,[22][23][24] Although our study shows that the value of dried blood spot for serological verifi cation of herpes zoster is limited, the logistic advantages of this method, such as ease of collection, transport, and storage, are evident.Some potential limitations of this study need to be addressed to appreciate the results. First, our aim was to quantify the extent to which a clinical diagnosis of herpes zoster made by the family physician could be confi rmed serologically.…”

mentioning

confidence: 99%

Clinical Diagnosis of Herpes Zoster in Family Practice

Opstelten

Loon²,

Schuller³

et al. 2007

The Annals of Family Medicine

View full text Add to dashboard Cite

PURPOSE Family physicians usually diagnose herpes zoster on clinical grounds only, possibly resulting in false-positive diagnoses and unnecessary treatment. We wanted to determine the positive predictive value of the physicians' judgment in diagnosing herpes zoster and to assess the applicability of dried blood spot analysis for diagnosis of herpes zoster in family practice.METHODS Our study population consisted of 272 patients older than 50 years with herpes zoster (rash for less than 7 days). Dried blood spot samples were collected from all patients and sent by mail to the laboratory. Baseline measurements included clinical signs (localization, severity, and duration of rash) and symptoms (duration and severity of pain). Varicella-zoster virus antibodies were determined at baseline and 5 to 10 days later. Multivariate logistic regression was used to assess independent associations between clinical variables and serological confi rmation of herpes zoster.RESULTS Dried blood spot analysis was possible in 260 patients (96%). In 236 the diagnosis of herpes zoster was confi rmed serologically (positive predictive value of clinical judgment 90.8%; 95% confi dence interval, 87.3%-94.3%). Independent clinical variables for serologically confi rmed herpes zoster were severity and duration of rash at fi rst examination.CONCLUSION Family physicians have good clinical judgment when diagnosing herpes zoster in older patients. Dried blood spot analysis is a logistically convenient method for serological investigation of patients in family practice, but it is rarely needed for diagnosing herpes zoster. INTRODUCTIONH erpes zoster is a common disease, with a reported incidence varying from 2.2 to 4.8 per 1,000 persons per year. [1][2][3] It is due to a localized recrudescence of the varicella-zoster virus in sensory ganglia, where the virus has remained dormant since the primary infection (chickenpox). Age and immunity-attenuating diseases are well-known risk factors for herpes zoster. 4 The most frequent complications of herpes zoster include postherpetic neuralgia and, in cases of ophthalmic herpes zoster, sight-threatening eye problems.Because the typical unilateral rash helps family physicians diagnose herpes zoster clinically, suspected cases of herpes zoster are rarely investigated serologically or virologically. False-positive diagnosis of herpes zoster, however, is reported to occur in up to 13% of patients 5,6 and may result in unnecessary prescription of antiviral medications, erroneous referral, and unnecessary invasive interventions for the prevention of postherpetic neuralgia. 7 Serological analysis is one method to confi rm the diagnosis of herpes zoster, 8 but few studies have assessed its value in family practice. Moreover, such analysis in primary care can be fraught with logistic problems. In remote areas laboratory facilities may not be accessed easily, and for research purposes uniform analysis techniques at a central location may 306CLINIC A L DIAGNOSIS OF HER PES ZOS T ER be preferred over analy...

show abstract

mentioning

confidence: 99%

Clinical Diagnosis of Herpes Zoster in Family Practice

Opstelten

Loon²,

Schuller³

et al. 2007

The Annals of Family Medicine

View full text Add to dashboard Cite

show abstract

“…7% and a specificity of 99 . 2 % [19]. The observed prevalencep p d, t, a, s is related to the true prevalence p d,t,a,s as described in Sweeting et al [3].…”

Section: Hcv Prevalence Modelmentioning

confidence: 84%

“…Respondents voluntarily and anonymously provide an oral fluid sample for testing [19], and complete a brief questionnaire. The survey received multi-centre ethics committee approval.…”

Section: Data Sourcesmentioning

confidence: 99%

Spatial mapping of hepatitis C prevalence in recent injecting drug users in contact with services

Harris¹,

Hope

Morongiu³

et al. 2011

Epidemiol. Infect.

View full text Add to dashboard Cite

SUMMARYIn developed countries the majority of hepatitis C virus (HCV) infections occur in injecting drug users (IDUs) with prevalence in IDUs often high, but with wide geographical differences within countries. Estimates of local prevalence are needed for planning services for IDUs, but it is not practical to conduct HCV seroprevalence surveys in all areas. In this study survey data from IDUs attending specialist services were collected in 52/149 sites in England between 2006 and 2008. Spatially correlated random-effects models were used to estimate HCV prevalence for all sites, using auxiliary data to aid prediction. Estimates ranged from 14 % to 82 %, with larger cities, London and the North West having the highest HCV prevalence. The methods used generated robust estimates for each area, with a well-identified spatial pattern that improved predictions. Such models may be of use in other areas of study where surveillance data are sparse.

show abstract

“…In addition, it enables the shipping of samples by regular mail with no particular risk of contamination. This represents a valuable asset for sampling in remote communities either located far away from a testing laboratory or with limited technical infrastructure available, therefore provides added value compared to standard blood sampling [59]. Through its small size and stacking capacity, DBS is also a valuable solution for reducing and facilitating storage in clinical laboratories and biobanks [109].…”

Section: Pros and Cons Of Dbsmentioning

confidence: 99%

“…In addition, this analyte remains stable for over 15 days on DBS [30]. DBS is also well adapted for the enzyme-linked immunosorbent assay (ELISA) detection of specific antibodies against Epstein-Barr virus [50], Rubella virus [74], dengue virus [75] or hepatitis C [7,59] and HIV virus [13].…”

Section: Peptides -Proteinsmentioning

confidence: 99%

Current and future use of “dried blood spot” analyses in clinical chemistry

Lehmann¹,

Delaby

Vialaret

et al. 2013

Clinical Chemistry and Laboratory Medicine (CCLM)

120

View full text Add to dashboard Cite

Abstract:The analysis of blood spotted and dried on a matrix (i.e., "dried blood spot" or DBS) has been used since the 1960s in clinical chemistry; mostly for neonatal screening. Since then, many clinical analytes, including nucleic acids, small molecules and lipids, have been successfully measured using DBS. Although this preanalytical approach represents an interesting alternative to classical venous blood sampling, its routine use is limited. Here, we review the application of DBS technology in clinical chemistry, and evaluate its future role supported by new analytical methods such as mass spectrometry.

show abstract

Evaluation of a modified commercial assay in detecting antibody to hepatitis C virus in oral fluids and dried blood spots

Cited by 151 publications

References 18 publications

Clinical Diagnosis of Herpes Zoster in Family Practice

Clinical Diagnosis of Herpes Zoster in Family Practice

Spatial mapping of hepatitis C prevalence in recent injecting drug users in contact with services

Current and future use of “dried blood spot” analyses in clinical chemistry

Contact Info

Product

Resources

About