2017
DOI: 10.1371/journal.pone.0174908
|View full text |Cite
|
Sign up to set email alerts
|

Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

Abstract: Carbapenem-resistant Enterobacteriaceae (CRE) have spread globally and represent a serious and growing threat to public health. The introduction of rapid and sensitive methods for the detection of carbapenemase-producing bacteria is of increasing importance. The carbapenemase production can be detected using non-molecular methods (such as the modified Hodge test, the synergy test, the Carba NP test and the antibiotic hydrolysis assays) and DNA-based methods. In this study, we propose a modified version of a pr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

1
19
0

Year Published

2018
2018
2024
2024

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 16 publications
(20 citation statements)
references
References 21 publications
1
19
0
Order By: Relevance
“…However, this situation was significantly improved through an extension of the 1 h of incubation, with 100% sensitivity and specificity. These findings both accorded and contradicted those of the study by Calderaro et al, in which a complete hydrolysis of MEM was observed after 30 min for the KPC-producing strains and after 1 h for the class B carbapenemaseproducing strains (1). Nevertheless, our results showed that 2 h of incubation is sufficient for the detection of all class A and class B carbapenemases.…”
Section: Discussionsupporting
confidence: 72%
See 1 more Smart Citation
“…However, this situation was significantly improved through an extension of the 1 h of incubation, with 100% sensitivity and specificity. These findings both accorded and contradicted those of the study by Calderaro et al, in which a complete hydrolysis of MEM was observed after 30 min for the KPC-producing strains and after 1 h for the class B carbapenemaseproducing strains (1). Nevertheless, our results showed that 2 h of incubation is sufficient for the detection of all class A and class B carbapenemases.…”
Section: Discussionsupporting
confidence: 72%
“…As for the concentration of meropenem and the amount of organism used, previous studies had used different combinations: final quantities of meropenem ranging from 0.1 mM (almost the same quantity as our study) to 10 mM (1, 7) and bacterial loads ranging from 1 (the same as our study) to 10 l loopfuls (31, 32) or 3 to 8 McFarland (1, 2, 33), but none of the studies had evaluated the effect of both factors at the same time. We chose MEM as the substrate to incubate with bacteria based on previous suggestions that MEM is more efficient against Enterobacteriaceae (1,2). We also compared the efficiencies of 1 h and 2 h of incubation in the detection of carbapenemase activity.…”
Section: Discussionmentioning
confidence: 99%
“…Taking into account that cultivation in xenic medium is a fundamental step in parasitic diagnosis in order to reveal the presence of different parasites, the advantages of MALDI-TOF MS are evident, particularly in terms of rapidity, simplicity and cost saving. Despite the high instrument cost, the cost saving is achieved as its use is not limited to the diagnosis of dientamoebiasis alone; in fact, the use of MALDI-TOF MS is constantly increasing in the microbiology laboratories for identification of other microbial strains and so the cost would be spread across a variety of activities [ 25 ].…”
Section: Discussionmentioning
confidence: 99%
“…The imipenem assay achieved a higher sensitivity (97%) and specificity (100%) for the testing of P. aeruginosa (250 strains tested), whereas the meropenem assay achieved a higher sensitivity (99%) and specificity (100%) for Enterobacteriaceae (124 strains tested) (Rotova et al, 2017 ). In 2017 another group published results for a slightly modified meropenem assay testing 1185 enterobacterial strains from Italy, which carried mainly KPC or VIM enzymes (Calderaro et al, 2017 ). It showed that the integrity of meropenem is an important factor in the analysis of the read-out and the mere presence and absence of meropenem specific peaks is not suitable as the only read-out.…”
Section: Assays Using Defined Marker Peaks To Deduce Susceptibility Omentioning
confidence: 99%