Evaluation of A New Enzyme Immunoassay for Detecting Helicobacter Pylori in Feces: A Prospective Pilot Study

Trevisani, Lucio; Sartori, Sergio; Galvani, Fabrizio; Rossi, Maria Rita; Ruina, Marco; Chiamenti, C; Caselli, Michele

doi:10.1111/j.1572-0241.1999.01213.x

Cited by 88 publications

(63 citation statements)

References 8 publications

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“…H. pylori has been cultured from the feces of experimentally colonized individuals (13) and from children in The Gambia (20), but such culturing does not appear to be easy for the majority of patients. Several studies have detected H. pylori antigens in fecal material (15,22,23), but this technique cannot provide information beyond the presence or absence of antigens.…”

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confidence: 99%

Evaluation of Protocol Using Gene Capture and PCR for Detection of Helicobacter pylori DNA in Feces

et al. 2003

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The route of transmission of Helicobacter pylori, which is usually acquired in childhood and is one of the most common bacterial infections in humans, remains undetermined. Mapping the distribution of H. pylori genotypes within families could help to determine the routes of transmission and risk factors. Here we describe a noninvasive method for obtaining H. pylori DNA isolates from the feces of children. Children presenting with gastrointestinal symptoms at the Royal Hospital for Sick Children were tested for gastric H. pylori colonization by using the 13 C-urea breath test (UBT) and were asked to provide fecal samples, which were tested for H. pylori by using the HpSA fecal antigen test. DNA was purified from fecal samples by using a novel method of gene capture with subsequent H. pylori PCR analysis. Fifteen UBT-positive and 15 UBT-negative children participated in the study. The positive and negative predictive values for the assay were 80 and 100%, respectively. Fecal DNA purification followed by H. pylori PCR analysis is an effective tool for harvesting H. pylori DNA isolates from the feces of children. This technique may be developed to allow the diagnosis and noninvasive genotyping of H. pylori in children and their families.

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Evaluation of Protocol Using Gene Capture and PCR for Detection of Helicobacter pylori DNA in Feces

et al. 2003

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“…Although some investigators have demonstrated that UBT and HpSA are absolutely equivalent in terms of sensitivity and specificity in the evaluation of eradication therapy, 15,23,24 others have documented a lower than expected reliability of HpSA in this setting. [25][26][27][28][29] It has been rightly emphasized that many studies on the performance of HpSA in post-treatment assessment have used UBT as the gold standard, and this has certainly diminished the meaning of the results. 30,31 However, the few studies using biopsy-based methods as the gold standard have provided conflicting results.…”

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confidence: 99%

Stool antigen assay (HpSA) is less reliable than urea breath test for post‐treatment diagnosis of Helicobacter pylori infection

Bilardi¹,

Biagini²,

Dulbecco³

et al. 2002

Aliment Pharmacol Ther

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“…In previous studies, the HpSA test was investigated for the diagnosis of H. pylori infection and the sensitivity and specificity were reported to be over 80% in symptomatic untreated patients (Makristathis et al 1998;Puspok et al 1999;Trevisani et al 1999;Vaira et al 1999). In our study, the sensitivity of the HpSA test was found and specificity.…”

Section: Discussionmentioning

confidence: 99%

Quantitative Correlation of Helicobacter pylori Stool Antigen (HpSA) Test with the Severity of H. pylori-Related Gastritis

Adiloğlu

Işler

Goren

et al. 2007

Tohoku J. Exp. Med.

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The Helicobacter pylori (H. pylori) load in both stomach and stool and the resulting severity of gastritis are important criteria in validating the status of H. pylori infection. We aimed to assess the reliability of the H. pylori stool antigen (HpSA) test for the primary diagnosis of H. pylori infection by calculating the best cut-off value to obtain the highest sensitivity and specificity in dyspeptic patients. We also investigated the correlation of HpSA test with the severity of gastritis and H. pylori load. The H. pylori statuses of 95 patients were evaluated by the positivity of both rapid urease test and microscopic detection of H. pylori in biopsy specimens, 88 subjects of whom were H. pylori positive. The sensitivity and specificity of the HpSA test were 51.1% (45/88) and 100% (7/7), respectively, according to the manufacturer's recommended cut-off value of 0.16. However, with the best cut-off value of 0.048, calculated by receiver operator characteristics analysis, the sensitivity of the test increased to 92.0% (81/88) with the same specificity. High values of the HpSA test were correlated with high scores of corpus H. pylori load and the severity of antrum and corpus inflammation ( p < 0.05). With the best cut-off value of the HpSA test, the primary diagnosis of H. pylori infection can be made with higher sensitivity and specificity. The HpSA test is a helpful tool that evaluates the severity of H. pylori infection and the degree of gastric inflammatory activity and gastric H. pylori load.

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Evaluation of A New Enzyme Immunoassay for Detecting Helicobacter Pylori in Feces: A Prospective Pilot Study

Cited by 88 publications

References 8 publications

Evaluation of Protocol Using Gene Capture and PCR for Detection of Helicobacter pylori DNA in Feces

Evaluation of Protocol Using Gene Capture and PCR for Detection of Helicobacter pylori DNA in Feces

Stool antigen assay (HpSA) is less reliable than urea breath test for post‐treatment diagnosis of Helicobacter pylori infection

Quantitative Correlation of Helicobacter pylori Stool Antigen (HpSA) Test with the Severity of H. pylori-Related Gastritis

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