Evaluation of a Physical-Chemical Protocol for Porcine Tracheal Decellularization

Guimarães, Aline; Correia, Aristides Tadeu; Alves, B.P.; Silva, R.S. Da; Martins, Johan; Pěgo‐Fernandes, Paulo M.; Xavier, Navarro; Dolhnikoff, Marisa; Cardoso, Paulo Francisco Guerreiro

doi:10.1016/j.transproceed.2019.01.042

Cited by 17 publications

(12 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“… [ 42 , 43 , [72] , [73] , [74] , [75] ] Require subsequent treatments for the removal of cellular contents Immersion and agitation Rupture tissues and cells, and isolate cells from basement membranes Alter ECM architecture. [ 42 , 50 , 65 , 66 , 70 , 72 ] Need optimal standards for mechanical force and exposure time Perfusion Remove cells from ECM and help the removal of cellular components and debris within the organ's natural vasculature Cannot process tissues without innate vasculature. [ 14 , [49] , [50] , [51] , [52] , 57 , [76] , [77] , [78] , [79] , [80] , [81] ] Sophisticated to operate the perfusion devices Scraping Mechanically remove cells from ECM surface Damage underlying basement membranes.…”

Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

See 1 more Smart Citation

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

360

263

View full text Add to dashboard Cite

The application of scaffolding materials is believed to hold enormous potential for tissue regeneration. Despite the widespread application and rapid advance of several tissue-engineered scaffolds such as natural and synthetic polymer-based scaffolds, they have limited repair capacity due to the difficulties in overcoming the immunogenicity, simulating in-vivo microenvironment, and performing mechanical or biochemical properties similar to native organs/tissues. Fortunately, the emergence of decellularized extracellular matrix (dECM) scaffolds provides an attractive way to overcome these hurdles, which mimic an optimal non-immune environment with native three-dimensional structures and various bioactive components. The consequent cell-seeded construct based on dECM scaffolds, especially stem cell-recellularized construct, is considered an ideal choice for regenerating functional organs/tissues. Herein, we review recent developments in dECM scaffolds and put forward perspectives accordingly, with particular focus on the concept and fabrication of decellularized scaffolds, as well as the application of decellularized scaffolds and their combinations with stem cells (recellularized scaffolds) in tissue engineering, including skin, bone, nerve, heart, along with lung, liver and kidney.

show abstract

Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

“…Third, compared to whole organ perfusion, this method is a more accessible and easily executed procedure [ 56 ]. However, compared with perfusion, using this method may cause more damages to tissues due to the limited diffusion of chemicals by agitation [ 70 , 71 ].…”

Section: Classification and Fabrication Of Decm Scaffoldsmentioning

confidence: 99%

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Zhang

Chen

Hong

et al. 2022

Bioactive Materials

360

263

View full text Add to dashboard Cite

show abstract

“…With this idea, different researchers have developed various strategies in order to minimize the decellularization time by including the use of enzymes that degrade the extracellular matrix such as trypsin, freezing and thawing, lyophilization, sonication, vacuum, or specific bioreactors in combination with detergents. 13,16,18,[20][21][22] Another strategy developed to ensure the availability of decellularized tracheas when necessary is the cryogenic preservation of the tracheas once decellularized. Cryopreservation would not only allow a stock of organs ready for transplantation, but it also has other additional advantages.…”

Section: Introductionmentioning

confidence: 99%

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

et al. 2021

View full text Add to dashboard Cite

Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from decellularized organs. Moreover, the long-term structural, ultrastructural, and biomechanical effect of cryopreservation of decellularized tracheas were also estimated. Results: Two percent SDS was the most effective detergent tested concerning cell removal and preservation of the histological and biomechanical properties of the tracheal wall. However, long-term cryopreservation had no an appreciable effect on the structure, ultrastructure, and biomechanics of decellularized tracheal rings. Conclusion: The results presented here reinforce the use of SDS as a valuable decellularizing agent for porcine tracheas. Furthermore, a cryogenic preservation protocol is described, which has minimal impact on the histological and biomechanical properties of decellularized porcine tracheas.

show abstract

“…Heatmap representing the proportion of publications by year that utilized specific translational research methodologies from construct characterization to human trial to investigate laryngeal tissue engineering 85‐87,91,92,94,96,97,198‐306 …”

Section: Discussionmentioning

confidence: 99%

Tissue engineering applications in otolaryngology—The state of translation

Niermeyer

Rodman

et al. 2020

Laryngoscope Investig Oto

View full text Add to dashboard Cite

While tissue engineering holds significant potential to address current limitations in reconstructive surgery of the head and neck, few constructs have made their way into routine clinical use. In this review, we aim to appraise the state of head and neck tissue engineering over the past five years, with a specific focus on otologic, nasal, craniofacial bone, and laryngotracheal applications. A comprehensive scoping search of the PubMed database was performed and over 2000 article hits were returned with 290 articles included in the final review. These publications have addressed the hallmark characteristics of tissue engineering (cellular source, scaffold, and growth signaling) for head and neck anatomical sites. While there have been promising reports of effective tissue engineered interventions in small groups of human patients, the majority of research remains constrained to in vitro and in vivo studies aimed at furthering the understanding of the biological processes involved in tissue engineering. Further, differences in functional and cosmetic properties of the ear, nose, airway, and craniofacial bone affect the emphasis of investigation at each site. While otolaryngologists currently play a role in tissue engineering translational research, continued multidisciplinary efforts will likely be required to push the state of translation towards tissue‐engineered constructs available for routine clinical use. Level of Evidence NA.

show abstract

Evaluation of a Physical-Chemical Protocol for Porcine Tracheal Decellularization

Cited by 17 publications

References 8 publications

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Decellularized extracellular matrix scaffolds: Recent trends and emerging strategies in tissue engineering

Optimization of a decellularization protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study

Tissue engineering applications in otolaryngology—The state of translation

Contact Info

Product

Resources

About