Evaluation of a new vaccine against Staphylococcus aureus mastitis in dairy herds of southern Chile. I. Challenge trial

Mella, A.; Ulloa, Fernando; Valdés, Iván; Olivares, Nicolás; Ceballos, Alejandro; Kruze, J.

doi:10.4067/s0719-81322017000300149

Cited by 9 publications

(7 citation statements)

References 26 publications

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“…For MRSA and E. coli strains, bacterial concentrations in each sample were determined using serial 10-fold dilutions plated on blood agar. From each plate, a colony typical of S. aureus was identified using Staphytec Plus (Oxoid Diagnostic Reagents, UK) [ 30 ], or a colony typical of E. coli was identified using a panel of biochemical tests, which included Triple sugar iron agar, Lysine iron agar, Motility indole ornithine medium, Simmon’s citrate agar and Urea agar (all from Oxoid Diagnostic Reagents, UK).…”

Section: Methodsmentioning

confidence: 99%

In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions

et al. 2018

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BackgroundMycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a contagious infectious disease that affects domestic and wild ruminants causing chronic inflammation of the intestine. MAP has proven to be very resistant to both physical and chemical processes, making it difficult to control this pathogen. Based on the recognized antimicrobial properties of copper, the objective of this study was to evaluate the effectiveness of copper ions to reduce MAP numbers and/or MAP viability in a fluid matrix. Besides, methicillin-resistant Staphylococcus aureus (MRSA), and Escherichia coli were used as controls of the effectiveness of copper ions. MAP-spiked PBS was subjected to copper ions treatment at 24 V for 5 min and the PBS suspensions were sampled before and after treatment. MAP viability and quantification were determined using three complementary techniques: a phage amplification assay, MGIT culture and qPCR.ResultsModerate numbers (103 CFU ml−1) of the two control bacteria were completely eliminated by treatment with copper ions. For MAP, copper ions treatment reduced both the viability and numbers of this pathogen. Phage assay information quickly showed that copper ions (24 V for 5 min) resulted in a significant reduction in viable MAP. MGIT culture results over time showed statistically significant differences in time-to-detection (TTD) values between PRE and POST treatment. MAP genome equivalent estimates for PBS suspensions indicated that MAP numbers were lower in samples POST-treatment with copper ions than PRE-treatment.ConclusionsThe use of copper ions resulted in a significant reduction of MAP in a liquid matrix, although some MAP survival on some occasions was observed.

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Section: Methodsmentioning

confidence: 99%

In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions

et al. 2018

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“…Furthermore, Ghobrial et al [36] investigated that Lysigin was successfully effective in eliminating 20% of S. aureus mastitis in an Egyptian dairy farm. On the other hand, previous results indicate that the vaccine does not entirely protect the udder against S. aureus mastitis [21].…”

Section: Discussionmentioning

confidence: 86%

“…Staphylococcus aureus is a major pathogen responsible for both clinical and subclinical mastitis in dairy cows [29]. The inability of the current antibiotics to manage the contagious S. aureus mastitis, as well as the growing worry about the continual development of antimicrobial resistance in milk highlight the need for antibiotic alternatives [21]. Vaccination against S. aureus mastitis has been investigated and advocated as a substantial helpful strategy for the management of staphylococcal infections in dairy cows [16].…”

Section: Discussionmentioning

confidence: 99%

“…Regardless of the vaccine type, vaccination alone is inadequate for preventing mastitis, particularly in dairy herds with high mastitis rates [20]. Therefore, vaccination as a good control strategy must be complemented with other traditional control programs focusing on hygiene and management to reduce the frequency and duration of mastitis cases [17,21]. The purpose of this study was to investigate the effect of vaccination with a commercially available S. aureus bacterin (Lysigin, Boehringer Ingelheim Vetmedica, Inc.) to reduce the rate of mastitis infection in a dairy farm in Egypt.…”

Section: Introductionmentioning

confidence: 99%

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Efficacy of Lysigin Vaccine in the Prevention of Mastitis in Dairy Cattle

Badr,

Bakry,

Al-Amry

et al. 2023

Veterinary Medical Journal (Giza)

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Mastitis is one of the biggest expenses for the dairy industry which has a substantial impact on dairy cow health. Preventing new infections in cows is the best strategy to manage mastitis. This study aimed to investigate the effect of a commercially available S. aureus bacterin (Lysigin) on minimizing the rate of mastitis in a dairy herd. A total number of 600 Holstein-Friesian dairy cows were involved in the study and received two doses of the vaccine. The enrolled animals were monitored for clinical signs, bacterial culture, somatic cell count (SCC), and costs utilized for treatment during the study. The rate of clinical and subclinical mastitis was reduced from 13% and 29% to 6.7% and 18.3%, respectively after one year following vaccination. Composite milk samples from infected cows were examined for bacteriological isolation of S. aureus and E. coli. S. aureus and E. coli mastitis were determined in percentages of 25% and 30.9%, respectively. Following immunization, S. aureus and E. coli mastitis were decreased to 10% and 23.3%, respectively. The decrease in the rate of E. coli may be related to farm hygiene and environmental management which has a detrimental effect in the control of coliform mastitis. The SCC on milk samples from animals with chronic infection significantly decreased after vaccination. Furthermore, a reduction in bulk milk tank SSC (BTSSC) was detected in the herd during the study. The application of vaccination has a greater impact on reducing the costs utilized for the treatment of mastitis in the herd. These findings indicate that the Lysigin vaccine has a protective effect against S. aureus mastitis and can be utilized as an additional approach for the management of mastitis.

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“…M. bovis causes more severe cases of intramammary infections due to Mycoplasma, and has been associated with respiratory infections, otitis, septic arthritis, and reproductive infections in cattle Ayling 2003, Maunsell et al 2011). Intramammary infection due to Mycoplasma bovis is feared by dairy farmers and veterinarians because it is highly contagious, hard to control and there are still no vaccines available, as for other udder pathogens (Mella et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Mycoplasma isolation in milk samples from dairy herds in Chile

Ulloa

Soto²,

Kruze

et al. 2021

Austral j. vet. sci.

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Mycoplasma bovine mastitis is a highly contagious disease, usually associated with clinical cases refractory to antibiotic treatment. The aim of this study was the isolation of Mycoplasma species in cattle milk samples from dairy herds in Chile. Bulk tank milk samples selected by convenience from 91 Holstein Friesian dairy herds located in Los Rios (66) and Los Lagos (25), the two most important dairy Regions in the country, were collected. Additionally, 100 individual milk samples from cows with a high incidence of clinical mastitis, refractory to antibiotic therapy, and negative bacteriological results for traditional mastitis pathogens, all from the Biobío Region and received in our diagnostic laboratory, were included. All samples were cultured for 10 days on PPLO medium. The differentiation of suspect colonies between genus Mycoplasma and Acholeplasma was performed by the digitonin test and a specific PCR. The species identification was performed by a M. bovis specific PCR and 16S rRNA sequencing. Mycoplasma was isolated from 3 (3.3%) bulk tank milk samples and 2 (2%) individual cow milk samples. All colonies were identified as Mycoplasma by the digitonin test and by a specific PCR. At species level, one strain isolated from a bulk tank milk sample was identified as M. bovis. The remaining two strains isolated from bulk tank milk samples were identified as M. bovigenitalium, while the two strains isolated from milk of individual cows were identified as M. alkalescens. These results show that not only M. bovis is present in Chilean dairy herds, but also other pathogenic species not previously described in Chile such as M. bovigenitalium and M. alkalescens, which pose a potential risk for dairy herds in southern Chile.

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Evaluation of a new vaccine against Staphylococcus aureus mastitis in dairy herds of southern Chile. I. Challenge trial

Cited by 9 publications

References 26 publications

In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions

In vitro inactivation of Mycobacterium avium subsp. paratuberculosis (MAP) by use of copper ions

Efficacy of Lysigin Vaccine in the Prevention of Mastitis in Dairy Cattle

Mycoplasma isolation in milk samples from dairy herds in Chile

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