Evaluation of a New Chromogenic Medium, MRSA
            <i>Select</i>
            , for Detection of Methicillin-Resistant
            <i>Staphylococcus aureus</i>

Louie, Lisa; Soares, Deirdre; Meaney, Helen; Vearncombe, Mary; Simor, Andrew E.

doi:10.1128/jcm.01763-06

Cited by 29 publications

(28 citation statements)

References 23 publications

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“…Van Hal et al (28,29), who used PCR detection of MRSA for comparison in two studies, found recovery rates of around 75% for the selective media. Groups that compared only one selective medium with nonselective media reported recovery rates above 90% (9,12,19,24,27). Hence, comparison of results gained from different studies can only be performed very cautiously.…”

Section: Discussionmentioning

confidence: 99%

“…The differences between the sensitivities of different chromogenic media may be caused by additives in the media that inhibit growth of the concomitant microorganisms but that also inhibit the growth of MRSA (25). However, groups that compared different media usually did not compare the recovery rates for different specimens on selective media to those on nonselective media (19,22,26,29).…”

Section: Discussionmentioning

confidence: 99%

“…The recovery of MRSA from different kinds of samples has been compared in a few other studies (19,22,26,29). For some types of specimens, the recovery rate was as low as 50% (29), but this does not seem to be equally distributed for different brands of selective media.…”

Section: Discussionmentioning

confidence: 99%

“…Since there is a lack of a gold standard for MRSA screening, most authors compared the recovery rate of a specific medium with the combined result of all media or methods that have been used (5,6,9,10,12,13,15,19,20,(21)(22)(23)(24)(26)(27)(28)(29)(30). This prevents the misclassification of true-positive results, i.e., detection of MRSA classified as false positive if the comparison method failed to detect MRSA.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of a New Selective Medium, BD BBL CHROMagar MRSA II, for Detection of Methicillin-Resistant Staphylococcus aureus in Different Specimens

et al. 2010

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The sensitivity of screening for methicillin-resistant Staphylococcus aureus (MRSA) can be improved by adding other specimen sites to nares. We describe an evaluation of a new selective medium, BBL CHROMagar MRSA II (CMRSAII), for its ability to detect MRSA from different specimen types. CMRSAII is a chromogenic medium which incorporates cefoxitin for the detection of MRSA. A study was performed at four clinical laboratories with the following specimens: 1,446 respiratory, 694 stool, 1,275 skin, and 948 wound specimens and 688 blood culture bottles containing Gram-positive cocci. The recovery of MRSA on traditional culture media was compared to results with CMRSAII. S. aureus was tested by cefoxitin disk diffusion. CMRSAII was interpreted as positive for MRSA at 24 h (range, 18 to 28 h) based solely on the visualization of mauve-colored colonies and at 48 h (range, 36 to 52 h) based on detection of mauve colonies with subsequent confirmation as S. aureus (by coagulase or latex agglutination testing). MRSA was recovered more frequently on CMRSAII (89.8% at 24 h and 95.6% at 48 h) than on traditional culture plates (83.1% at 24 h and 79.8% at 48 h) for all specimen types combined (P < 0.001). The percent sensitivities of CMRSAII at 24-and 48-h reads, respectively, were 85.5 and 92.4% for respiratory specimens, 87.9% and 98.3% for stool specimens, 88.4% and 96.1% for skin specimens, 92.1% and 94.6% for wound specimens, and 100% and 100% for positive blood cultures. The specificity was 99.8% for respiratory specimens and 100% for all others. In conclusion, CMRSAII is a reliable screening medium for multiple specimen types.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Evaluation of a New Selective Medium, BD BBL CHROMagar MRSA II, for Detection of Methicillin-Resistant Staphylococcus aureus in Different Specimens

et al. 2010

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“…, [9], [11,12] [7], [12,13] [9], [12] [6], [8][9][10][11][12] [6], [8,9], [11,12] [12] [7], [9][10][11], [13] [9], [11], [13] [9] [6,7], [9], [12,13] …”

mentioning

confidence: 99%

Active Surveillance for Multidrug-resistant Organisms

et al. 2013

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Infections and outbreaks of antimicrobial-resistant bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococcus (VRE), have been increasing. Detection methods for antimicrobial-resistant bacteria have been changed from traditional culture methods to chromogenic media culture and molecular methods. Straintyping methods using various molecular technologies are essential tools for epidemiologic surveillance. Furthermore, outbreak detection, using syndromic surveillance as well as passive and active surveillance, has been applied. However, it is difficult to establish effective and robust guidelines and systems for using these various methods to control antimicrobial-resistant bacteria. Therefore, clinical microbiologists and policy makers must possess expertise in the control of antimicrobial resistant bacteria, discuss the issue sufficiently, and, finally, create a system to accomplish this control. (Ann Clin Microbiol 2013;16:53-60)

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Optimal Use of MRSASelect and PCR to Maximize Sensitivity and Specificity of MRSA Detection

et al. 2012

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Suspected colonies of methicillin-resistant Staphylococcus aureus (MRSA) on chromogenic, MRSASelect (BioRad) medium were confirmed using routine microbiological methods, and a multiplex real-time PCR (n = 108). Although the specificity of MRSASelect assessed at 24 h of incubation was much higher than that of 48 h (91.4 vs. 60 %), extending the incubation time to 48 h, along with PCR confirmation, increased the total number of true positive samples by 27.8 %. These results provide a cost effective method for sensitive and specific detection of MRSA.

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Evaluation of a New Chromogenic Medium, MRSA Select , for Detection of Methicillin-Resistant Staphylococcus aureus

Cited by 29 publications

References 23 publications

Evaluation of a New Selective Medium, BD BBL CHROMagar MRSA II, for Detection of Methicillin-Resistant Staphylococcus aureus in Different Specimens

Evaluation of a New Selective Medium, BD BBL CHROMagar MRSA II, for Detection of Methicillin-Resistant Staphylococcus aureus in Different Specimens

Active Surveillance for Multidrug-resistant Organisms

Optimal Use of MRSASelect and PCR to Maximize Sensitivity and Specificity of MRSA Detection

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