Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test

Lucassen, Ralf; Schulte-Pelkum, Johannes; Csuvarszki, C.; Kleine-Tebbe, J.; Fooke, Margrit; Mähler, Michael

doi:10.1155/2010/524084

Cited by 11 publications

(9 citation statements)

References 7 publications

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“…The sIgE detection method we used was ALLERG-O-LIQ. According to the studies by various authors, the consistency of ALLERG-O-LIQ with ImmunoCAP results (the most common method used in allergy diagnosis) for quantitative or semi-quantitative determination of various allergens has been verified [ 22 - 24 ], although the correlation is stronger for aeroallergens than for food allergens.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Sun

Zheng

Zhang

et al. 2014

Multidiscip Respir Med

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BackgroundThe distribution of allergens may vary with different geographic areas, suggesting the importance of local epidemiological data to support evidence-based prevention and management of allergic diseases. We investigated the distribution of common allergens in allergic patients in Guangzhou, southern China.Methods7,047 patients with allergic symptoms were examined for serum sIgE to 15 common allergens in this region, based on the protocol of reversed enzyme allergosorbent test.Results4,869 (69.09%) of the subjects tested positive for sIgE to at least one of the 15 common allergens. There was no statistical difference in the overall rate of positive sIgE detection between males (3128/4523, 69.16%) and females (1741/2524, 68.98%). Der pteronyssinus and Der farinae were the most common aeroallergens, while eggs and cow’s milk the most common food allergens, responsible for higher positive rates of sIgE responses. A good correlation in positive sIgE response was found between Der pteronyssinus and Der farinae. By age-group analysis, we noted several peaks of sensitization to certain allergens: Der pteronyssinus, Der farinae, and Blomiatropicalis at age between 9 and 12; Blattellagermanica and mosquito at age between 15 and 18, cow’s milk before age 3; eggs and flour at age between 3 and 6; crabs and shrimps at age between 12 and 15. Along with older age, there was an ascending tendency in the overall positive rate of sIgE response to house dust mites among subjects who tested positive for sIgE to eggs or cow’s milk.ConclusionsDer pteronyssinus, Der farinae, cow’s milk, and eggs are major allergens in Guangzhou. Sensitization to eggs and cow’s milk is more common at younger age, and then gives place to the increasing prevalence of sensitization to Der pteronyssinus and Der farinae at older age. Such a sequence of events may be a result of allergy march. Knowledge on the prevalence of allergen sensitization in different age groups would help early diagnosis and intervention of allergic diseases in this large geographical region.

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Section: Discussionmentioning

confidence: 99%

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Sun

Zheng

Zhang

et al. 2014

Multidiscip Respir Med

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“…We used class 2 as the cut-off level of positive results for both ImmunoCAP and AdvanSure, as suggested by the manufacturers, in calculating the agreement percentage for positive and negative calls. The cut-off for a positive result from the GOLD chip was determined as the fluorescence intensity corresponding to 0.7 IU/mL of IgE for each allergen extract [ 26 27 28 ]. The overall total agreement rates were 92.2% for GOLD vs Immuno-CAP, 66.1% for ImmunoCAP vs AdvanSure, and 66.1% for GOLD vs AdvanSure ( Table 3 ).…”

Section: Resultsmentioning

confidence: 99%

Allergen Microarrays forIn VitroDiagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure

et al. 2018

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BackgroundIn vitro detection of the allergen-specific IgE antibody (sIgE) is a useful tool for the diagnosis and treatment of allergies. Although multiple simultaneous allergen tests offer simple and low-cost screening methods, these platforms also have limitations with respect to multiplexibility and analytical performance. As an alternative assay platform, we developed and validated a microarray using allergen extracts that we termed “GOLD” chip.MethodsSerum samples of 150 allergic rhinitis patients were used in the study, and the diagnostic performance of the microarray was compared with that of AdvanSure (LG Life Sciences, Daejun, Korea) and ImmunoCAP (Phadia, Uppsala, Sweden). Standard IgE samples were used for the quantitative measurement of sIgEs.ResultsThe microarray-based assay showed excellent performance in the quantitative measurement of sIgEs, demonstrating a linear correlation within the range of sIgE concentrations tested. The limit of detection (LOD) was lower than 0.35 IU/mL, which is the current standard for the LOD cut-off. The assay also provided highly reproducible sets of data. The total agreement percentage of positive and negative calls was 92.2% compared with ImmunoCAP. Moreover, an outstanding correlation was observed between the microarray and the ImmunoCAP results, with Cohen's kappa and Pearson correlation coefficient values of 0.80 and 0.79, respectively.ConclusionsThe microarray-based in vitro diagnostic platform offers a sensitive, reproducible, and highly quantitative method to detect sIgEs. The results showed strong correlations with that of ImmunoCAP. These results suggest that the new allergen microarray can serve as a useful alternative to current screening platforms, ultimately becoming a first-line screening method.

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“…LFIA is the combination of a labeled immunoassay with chromatography, in which capillary forces force the analyte to move. Specific recognition elements, representing specific binding moieties, are immobilized on the membrane surface and are thus able to detect different analytes, such as allergens [9,10]. LFIAs have several key features [11]: (1) reaction speed is fast, occurring in as little as a few minutes; (2) the assay can automatically separate target analytes from biological matrices without complex additional steps; and (3) the assay can be adapted to suit a variety of cluttered outdoor environments, without the need for highly skilled personnel to operate equipment or carry out complex analytical procedures.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis

2020

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The prevalence of allergic rhinitis (AR) is increasing worldwide. However, the current systems used to measure levels of immunoglobulin E (IgE) in sera are associated with several disadvantages that limit their further application. Consequently, there is a need to develop novel highly sensitive strategies that can rapidly detect IgE in a quantitative manner. The development of such systems will significantly enhance our ability to diagnose, treat, and even prevent AR. Herein, we describe our experience of using quantum dot-based lateral flow immunoassay (QD-LFIA), combined with a portable fluorescence immunoassay chip detector (PFICD), to detect serum-specific IgE against Dermatophagoides pteronyssinus (Der-p) and Dermatophagoides farinae (Der-f), two common mite allergens in China. Our data showed that our system could detect serum-specific levels of IgE against Der-p and Der-f as low as 0.093 IU/mL and 0.087 IU/mL, respectively. We also established a standard curve to determine serumspecific IgE concentrations that correlated well with the clinical BioIC microfluidics system. The sensitivity of our assay was 96.7% for Der-p and 95.5% for Der-f, while the specificity was 87.2% for Der-p and 85.3% for Der-f. Collectively, our results demonstrate that QD-LFIA is a reliable system that could be applied to detect serum-specific IgE in accordance with clinical demands. This QD-LFIA strategy can be applied at home, in hospitals, and in pharmacies, with reduced costs and time requirements when compared with existing techniques. In the future, this system could be developed to detect other types of allergens and in different types of samples (for example, whole blood).

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Evaluation of a Novel Rapid Test System for the Detection of Allergic Sensitization to Timothy Grass Pollen against Established Laboratory Methods and Skin Prick Test

Cited by 11 publications

References 7 publications

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Prevalence of allergen sensitization among patients with allergic diseases in Guangzhou, Southern China: a four-year observational study

Allergen Microarrays forIn VitroDiagnostics of Allergies: Comparison with ImmunoCAP and AdvanSure

A quantum dot-based lateral flow immunoassay for the rapid, quantitative, and sensitive detection of specific IgE for mite allergens in sera from patients with allergic rhinitis

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