Evaluation of a Multiplex PCR assay for simultaneous identification ofSalmonellasp.,SalmonellaEnteritidis andSalmonellaTyphimurium from environmental swabs of poultry houses

Soumet, Christophe; Ermel, Gwennola; Rose, Nicolas; Rose, Valérie; Drouin, P.; Salvat, Gilles; Colin, P.

doi:10.1046/j.1365-2672.1999.00488.x

Cited by 121 publications

(82 citation statements)

References 7 publications

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“…The sefC gene encodes an outer membrane protein that contains the sefA subunit and the sefD adhesin. These results were similar to those obtained in another study in France [39].…”

Section: Discussionsupporting

confidence: 92%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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Introduction: Salmonella serovars are one of the primary foodborne pathogens. Poultry consumption is responsible for the majority of disease cases worldwide. The prevalence of virulence determinants among Salmonella serovars appears to be lacking in Egypt. Therefore, this study investigated the occurrence, antibiotic resistance patterns, and virulence gene profiling of Salmonella serovars in broilers. Methodology: Three hundred samples from broiler chickens were examined for the presence of Salmonella by standard microbiological techniques. All Salmonella isolates were tested for their sensitivity against ten antibiotics and subjected to virulence genotyping by polymerase chain reaction (PCR). Results: The overall isolation percentage of Salmonella was 17%. Seven different serovars were found, with the main one being Salmonella Typhimurium (52.94%). Salmonella isolates were sensitive to most of the tested antibiotics, but they exhibited absolute resistance against amoxicillin/clavulanic acid. Nine Salmonella strains (52.94%) were resistant to at least three antibiotics. Further PCR investigations into 17 Salmonella strains revealed different distribution patterns of eight virulence determinants among the isolates. The invA gene was the most prevalent one (100%), followed by hilA (88.24%), stn (58.82%), and fliC genes (52.94%), while each of sopB and pefA genes had a similar prevalence (41.18%), and sefC and spvC genes had the lowest prevalence (11.76 and 5.88%, respectively). PCR genotyping allowed grouping of Salmonella strains into ten genetic profiles. Conclusions: These results will help in understanding the spread of virulence genotypes and antibiotic resistance among Salmonella serovars in broilers.

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“…The sefC gene encodes an outer membrane protein that contains the sefA subunit and the sefD adhesin. These results were similar to those obtained in another study in France [39].…”

Section: Discussionsupporting

confidence: 92%

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Ammar

Mohamed

El-Hamid

et al. 2016

J Infect Dev Ctries

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“…and Salmonella spp. were identified by PCR as previously described [10][11][12]. DEC, in particular enteroagreggative (EAEC), enterotoxigenic (ETEC) and enteropathogenic E. coli (EPEC) were identified by multiplex PCR as previously described [13].…”

Section: Pathogen Detectionmentioning

confidence: 99%

Etiology, epidemiology and clinical characteristics of acute diarrhea in hospitalized children in rural Peru

Cornejo-Tapia

Orellana-Peralta

Weilg

et al. 2017

J Infect Dev Ctries

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Introduction: Diarrhea remains one of the main causes of morbidity and mortality in children under five years of age especially in low-income countries. In Peru, epidemiological reports about enteropathogens related to acute diarrhea are scarce in rural areas. The aim of this study was to describe the etiology, epidemiology, and clinical characteristics of the principal causes of acute infectious diarrhea in in a northern rural region of Peru. Methodology: A prospective study was conducted from January 2011 to December 2012 to describe the main pathogens causing acute diarrhea using PCR assay. Results: A total of 117 children diagnosed with acute diarrhea were included in the study. A single etiological agent was identified in 41.03% of samples, being rotavirus followed by norovirus and Shigella. Co-infections containing virus and bacteria were found in 22.22% of samples. Vomiting was most commonly found symptoms in 58.97% cases followed by fever (54.70%). Malnutrition was detected in 14.53% of the children. Conclusions: High prevalence of rotavirus, as well as adenovirus and norovirus, was observed in the present study. Shigella was the most common bacteria found in acute diarrhea in the area. The implementation of a better surveillance system is mandatory in order to identify the principal etiologies of gastroenteritis in the rural areas of Peru and to develop of better prevention strategies and reduce diarrhea-associated mortalities.

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“…51304, according to kit manufacturing. After extraction of genomic DNA from non-treated control cells, PCR was performed [23]. Preparation of PCR reaction mix was according to EmeraldAmp GT PCR mastermix (Takara) Code No.…”

Section: Isolation and Quantification Of Dna By Polymerase Chain Reacmentioning

confidence: 99%

Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens

El-Safty

Mahmoud

Zaki

et al. 2017

Asian J Pharm Clin Res

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Objective: Salmonella enteritidis ghosts (SEGs) is a non-living empty bacterial cell envelopes which were generated using a different concentration of sodium hydroxide (NaOH) 6.4 mg/mL and evaluated as a vaccine candidate in specific pathogen-free (SPF) chicken. SEGs have been produced by chemical-mediated lysis and evaluated the potential efficacy of chemically induced SEG vaccine and its ability to induce protective immune responses against virulent S. enteritidis challenge in SPF chickens.Methods: SPF chickens were divided into three groups: Group A (non-vaccinated control), Group B (vaccinated with prepared vaccine), and Group C (vaccinated with commercial vaccine). Results:Vaccination of SPF chicken with SEGs induced higher immune responses before and after virulent challenge. SPF chicken vaccinated with SEGs showed increasing in serum enzyme-linked immunosorbent assay (ELISA) antibodies. During the vaccination period, Groups B and C showed higher serum antibody titer compared to Group A. The minimal inhibitory concentration (MIC) of NaOH was capable of inducing non-living SEGs, and it has successfully generated non-living SEGs by MIC of NaOH. Conclusion:It is a one-step process which means easy manufacturing and low production cost compared to protein E-mediated lysis method. Chemically induced SEG vaccine is a highly effective method for inducing protective immunity. This study strongly suggests that SEGs will be a permissive vaccine, as the method of inhibition of S. enteritidis was safe and cheaper than other methods, and it gave a good protection.

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Evaluation of a Multiplex PCR assay for simultaneous identification ofSalmonellasp.,SalmonellaEnteritidis andSalmonellaTyphimurium from environmental swabs of poultry houses

Cited by 121 publications

References 7 publications

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Virulence genotypes of clinical SalmonellaSerovars from broilers in Egypt

Etiology, epidemiology and clinical characteristics of acute diarrhea in hospitalized children in rural Peru

Preparation and Evaluation of Chemically Inactivated Salmonella Enteritidis Vaccine in Chickens

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