Evaluation of a new rapid immunoassay for the detection of <i>Helicobacter pylori</i> in faeces: a prospective pilot study

Trevisani, Lucio; Sartori, Sergio; Rossi, Maria Rita; Ruina, Marco; Matarese, V.; Gullini, S.; Abbasciano, Vincenzo

doi:10.1111/j.1365-2036.2005.02355.x

Cited by 32 publications

(31 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

mentioning

confidence: 99%

“…In the last years interest has been focused on noninvasive methods, especially the detection of the pathogen in feces. The H. pylori stool antigen test provides a simple alternative to the urea breath test and is appropriate for diagnosis and follow-up of infection (3,9,11,12,14,(16)(17)(18). While the value of enzyme immunoassays (EIAs) using either polyclonal or monoclonal antibodies is well documented, the sporadic existing data for the first developed and commercially available rapid test (ImmunoCard STAT!…”

mentioning

confidence: 99%

“…While the value of enzyme immunoassays (EIAs) using either polyclonal or monoclonal antibodies is well documented, the sporadic existing data for the first developed and commercially available rapid test (ImmunoCard STAT! HpSA) showed enormous differences among the studies (1,7,8,14,17), and its reliability is reported to be somewhat lower than that of monoclonal fecal antigen EIA (2, 9).Rapid Hp StAR (DakoCytomation Ltd., United Kingdom) is a newly developed qualitative immunochromatographic membrane-based assay using monoclonal antibodies and amplification technology for the direct detection of H. pylori antigens in human feces. The test has two capture lines, one coated with an H. pylori-specific amplified capture reagent (test line) and one with a control capture reagent (control line).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

2008

View full text Add to dashboard Cite

The evaluation of a new rapid stool antigen test showed different levels of sensitivity for final readings of test results at 20 min (59.1%) and 30 min (76.9%). Significant differences in performance were observed between the two sexes and the various age categories, with higher efficiency in male patients and young adults. Generally, this test is efficient and can be used to detect H. pylori infection in adults. However, further studies are required to confirm its accuracy.Helicobacter pylori is well recognized as a major cause of gastrointestinal diseases (10,11). Patients with successful eradication therapy show evidence for this cause-and-effect relationship; gastritis and ulcers often are cured, and the risk of recurrence is greatly reduced (4). Therefore, reliable detection of H. pylori infection is of major importance. In the last years interest has been focused on noninvasive methods, especially the detection of the pathogen in feces. The H. pylori stool antigen test provides a simple alternative to the urea breath test and is appropriate for diagnosis and follow-up of infection (3,9,11,12,14,(16)(17)(18). While the value of enzyme immunoassays (EIAs) using either polyclonal or monoclonal antibodies is well documented, the sporadic existing data for the first developed and commercially available rapid test (ImmunoCard STAT! HpSA) showed enormous differences among the studies (1,7,8,14,17), and its reliability is reported to be somewhat lower than that of monoclonal fecal antigen EIA (2, 9).Rapid Hp StAR (DakoCytomation Ltd., United Kingdom) is a newly developed qualitative immunochromatographic membrane-based assay using monoclonal antibodies and amplification technology for the direct detection of H. pylori antigens in human feces. The test has two capture lines, one coated with an H. pylori-specific amplified capture reagent (test line) and one with a control capture reagent (control line). A recent study reported good performance with posttreatment patients (14). No data are available on its reliability as a screening test for primary diagnosis. Moreover, the results of stool antigen tests may vary according to geographical locations (3, 9, 12). The objective of this trial was to evaluate the performance of this novel test with a group of dyspeptic adults in our region, in comparison to a well-defined H. pylori status established by invasive diagnostic methods. To determine sources of heterogeneity that may have an influence on applicability, the test was evaluated in relation to clinical manifestations and the ages and genders of the patients.(The results of this study were presented in part at the 17th European Congress of Clinical Microbiology and Infectious Diseases, Munich, Germany, 2007.) A total of 72 consecutive patients (37 females and 35 males) (mean age, 58.4 Ϯ 12 years; range, 24 to 88 years) who were referred to the Department of Surgery at the University Hospital of Kiel and to a gastroenterological private practice were enrolled in the study between 2002 and 2003. All patients who pre...

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

2008

View full text Add to dashboard Cite

show abstract

“…Monoklonal antikor içeren ELISA ve monoklonal immunokromatografik yöntemi karşılaştıran bir çalışmada (23) immunokromatografik yönte-min duyarlılığının düşük (% 63.8), ELISA yönte-minin duyarlılığının ise oldukça yüksek (% 98) olduğu bildirilirken diğer çalışmalarda immunokromatografik yöntemle ELISA yönteminin duyarlılıklarının benzer olduğu ve immunokromatografik testlerin tanıda kullanılabileceği bildirilmiştir (21,24) . Daha önce yaptığımız bir çalış-mada monoklonal ELISA yönteminin duyarlılık ve özgüllüğü sırasıyla % 90 ve % 96 olarak bildirilmiştir (11) .…”

Section: Discussionunclassified

Immunochromatographic Tests Detecting Helicobacter pylori Antigen in Stool: Comparison of the Results of Eight Different Commercial Kits

İlktaç¹,

Şahin²,

Nazik³

et al. 2012

Ankem Derg

View full text Add to dashboard Cite

ÖZET GİRİŞDünyada en sık rastlanan infeksiyonlardan biri olan Helicobacter pylori infeksiyonlarının gastrit, peptik ülser, mide kanseri ve mukoza ile ilişkili lenfoid doku lenfoması gibi çeşitli gastroduodenal hastalıkların gelişmesinde rol oynadı-ğının kesinleşmesi ile birlikte bu bakterinin doğru ve hızlı tanısı oldukça önem kazanmış-tır (18) . H. pylori infeksiyonlarının tanısında histolojik inceleme, hızlı üreaz testi (HÜT), kültür ve PCR gibi endoskopi işlemi ile alınan mide biyopsi örneğinin incelenmesini gerektiren invaziv yöntemler ve seroloji, üre nefes testi ve dışkıda antijen saptayan ELISA testleri gibi invaziv olmayan yöntemler kullanılmaktadır (2,15,18) . İnvaziv yöntemlerden PCR yönteminin duyarlılığı % 95, histolojik incelemenin duyarlılığı % 90-95, HÜT'ün duyarlılığı % 80-95 ve kültür yöntemi-nin duyarlılığı ise % 75 oranında bildirilmekte-

show abstract

“…It is highly suitable for the detection of H. pylori in practice, because it is a rapid one-step single test. 17,18 There have been several validation studies on the rapid HpSA test in children. 12,19 However, only a few published studies have compared the diagnostic accuracy of the rapid HpSA test with that of the HpSA ELISA in children.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Enzyme-Immunoassay and Rapid Immunochromatography Test for Detecting Helicobacter pylori Stool Antigen

Prasetyo¹

2014

mkb

View full text Add to dashboard Cite

The rapid immunochromatography for Helicobacter pylori stool antigen (HpSA) test is a recently developed method for detection of Heicobacter pylori (H. pylori) infection. The objective of this study was to compare rapid HpSA test with a conventional enzyme-immunoassay (EIA) and to determine the prevalence of H. pylori infection in symptomatic (recurrent abdominal pain/RAP) Indonesian children. One hundred and two high school children aged 12-18 years old in Bandung with RAP from July to November 2008 were enrolled, excluding children who had additional symptoms, such as diarrhea or fever. First study was performed in 102 samples in laboratory of Leiden University Medical Center (LUMC), Helicobacter pylori infection was detected by a conventional EIA and rapid test. A second study was performed on a stored collection of 32 feces samples previously tested positive for H. pylori by EIA in laboratory of LUMC; these samples were retested by both assays. The results showed the sensitivity, specificity, positive predictive value, and negative predictive value of the rapid test were 100%, 98.2%, accuracy 98.5%, 92.6% and 100%, respectively, from 134 tested samples. The overall prevalence of H. pylori infection among Indonesian children was 3% by EIA at first testing. In conclusion, the prevalence of H. pylori infection among symptomatic Indonesian children is very low and the new developed Pylori-Strip test shows good performance, very fast, and easy to use compared to EIA. AbstrakPemeriksaan Helicobacter pylori stool antigen (HpSA) menggunakan rapid immunochromatography (rapid HpSA) merupakan metode yang saat ini sedang berkembang untuk mendeteksi infeksi H. pylori. Tujuan penelitian ini untuk membandingkan metode rapid HpSA dengan metode konvensional yaitu enzyme-immunoassay (EIA) dan menentukan prevalensi infeksi H. pylori pada anak dengan gejala sakit perut berulang (SPB) di Indonesia. Sebanyak 102 anak sekolah menengah usia 12-18 tahun di Bandung dengan keluhan SPB ikut dalam penelitian ini, dilaksanakan dari Juli-November 2008. Anak dengan gejala tambahan, seperti diare dan demam, dikeluarkan dari penelitian. Penelitian pertama dilakukan pada sampel feses 102 anak di laboratorium Leiden University Medical Center (LUMC), Helicobacter pylori terdeteksi baik dengan pemeriksaan EIA konvensional maupun rapid test. Penelitian kedua dilakukan pada 32 buah sampel feses simpanan di laboratorium LUMC yang sebelumnya menunjukkan hasil positif dengan pemeriksaan EIA, sampel ini juga diperiksa menggunakan kedua metode. Hasil penelitian menunjukkan sensitifitas, spesifisitas, akurasi, positive predictive value, dan negative predictive value, dengan hasil 100%, 98,2%, 98,5%, 92,6%, dan 100% dari 134 sampel yang diuji. Prevalensi infeksi H. pylori pada anak Indonesia dengan keluhan SPB sekitar 3% pada pemeriksaan pertama. Simpulan, prevalensi infeksi H. pylori pada anak SPB di Indonesia sangat sedikit dan pemeriksaan Pylori-strip (rapid test) merupakan pemeriksaan yang sangat baik dan cepat serta mudah digunakan dibandin...

show abstract

Evaluation of a new rapid immunoassay for the detection of Helicobacter pylori in faeces: a prospective pilot study

Cited by 32 publications

References 15 publications

Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

Evaluation of a Rapid New Stool Antigen Test for Diagnosis of Helicobacter pylori Infection in Adult Patients

Immunochromatographic Tests Detecting Helicobacter pylori Antigen in Stool: Comparison of the Results of Eight Different Commercial Kits

Comparison of Enzyme-Immunoassay and Rapid Immunochromatography Test for Detecting Helicobacter pylori Stool Antigen

Contact Info

Product

Resources

About