Evaluation of a potassium chloride extract of Brucella abortus in an ELISA for detecting Brucella antibodies in bulk tank milk samples from cows

Thoen, Charles O.; Haas, Cathy A.; Angus, R D; Townsend, Angela S.

doi:10.1016/0378-1135(94)00120-l

Veterinary Microbiology

1995

DOI: 10.1016/0378-1135(94)00120-l

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Evaluation of a potassium chloride extract of Brucella abortus in an ELISA for detecting Brucella antibodies in bulk tank milk samples from cows

Charles O. Thoen

Cathy A. Haas

R D Angus

et al.

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2001

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Cited by 5 publications

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“…An economic analysis of the control strategies for bovine TB surveillance indicated that enzyme-linked immunosorbent assay (ELISA) testing of bulk milk samples may be a cost-effective strategy if the testing became feasible (7). Encouraging results for the detection of antibodies to M. bovis in individual and bulk milk samples were recently reported (6,8), and the detection of antibodies in bulk milk samples has been used in control programs for the diagnosis of brucellosis, enzootic bovine leukosis, and Johne's disease in cattle (9)(10)(11). However, one of the concerns with the use of serological tests for the detection of M. bovis infection in cattle has been the variation in the sensitivities of tests when applied to sera from M. bovis-infected cattle from different countries.…”

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confidence: 99%

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Buddle

Wilson

Luo

et al. 2013

Clin Vaccine Immunol

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Milk samples from dairy cows provide a ready source of material for measuring antibody responses to Mycobacterium bovis antigens. In this study, we evaluated the IDEXX enzyme-linked immunosorbent assay (ELISA) for the measurement of antibody responses to M. bovis antigens MPB70 and MPB83 in milk samples from New Zealand cattle. Test sensitivities for individual milk and serum samples were assessed in samples collected from 44 M. bovis-infected cows, and test specificities were assessed in milk samples collected from 356 cows from tuberculosis (TB)-free herds. Milk vat samples were collected from 505 herds from regions with relatively high or low prevalences of infection. The ELISA had a sensitivity of 50% and a specificity of 97.5% for milk samples, and the test sensitivities for milk and serum samples were the same. Dilution of the positive test milk samples in milk from noninfected cows at 1/10, 1/20, and 1/50 dilutions reduced the proportions of positive responses to 13/21, 9/21, and 4/21, respectively. Small differences were observed in the ELISA responses of milk samples from individual TB-free cows collected at different times during lactation. No significant differences were detected in the ELISA responses of milk vat samples collected from infected and noninfected herds. This study shows that milk samples can be substituted for serum samples for screening individual cows for M. bovis infection, and pooling of milk samples from 10 to 20 animals can result in a reduction in the sensitivity by approximately 50%. However, screening of milk vat samples is unlikely to be useful in countries with low prevalences of M. bovis in cattle and large herd sizes. Bovine tuberculosis (TB) caused by Mycobacterium bovis is a major economic problem in many countries, and control of this chronic disease in domestic livestock has often proved difficult. Although the disease has been eradicated from cattle in a number of countries through the implementation of a "test-andslaughter" control program, this strategy has been less effective in countries which have a wildlife reservoir of M. bovis infection and has been impractical in countries where the strategy is unaffordable or socially unacceptable (1). The test-and-slaughter programs have been based on screening of animals using the tuberculin skin test, which measures delayed hypersensitivity responses to purified protein derivative (PPD) prepared from M. bovis or which, in some countries, compares PPDs prepared from M. bovis and Mycobacterium avium. More recently, the whole-blood gamma interferon (IFN-␥) test has been used in conjunction with the skin test to improve specificities by retesting skin test reactor cattle or to improve sensitivities by retesting skin test-negative animals from chronically infected herds (2, 3). Although the IFN-␥ test has made a valuable contribution in improving the accuracy of diagnosing TB in cattle, additional types of assays are needed, particularly assays which are inexpensive and which can potentially be used on pooled samples from group...

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Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Buddle

Wilson

Luo

et al. 2013

Clin Vaccine Immunol

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“…The use of tests adapted for use with bulk milk samples is of interest because of the potential cost savings and the possibility for the automation of testing. Tests adapted for use with bulk milk have been developed for several bovine diseases, such as those caused by Brucella abortus (22), bovine leukemia virus (19), bovine viral diarrhea virus (15), Leptospira hardjo (1), bovine herpesvirus type 1 (24), Mycobacterium avium subsp. paratuberculosis (14), bovine corona virus (23), bovine respiratory syncytial virus (2), Coxiella burnetii (16), Ostertagia spp.…”

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confidence: 99%

Evaluation of Two Enzyme-Linked Immunosorbent Assays for DetectingSalmonella entericasubsp.entericaSerovar Dublin Antibodies in Bulk Milk

Veling

Zijderveld²,

Bemmel³

et al. 2001

Clin Diagn Lab Immunol

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Two enzyme-linked immunosorbent assays (ELISAs) for the detecting Salmonella enterica subsp. enterica serovar Dublin antibodies in bulk milk were developed and evaluated for potential use in control programs. The ELISAs were based on either lipopolysacharide (LPS ELISA) or flagellar antigen (GP ELISA). Sensitivity was determined with 79 case herds with a wide range of clinical signs. Specificity was determined with 125 Dutch and 200 Swedish control herds. The relation between antibodies in bulk milk, antibodies in serum, and the level of milk production of individual cows was studied with 61 case herds. The optimal optical density (OD) values of the LPS ELISA and the GP ELISA were determined to be 0.2 and 0.5, respectively. The sensitivities of the LPS ELISA and the GP ELISA were 54 and 63%, respectively, with a specificity of 98% for both ELISAs with samples from the Dutch control herds. The specificities for samples from the Swedish herds were 100% for the LPS ELISA and 95% for the GP ELISA. The sensitivity of the combination of tests was 65% when samples were run in parallel, and the specificity was 100% when samples were run in series, irrespective of whether the samples came from Dutch or Swedish control herds. The variance (R 2 ) in the OD value for bulk milk samples could be explained by the percentage of seropositive lactating cows in a herd with the LPS ELISA for 51% of the samples and with the GP ELISA for 72%. The variance in the OD value was best explained by the combination of the percentage of seropositive lactating cows in the herd and the mean log 10 serum antibody titer for that herd (R 2 ‫؍‬ 62% for the LPS ELISA and R 2 ‫؍‬ 75% for the GP ELISA). Case herds more often tested negative by the ELISA with bulk milk when the percentage of seropositive lactating cows was less than 5%. It is concluded that both ELISAs with bulk milk can be used in control programs to distinguish between infected and noninfected herds. Specificity can be increased by using the two tests in combination. Sensitivity was relatively low for both single tests and both tests combined.

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Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea

Jeon

Kim

et al. 2010

Research in Veterinary Science

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Evaluation of a potassium chloride extract of Brucella abortus in an ELISA for detecting Brucella antibodies in bulk tank milk samples from cows

Cited by 5 publications

References 3 publications

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Evaluation of a Commercial Enzyme-Linked Immunosorbent Assay for the Diagnosis of Bovine Tuberculosis from Milk Samples from Dairy Cows

Evaluation of Two Enzyme-Linked Immunosorbent Assays for DetectingSalmonella entericasubsp.entericaSerovar Dublin Antibodies in Bulk Milk

Evaluation of enzyme-linked immunosorbent assay using milk samples as a potential screening test of bovine tuberculosis of dairy cows in Korea

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