Evaluation of a Piezoelectric System as an Alternative to Electroencephalogram/ Electromyogram Recordings in Mouse Sleep Studies

Mang, Géraldine M.; Nicod, Jérôme; Emmenegger, Yann; Donohue, Kevin D.; O’Hara, Bruce F.; Franken, Paul

doi:10.5665/sleep.3936

Cited by 99 publications

(117 citation statements)

References 32 publications

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“…While electroencephalographic (EEG) recording offers insight into sleep quality and stage, the piezoelectric sleep monitoring system offers a non-invasive approach to only quantify wakefulness and sleep durations, concordant with EEG analysis 41 . It is unclear how TBI or resolvin treatment may independently alter respiratory rate and confound the algorithmic detection of sleep, but we have previously demonstrated that the piezoelectric sleep system detects discrete bouts of 3 Hz breathing classified as sleep following mFPI.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Resolvins AT-D1 and E1 differentially impact functional outcome, post-traumatic sleep, and microglial activation following diffuse brain injury in the mouse

Harrison¹,

Rowe²,

Ellis³

et al. 2015

Brain, Behavior, and Immunity

104

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Traumatic brain injury (TBI) is induced by mechanical forces which initiate a cascade of secondary injury processes, including inflammation. Therapies which resolve the inflammatory response may promote neural repair without exacerbating the primary injury. Specific derivatives of omega-3 fatty acids loosely grouped as specialized pro-resolving lipid mediators (SPMs) and termed resolvins promote the active resolution of inflammation. In the current study, we investigate the effect of two resolvin molecules, RvE1 and AT-RvD1, on post-traumatic sleep and functional outcome following diffuse TBI through modulation of the inflammatory response. Adult, male C57BL/6 mice were injured using a midline fluid percussion injury (mFPI) model (6-10 min righting reflex time for brain-injured mice). Experimental groups included mFPI administered RvE1 (100ng daily), AT-RvD1 (100ng daily), or vehicle (sterile saline) and counterbalanced with uninjured sham mice. Resolvins or saline were administered daily for seven consecutive days beginning 3 days prior to TBI to evaluate proof-of-principle to improve outcome. Immediately following diffuse TBI, post-traumatic sleep was recorded for 24 hours post-injury. For days 1-7 post-injury, motor outcome was assessed by Rotarod. Cognitive function was measured at 6 days post-injury using Novel Object Recognition (NOR). At 7 days post-injury, microglial activation was quantified using immunohistochemistry for Iba-1. In the diffuse brain-injured mouse, AT-RvD1 treatment, but not RvE1, mitigated motor and cognitive deficits. RvE1 treatment significantly increased post-traumatic sleep in brain-injured mice compared to all other groups. RvE1 treated mice displayed a higher proportion of ramified microglia and lower proportion of activated rod microglia in the cortex compared to saline or AT-RvD1 treated brain-injured mice. Thus, RvE1 treatment modulated post-traumatic sleep and the inflammatory response to TBI, albeit independently of improvement in motor and cognitive outcome as seen in AT-RvD1-treated mice. This suggests AT-RvD1 may impart functional benefit through mechanisms other than resolution of inflammation alone.

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Section: Discussionmentioning

confidence: 99%

“…The non-invasive sleep cage system (Signal Solutions, Lexington, KY) used in this study consisted of 16 separate units which simultaneously monitor sleep and wake states, as previously published 39-41 . Each cage unit housed a single mouse inside 18 × 18 centimeter walled compartments with attached food and water structures 39 .…”

Section: Methodsmentioning

confidence: 99%

Resolvins AT-D1 and E1 differentially impact functional outcome, post-traumatic sleep, and microglial activation following diffuse brain injury in the mouse

Harrison¹,

Rowe²,

Ellis³

et al. 2015

Brain, Behavior, and Immunity

104

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“…Piezo foil PVDF based circadian rhythm scoring of sleep/wake rhythm was used as established in Donohue et al (2008), and further used in AD studies (Duncan et al, 2012; Mang et al, 2014; Sethi et al, 2015). Mice were individually housed for 5 days in clean plastic cages (Signal Solutions LLC) with ~5 mm of corn cob bedding lining the floor and lined with the PVDF foil.…”

Section: Methodsmentioning

confidence: 99%

Fus1 KO Mouse As a Model of Oxidative Stress-Mediated Sporadic Alzheimer's Disease: Circadian Disruption and Long-Term Spatial and Olfactory Memory Impairments

Coronas‐Samano

Baker

Tan

et al. 2016

Front. Aging Neurosci.

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Insufficient advances in the development of effective therapeutic treatments of sporadic Alzheimer's Disease (sAD) to date are largely due to the lack of sAD-relevant animal models. While the vast majority of models do recapitulate AD's hallmarks of plaques and tangles by virtue of tau and/or beta amyloid overexpression, these models do not reflect the fact that in sAD (unlike familial AD) these genes are not risk factors per se and that other mechanisms like oxidative stress, metabolic dysregulation and inflammation play key roles in AD etiology. Here we characterize and propose the Fus1 KO mice that lack a mitochondrial protein Fus1/Tusc2 as a new sAD model. To establish sAD relevance, we assessed sAD related deficits in Fus1 KO and WT adult mice of 4–5 months old, the equivalent human age when the earliest cognitive and olfactory sAD symptoms arise. Fus1 KO mice showed oxidative stress (increased levels of ROS, decreased levels of PRDX1), disruption of metabolic homeostasis (decreased levels of ACC2, increased phosphorylation of AMPK), autophagy (decreased levels of LC3-II), PKC (decreased levels of RACK1) and calcium signaling (decreased levels of Calb2) in the olfactory bulb and/or hippocampus. Mice were behaviorally tested using objective and accurate video tracking (Noldus), in which Fus1 KO mice showed clear deficits in olfactory memory (decreased habituation/cross-habituation in the short and long term), olfactory guided navigation memory (inability to reduce their latency to find the hidden cookie), spatial memory (learning impairments on finding the platform in the Morris water maze) and showed more sleep time during the diurnal cycle. Fus1 KO mice did not show clear deficits in olfactory perception (cross-habituation), association memory (passive avoidance) or in species-typical behavior (nest building) and no increased anxiety (open field, light-dark box) or depression/anhedonia (sucrose preference) at this relatively young age. These neurobehavioral deficits of the Fus1 KO mice at this relatively young age are highly relevant to sAD, making them suitable for effective research on pharmacological targets in the context of early intervention of sAD.

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“…The piezo system (Signal Solutions, LLC, Lexington, Kentucky, USA) is described in detail elsewhere (Donohue et al 2008; Mang et al 2014). Briefly, the piezo sensor is a thin dielectric sheet that generates a voltage signal in response to changes in surface pressure.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting quasirhythmic “piezo” signal differentiates sleep from quiet or active wakefulness with accuracy comparable to a human observer (Donohue et al 2008). Mang et al (2014) found that the decision statistic used by the same classifier to distinguish sleep from wakefulness (Donohue et al 2008) appears to change in value following REM - NREM transitions as well. Sato et al (2010) used a piezoelectric transducer to monitor mice and documented rapid increases in breathing rate during sleep with atonic posture, presumably in REM .…”

Section: Introductionmentioning

confidence: 99%

Noninvasive dissection of mouse sleep using a piezoelectric motion sensor

Yaghouby

Donohue

O’Hara

et al. 2016

Journal of Neuroscience Methods

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Background Changes in autonomic control cause regular breathing during NREM sleep to fluctuate during REM. Piezoelectric cage-floor sensors have been used to successfully discriminate sleep and wake states in mice based on signal features related to respiration and other movements. This study presents a classifier for noninvasively classifying REM and NREM using a piezoelectric sensor. New Method Vigilance state was scored manually in 4-second epochs for 24-hour EEG/EMG recordings in twenty mice. An unsupervised classifier clustered piezoelectric signal features quantifying movement and respiration into three states: one active; and two inactive with regular and irregular breathing respectively. These states were hypothesized to correspond to Wake, NREM, and REM respectively. States predicted by the classifier were compared against manual EEG/EMG scores to test this hypothesis. Results Using only piezoelectric signal features, an unsupervised classifier distinguished Wake with high (89% sensitivity, 96% specificity) and REM with moderate (73% sensitivity, 75% specificity) accuracy, but NREM with poor sensitivity (51%) and high specificity (96%). The classifier sometimes confused light NREM sleep—characterized by irregular breathing and moderate delta EEG power—with REM. A supervised classifier improved sensitivities to 90, 81, and 67% and all specificities to over 90% for Wake, NREM, and REM respectively. Comparison with Existing Methods Unlike most actigraphic techniques, which only differentiate sleep from wake, the proposed piezoelectric method further dissects sleep based on breathing regularity into states strongly correlated with REM and NREM. Conclusions This approach could facilitate large-sample screening for genes influencing different sleep traits, besides drug studies or other manipulations.

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Evaluation of a Piezoelectric System as an Alternative to Electroencephalogram/ Electromyogram Recordings in Mouse Sleep Studies

Abstract: Mang GM, Nicod J, Emmenegger Y, Donohue KD, O'Hara BF, Franken P. Evaluation of a piezoelectric system as an alternative to electroencephalogram/electromyogram recordings in mouse sleep studies.

Cited by 99 publications

References 32 publications

Resolvins AT-D1 and E1 differentially impact functional outcome, post-traumatic sleep, and microglial activation following diffuse brain injury in the mouse

Resolvins AT-D1 and E1 differentially impact functional outcome, post-traumatic sleep, and microglial activation following diffuse brain injury in the mouse

Fus1 KO Mouse As a Model of Oxidative Stress-Mediated Sporadic Alzheimer's Disease: Circadian Disruption and Long-Term Spatial and Olfactory Memory Impairments

Noninvasive dissection of mouse sleep using a piezoelectric motion sensor

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