Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping

Kérouanton, Annaëlle; Marault, Muriel; Petit, Laëtitia; Grout, Joël; Dao, Trinh Tam; Brisabois, Anne

doi:10.1016/j.mimet.2009.11.008

Cited by 129 publications

(104 citation statements)

References 18 publications

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“…These factors play a key role in the invasion and intracellular proliferation in animal tissues. The use of the PCR method enables identification a number of genes specific for L. monocytogenes species, such as hly, iap, prs, prfA, flaA, and actA (3,8,10,13,15,17,21).…”

Section: Discussionmentioning

confidence: 99%

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Budniak

Kędrak-Jabłońska

Szczawińska

et al. 2016

Journal of Veterinary Research

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Introduction: The aim of the study was to optimise and compare two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples including the liver, brain, and blood. Material and Methods: Three strains of L. monocytogenes and single strains of each of the species:welshimeri, and L. seeligeri were used. Additionally, five other species of bacterium were used to evaluate the specificity of the tests. Results: Specific amplification products were obtained for both multiplex PCR assays, which confirmed the tested strains as Listeria spp. and L. monocytogenes, respectively. Isolates of other species did not yield PCR products. Conclusion: Both multiplex PCR assays proved to be significantly sensitive and highly-specific methods for the detection of Listeria strains.

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Section: Discussionmentioning

confidence: 99%

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Budniak

Kędrak-Jabłońska

Szczawińska

et al. 2016

Journal of Veterinary Research

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“…Pulsed-field gel electrophoresis (PFGE) typing has rapidly become the standard subtyping method for investigating listeriosis outbreaks [8]. Nevertheless, for epidemiological monitoring of food and human isolates, serotyping is still required as a first level of discrimination [9]. Although 13 serovars are described for the species L. monocytogenes [10], at least 95% of the strains isolated from clinical and food samples belong to serovars 1/2a, 1/2b, 1/2c and 4b [2,11,12].…”

Section: Listeria Monocytogenes (L Monocytogenes) Is Amentioning

confidence: 99%

Listeria monocytogenes serotypes in human infections (Italy, 2000-2010)

Pontello

Guaita

Sala

et al. 2012

Annali Dell'Istituto Superiore Di Sanità

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Abstract.Background. In developed countries invasive listeriosis is an infection of great concern to public health to due its clinical severity and high fatality rate, despite its low incidence. In Europe, statistically significant increasing trends in listeriosis notification rates from 2005 to 2009 were noted in Austria, Denmark, Hungary, Italy, Spain and Sweden. Materials and methods. The standardized techniques based on phenotype to typing Listeria monocytogenes is the serotyping. In Europe, as elsewhere in the world, about 95% of L. monocytogenes strains isolated from clinical and food samples belongs to serovars 1/2a, 1/2b, 1/2c and 4b. Results. The target of this work is to draw attention to this important and atypical foodborne disease, reporting epidemiological data and serotypes distribution of 251 human L. monocytogenes isolates reported during 2000-2010 to Veterinary Public Health and Food Safety Department of Istituto Superiore di Sanità, focusing on epidemiological trend of invasive listeriosis in Lombardia, a North Italian Region. The serotypes most frequently identified are 1/2a, 4b, 1/2b (in total 92%), but the detection of uncommon serotypes is not missing (1/2c, 3a, 3b, 4d). Conclusions. In Italy the surveillance laboratory network, as well as the foodborne disease network (ENTER-NET), has revealed in the last 11 years an increase trend of listeriosis cases reported likewise with results of Notificable National Infectious Disease surveillance System. This is probably due to a real increase of listeriosis, even if there is a greater sensitivity of the network in some regions.Key words: Listeria monocytogenes, serotypes, surveillance. (2000)(2001)(2002)(2003)(2004)(2005)(2006)(2007)(2008)(2009)(2010). Introduzione. Nei Paesi industrializzati, nonostante la sua bassa incidenza la listeriosi invasiva è un'infezione alimentare di grande impatto per la salute pubblica, a causa della gravità delle sue manifestazioni cliniche e dell'alto tasso di letalità. In Europa, fra il 2005 e il 2009, sono stati notati incrementi, statisticamente significativi, delle notifiche di listeriosi in Austria, Danimarca, Ungheria, Italia, Spagna e Svezia. Materiali e metodi. Dal punto di vista fenotipico la tipizzazione sierologica rappresenta la tecnica più tradizionale per classificare gli stipiti di Listeria monocytogenes. In Europa come nel resto del mondo, il 95% circa dei ceppi di L. monocytogenes isolati da campioni clinici e alimentari appartengono ai sierotipi 1/2a, 1/2b, 1/2c e 4b. Risultati. L'obiettivo di questo studio è quello di attirare l'attenzione su questa grave e atipica malattia alimentare, riportando i dati epidemiologici e la distribuzione dei sierotipi, relativamente a 251 stipiti raccolti nel periodo 2000-2010, dal Dipartimento di Sanità Pubblica Veterinaria e Sicurezza Alimentare dell'Istituto Superiore di Sanità, focalizzando l'attenzione sui dati provenienti dalla Regione Lombardia. I sierotipi più frequentemente identificati sono 1/2a, 4b, 1/2b (per un complessivo 92%) ma non è mancato il risc...

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“…It is worth noticing that the 3 L. monocytogenes isolates from souvlaki samples were potentially identified as 4b serotype, which is the serotype most commonly causing human listeriosis (Kathariou, 2002;Gray et al, 2004;Liu, 2006). As for as the remaining 3 untypable isolates, they may belong to some clones of atypical strains (1/2a, 3a, 1/2c) which are not recognized in the expected PCR-types, using Doumith's assay (Kérouanton et al, 2010).…”

Section: Salmonellamentioning

confidence: 99%

Incidence and antimicrobial susceptibility of Salmonella, Listeria, and Campylobacter spp. in raw “souvlaki” marketed in Thessaloniki (Northern Greece)

Soultos

Iossifidou

Psomas

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. Souvlaki is a popular Greek meat product consisting of small chunks or cubes of pork threaded on a small wooden or metal skewer. In the present study, 105 samples of raw pork souvlaki obtained from retail shops in Thessaloniki (Northern Greece) were screened for the incidence of Salmonella, Listeria, and Campylobacter and their susceptibility to various antimicrobial agents; serotyping of the isolates was also performed. Of the samples tested, 1.9% were positive for Salmonella and yielded 3 serovars (S. SaintPaul, S. Fyris and S. Typhimurium); 31.4% proved positive for Listeria spp. with 6.7% yielding L. monocytogenes isolates belonged to molecular serogroups 2 (serotypes 1/2c and 3c) and 4 ( serotypes 4b, 4d, and 4e). Campylobacter spp. were not detected in any of the samples tested. The antimicrobial susceptibility to various antimicrobial agents of 11 Salmonella strains and 7 L. monocytogenes strains was also determined by disc diffusion method. Salmonella spp. were susceptible to a panel of 12 antibiotics but displayed intermediate resistance to tetracycline. L. monocytogenes isolates were resistant to nalidixic acid and ceftriaxone, partly resistant to clindamycin and cefotaxime, but sensitive to all antibiotics commonly used in veterinary and human listeriosis. Our findings indicate that souvlaki could be a potential vehicle of food borne infections due to strains of L. monocytogenes and Salmonella spp. in the case of inadequate thermal processing. In addition, good hygienic practices must be applied to avoid cross-contamination during preparation or handling of the product. Keywords

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Evaluation of a multiplex PCR assay as an alternative method for Listeria monocytogenes serotyping

Cited by 129 publications

References 18 publications

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Listeria monocytogenes serotypes in human infections (Italy, 2000-2010)

Incidence and antimicrobial susceptibility of Salmonella, Listeria, and Campylobacter spp. in raw “souvlaki” marketed in Thessaloniki (Northern Greece)

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