Evaluation of a monolithic epoxy silica support for penicillin G acylase immobilization

Integration of Biomolecules into analytical Systems by Means of Silica Sol-Gel Technology

“…Penicillin G acylase was immobilized on the activated monolith, and the column was used for enantioseparation of a series of 2-aryloxyalkanoic acids. 73 Trypin has also been attached to this monolithic support and used for the continuous online digestion of human serum albumin (HSA). 74 The most significant factor which affects the enzymatic activity is the flow rate.…”

Immobilization of Lipase on Epoxy Activated (1→3)-α-D-Glucan Isolated fromPenicillium chrysongenum

“…Common immobilization techniques include physical adsorption, 3) physical entrapment with a polymer support, and covalent bonding to a solid support. 4) However, several problems are associated with enzyme immobilization, such as steric interference, loss of protein activity, and changes in net surface charge of the support. Sometimes the amount of protein bound to a support does not always correlate with the activity recovered after immobilization.…”

Development of immobilized systems with penicillin acylase from Esherichia coli for production of semisyntetic penicillins

“…Penicilin-acilaza je takođe korisna za razdvajanje alkohola, -hidroksi--amino kiselina, -amino kiselina, i za zaštitu fenilacetil grupe u sintezi peptida. Osim toga, PAC može da vrši razdvajanje racemskih smeša hiralnih jedinjenja i ispolji umerenu do odličnu sposobnost stereohemijskog razlikovanja među odgovarajućim enantiomerima u hidrolitičkom raskidanju fenil-acetil grupe iz -aminoalkilfosfornih kiselina, -, -i -amino karboksilnih kiselina, šećera, amina, peptida i estara fenilsirćetne kiseline [8].…”

“…PAC katalizuje hidrolitičke reakcije pri blago alkalnim pH vrednostima (7,(5)(6)(7)(8)0) i reakcije sinteze pri nižim pH vrednostima (4,0-7,0). Prema tome, pri sintezi β-laktamskih antibiotika, koja se izvodi pri kiselim pH vrednostima, stabilnost enzima je od vitalnog značaja.…”

“…[129]. Grupa istraživača je ispitivala imobilizaciju PAC na ovako tretiran silicijum-dioksid i utvrdili su da se oko 180 mg enzima imobiliše po g mikročestica silicijum-dioksida (prečnika 5 m), izražavajući aktivnost od oko 1250 BPU/g.…”