Evaluation of a New Antigen for Diagnosis of Helicobacter pylori Infection in Stool of Adult and Children

Pourakbari, Babak; Mirsalehian, Akbar; Maleknejad, Parviz; Mamishi, Setareh; Azhdarkosh, Hossein; Daryani, Naser Ebrahimi; Najafi, Moslem; Kazemi, Bahram; Paknejad, Malieh; Mahmoudi, Shima; Bandehpour, Mojgan; Ghazi, Mona; Salavati, Ali

doi:10.1111/j.1523-5378.2010.00813.x

Cited by 26 publications

(17 citation statements)

References 32 publications

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“…It has been reported that detection of H. pylori antigens in stool is a suitable noninvasive method for clinical and epidemiologic studies (Koletzko, 2005; Pourakbari et al , 2011). …”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests

Pourakbari¹,

Ghazi²,

Mahmoudi³

et al. 2013

Braz. J. Microbiol.

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Although several invasive and noninvasive tests have been developed for the diagnosis of Helicobacter pylori infection, all of the tests have their limitations. We conducted a study to investigate and compare the suitability of rapid urease test (RUT), serology, histopathology and stool antigen tests with polymerase chain reaction (PCR) for detection of H. pylori, and correlate the diagnostic methods with PCR. Eighty nine patients (61 adults, 28 children) referred to the Firoozgar Hospital and Children Medical Center Hospital for diagnostic upper gastrointestinal endoscopy entered to the study and noninvasive tests such as immunoassay for serological antibodies against H. pylori and detection of its antigen in feces were measured. The biopsies were utilized for histological examination, RUT and PCR. The H. pylori statuses were evaluated by the positivity of ureC PCR in biopsy specimens and 53 subjects had H. pylori positive result. Histopathology showed high overall performance in adults and children with sensitivity and specificity 100% and 90%, respectively. Sensitivity, specificity, and accuracy for stool antigen test were 87.8%, 75% and 82%, respectively. Correlation of RUT, serology (IgG), histopathology and stool antigen tests with PCR were 0.82, 0.32, 0.91 and 0.63, respectively. In conclusion, the RUT and histopathology are as accurate as the PCR of biopsy and stool antigen test can consider as appropriate noninvasive test for detection of H. pylori infection.

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“…It has been reported that detection of H. pylori antigens in stool is a suitable noninvasive method for clinical and epidemiologic studies (Koletzko, 2005; Pourakbari et al , 2011). …”

Section: Discussionmentioning

confidence: 99%

Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests

Pourakbari¹,

Ghazi²,

Mahmoudi³

et al. 2013

Braz. J. Microbiol.

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“…HpSA is another non-invasive method with acceptable sensitivity and specificity for clinical and epidemiologic studies that its use has increased in recent years. 19 In this study we estimated the accuracy of HpSA for detecting H. pylori infection and observed that HpSA had sensitivity, specificity, PPV, NPV and accuracy of 96.67%, 93.33%, 93.55%, 96.55% and 95.00%, respectively.…”

mentioning

confidence: 96%

Role of Helicobacter Pylori stool antigen test in the diagnosis of Helicobacter Pylori infection

Pourlak¹,

Shokouhi²,

Vahedi³

2017

J Anal Res Clin Med

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Stool Antigen Test, AccuracyHelicobacter pylori (H. pylori) infection is common in developing countries. There are various invasive and non-invasive methods for diagnosing H. pylori infection. Stool antigen test is a new non-invasive method to diagnose H. pylori infection. In this study we evaluated the diagnostic accuracy of HpSA for H. pylori infection in patients with gastrointestinal (GI) complaints.Sixty patients including 28 men and 32 women with mean age of 49.2 ± 19.8 years undergoing diagnostic endoscopy with 30 positive and 30 negative rapid urease test (RUT) and histology results for H. pylori infection were evaluated. H. pylori stool antigen test (HpSA) was measured in these patients and sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and accuracy for HpSA were calculated.Among 31 positive HpSA results, 2 were negative in RUT and histology and among 29 negative HpSA results, 1 was positive in RUT and histology results. Sensitivity, specificity, PPV, NPV and accuracy for HpSA in diagnosing H. pylori infection were 96.67%, 93.33%, 93.55%, 96.55% and 95%, respectively.Although the sensitivity and specificity of the HpSA are evaluated in a relatively small sample size, these results showed that HpSA is a useful test in diagnosing H. pylori infection in patients with upper GI complaints.

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“…8 Epidemiologic studies showed that H. pylori affects over 50% of the world's population, infecting 40% to 50% of the population in industrialized countries and 70% to 90% in developing countries. 10 Invasive tests include endoscopy with gastric biopsy specimens, whereas noninvasive tests include serology, the urea breath test, the stool antigen test, and the polymerase chain reaction test (PCR). Several invasive and noninvasive diagnostic tests are used for the detection of H. pylori infection.…”

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confidence: 99%

“…12 The culture and identification of the organism is time-consuming but advantageous in that antibiotic susceptibility tests can be conducted. 10 Antigen detection in stool is an attractive noninvasive method that seems very suitable for clinical and epidemiologic studies as it can diagnose active infection in human fecal samples. 13 Nowadays, there is increasing interest in noninvasive methods to diagnose H. pylori infection.…”

mentioning

confidence: 99%

Evaluation of Noninvasive Versus Invasive Techniques for the Diagnosis of Helicobacter pylori Infection

El-Din

Hashem²,

Ragab³

et al. 2013

Applied Immunohistochemistry &Amp; Molecular Morphology

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Evaluation of a New Antigen for Diagnosis of Helicobacter pylori Infection in Stool of Adult and Children

Abstract: Using of AhpC antigen for diagnosis of H. pylori infection is a useful noninvasive method, accurate in adolescents and children, and can be used for the development of a stool antigen detection kit for H. pylori.

Cited by 26 publications

References 32 publications

Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests

Diagnosis of Helicobacter pylori infection by invasive and noninvasive tests

Role of Helicobacter Pylori stool antigen test in the diagnosis of Helicobacter Pylori infection

Evaluation of Noninvasive Versus Invasive Techniques for the Diagnosis of Helicobacter pylori Infection

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