Evaluation of a New Hæmagglutination Technique for the Demonstration of Hepatitis-B Antigen

Reesink, H. W.; Duimel, W.J.; Brummelhuis, H. G. J.

doi:10.1016/s0140-6736(73)93322-9

Cited by 34 publications

(8 citation statements)

References 4 publications

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“…This is in contrast to other sensitive tests such as those based on haemagglutination reactions [21]. Since the sensitivity of the assay was found to be time dependent, it was necessary to incubate for 18-20 h. Although longer incubation times did increase the amount of bound tracer, the sensitivity had not increased significantly.…”

Section: Discussionmentioning

confidence: 92%

A New Solid Phase Radioimmunoassay (CLB-RIA) for the Detection of Hepatitis-B Antigen and Antibody

Duimel¹,

Brummelhuis²

1975

Vox Sanguinis

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A new competitive solid phase radioimmunoassay (CLB-RIA) has been developed for the detection of HBAg and HBAb in human serum and plasma. In the assay, sheep antibodies to HBAg, covalently linked to an insoluble carrier, highly purified 125 I labelled HBAg and the serum or plasma sample are incubated for 20 h at room temperature. After incubation, the bound and the free fraction of the tracer are separated by centrifugation. Both the presence of HBAg and HBAb, result in a decrease of the amount of bound tracer, when compared with a negative control serum. Differentiation between HBAg and HBAb requires the use of another type of radioimmunoassay. For this purpose a sandwich solid phase radioimmunoassay, for the detection of HBAb only, has been developed (CLB-AURIA). In this, assay-purified HBAg is covalently linked to an insoluble carrier. Using a mixture of both immunosorbents (insolubilized HBAg and HBAb), it is possible to detect and to distinguish HBAg and HBAb in one single solid phase radioimmunoassay (CLB-MIRIA). The influence of three parameters on the CLB-RIA, the incubation time, the amount of tracer and the effect of Tween-20 has been studied. The sensitivity of the described solid phase CLB-RIA for the detection of HBAg is comparable to that of other radioimmunoassays reported in literature; its specificity is very high.

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Section: Discussionmentioning

confidence: 92%

A New Solid Phase Radioimmunoassay (CLB-RIA) for the Detection of Hepatitis-B Antigen and Antibody

Duimel¹,

Brummelhuis²

1975

Vox Sanguinis

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“…All sera were heat-inactivated [14] and the test was carried-out according to the manufacturer's instructions. Positively reacting sera were re-tested after absorption with erythrocytes coated with sheep IgG without anti-HBsAg activity ("Hepanosticon Absorbent").…”

Section: Methodsmentioning

confidence: 99%

Immunodeficiency in Down's syndrome: Relationship between presence of human thyroglobulin antibodies and HBsAg carrier status

et al. 1977

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The relationship between the presence of hepatitis B surface antigen (HBsAg) and antibodies to human thyroglobulin (HTgAb) has been studied in 110 subjects with Down's syndrome (DS) from 4 months to 50 years of age and in 122 controls carefully matched for sex, age and socio-environmental conditions. The overall percentage of HBsAg carriers was 22.7 in DS and 6.6 in controls and that of HTgAb-positive subjects was 41.8 in DS and 19.7 in controls. In DS the frequency of HTgAb-positive subjects was very high, even in the youngest age groups in which the percentage of HBsAg carriers was relatively low; the latter thereafter showed a marked increase with age. A positive association between the presence of HBsAg and HTgAb was found only in the oldest age group of DS subjects. It is thus concluded that in DS the high frequency of HTgAb cannot be attributed to chronic hepatitis B virus infection. On the contrary, the presence of HTgAb might well represent an early "marker" of immunodeficiency and increased susceptibility to infection with hepatitis B virus.

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“…They all recovered from acute hepatitis and became negative for HBsAg. Thus 14 donations which had been available for normal transfusion on the strength of a negative reaction on C.I.E.P. were found to be HBsAg positive by R.I.A.…”

Section: Four-year Reviewmentioning

confidence: 99%

Which techniques should be used to screen blood donations for hepatitis B surface antigen?

Wallace¹,

Barr²,

Milne³

1975

BMJ

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Evaluation of a New Hæmagglutination Technique for the Demonstration of Hepatitis-B Antigen

Cited by 34 publications

References 4 publications

A New Solid Phase Radioimmunoassay (CLB-RIA) for the Detection of Hepatitis-B Antigen and Antibody

A New Solid Phase Radioimmunoassay (CLB-RIA) for the Detection of Hepatitis-B Antigen and Antibody

Immunodeficiency in Down's syndrome: Relationship between presence of human thyroglobulin antibodies and HBsAg carrier status

Which techniques should be used to screen blood donations for hepatitis B surface antigen?

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