Evaluation of a novel 2,3-diacyl-trehalose-2′-sulphate (SL-IV) antigen for case finding and diagnosis of leprosy and tuberculosis

Use of the 2,3-diacyl-trehalose and the purified protein derivative in the serodiagnosis of tuberculosis in AIDS

“…Although immunocompetent patients produce more antibodies to SL-IV than to PPD, the differences between the two antigens were not significant (p>0.05). Our data confirmed the results obtained by Cruaud et al (1990) on the evaluation of SL-IV antigen for case finding of TB in non-immunocompromised patients. The high specificity and high sensitivity achieved in the ELISA test based on the SL-IV in these groups were similar to those results observed by others, using other specific antigens (Daniel 1989, Wilkins & Ivanyi 1990.…”

“…The ELISA for SL-IV was performed as previously described (Cruaud et al 1990). Briefly, the antigen was dissolved in hexane (2µl/ml, 100ng/well) and incubated overnight at 37 o C. Blocked by the addition of 100µl of 5% bovine serum albumin (BSA) at 37°C for 2 hr, and washed with phosphate-buffered saline (PBS) pH 7.4.…”

“…In the last few years the characterization and identification of new specific antigens have restimulated the interest in the serologic diagnosis of TB (Daffé et al 1989, Laszlo et al 1992, Papa et al 1993). Among HIV seronegative TB patients, these serologic tests offer high sensitivity and specificity and may provide a wide clinical application (Daniel 1989, Wilkins & Ivanyi 1990, Cruaud et al 1990. But, their possible benefits for improved diagnosis of TB in HIV seropositive patients have not been determined (Theuer et al 1989, Hoeppner 1990, Berlie et al 1991.…”

Deciphering sulfoglycolipids of Mycobacterium tuberculosis

“…However, this compound was subsequently found to lack a sulfate ester group ( 9,10 ), requiring its classifi cation as a sulfatide to be revised ( 11 ). Nevertheless, the presence of di-acylated sulfolipids in the cell envelope of M. tuberculosis was defi nitively proven by three recent studies.…”

Serodiagnosis of Tuberculosis: Comparison of Immunoglobulin A (IgA) Response to Sulfolipid I with IgG and IgM Responses to 2,3-Diacyltrehalose, 2,3,6-Triacyltrehalose, and Cord Factor Antigens

“…They are 2,3-diacyltrehalose (DAT), 2,3,6-triacyltrehalose (TAT), 2,3,6,6Ј-tetraacyltrehalose 2Ј-sulfate (sulfolipid I [SL-I]), and trehalose-6,6Ј-dimycolate (cord factor [CF]). Using the enzyme-linked immunosorbent assay (ELISA) technique, several studies were performed with these antigens, and an extensive variability in immunoglobulin G (IgG) or IgM titers (6,7,11,18,20,(24)(25)(26) was obtained, i.e., test sensitivities were as different as 11 to 88% for the DAT antigen (11,25) and 51 to 93% for the TAT antigen (11,26). These contradictory results are due to the ELISA protocol used (the different antigen concentrations or the use of detergent in washing buffers) (15,30), or to the different sera groups analyzed (the number and type of patients and control subjects) (10).…”