Evaluation of a preclinical photon-counting CT prototype for pulmonary imaging

Kopp, Felix K.; Daerr, Heiner; Si‐Mohamed, Salim; Sauter, Andreas; Ehn, Sebastian; Fingerle, Alexander A.; Brendel, Bernhard; Pfeiffer, Franz; Roessl, Ewald; Rummeny, Ernst J.; Pfeiffer, Daniela; Proksa, Roland; Douek, Philippe; Noël, Peter B.

doi:10.1038/s41598-018-35888-1

Cited by 61 publications

(45 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This next generation spectral photon counting CT (SPCCT) is expected to further expand the boundaries of spectral CT in terms of noise reduction and tissue differentiation (14). It also improves the intrinsic spatial resolution owing to small pixel detector size (~250 µm) (15,16). In addition, SPCCT allows selective visualization and quantification of multiple contrast agents by exploiting the K-edge discontinuity in X-ray absorption, a concept coined 'K-edge imaging' or 'multicolor CT' (17).…”

Section: Ivyspring International Publishermentioning

confidence: 99%

Multicolor spectral photon counting CT monitors and quantifies therapeutic cells and their encapsulating scaffold in a model of brain damage

Cuccione¹,

Chhour²,

Dumot³

et al. 2020

Nanotheranostics

Self Cite

View full text Add to dashboard Cite

Rationale & aim: Various types of cell therapies are currently under investigation for the treatment of ischemic stroke patients. To bridge the gap between cell administration and therapeutic outcome, there is a need for non-invasive monitoring of these innovative therapeutic approaches. Spectral photon counting computed tomography (SPCCT) is a new imaging modality that may be suitable for cell tracking. SPCCT is the next generation of clinical CT that allows the selective visualization and quantification of multiple contrast agents. The aims of this study are: (i) to demonstrate the feasibility of using SPCCT to longitudinally monitor and quantify therapeutic cells, i.e. bone marrow-derived M2-polarized macrophages transplanted in rats with brain damage; and (ii) to evaluate the potential of this approach to discriminate M2-polarized macrophages from their encapsulating scaffold. Methods: Twenty one rats received an intralesional transplantation of bone marrow-derived M2-polarized macrophages. In the first set of experiments, cells were labeled with gold nanoparticles and tracked for up to two weeks post-injection in a monocolor study via gold K-edge imaging. In the second set of experiments, the same protocol was repeated for a bicolor study, in which the labeled cells are embedded in iodine nanoparticle-labeled scaffold. The amount of gold in the brain was longitudinally quantified using gold K-edge images reconstructed from SPCCT acquisition. Animals were sacrificed at different time points post-injection, and ICP-OES was used to validate the accuracy of gold quantification from SPCCT imaging. Results: The feasibility of therapeutic cell tracking was successfully demonstrated in brain-damaged rats with SPCCT imaging. The imaging modality enabled cell monitoring for up to 2 weeks post-injection, in a specific and quantitative manner. Differentiation of labeled cells and their embedding scaffold was also feasible with SPCCT imaging, with a detection limit as low as 5,000 cells in a voxel of 250 × 250 × 250 µm in dimension in vivo. Conclusion: Multicolor SPCCT is an innovative translational imaging tool that allows monitoring and quantification of therapeutic cells and their encapsulating scaffold transplanted in the damaged rat brain.

show abstract

Section: Ivyspring International Publishermentioning

confidence: 99%

Multicolor spectral photon counting CT monitors and quantifies therapeutic cells and their encapsulating scaffold in a model of brain damage

Cuccione¹,

Chhour²,

Dumot³

et al. 2020

Nanotheranostics

Self Cite

View full text Add to dashboard Cite

show abstract

“…The photon-counting detector has five energy thresholds. A lung phantom including nodules of different sizes and shapes was scanned with this system and a conventional CT in standard and high-resolution modes, demonstrating the potential of photon-counting CT to improve the assessment of lung structures due to higher resolution compared to conventional CT [23]. Improved visualization of the in-stent lumen and in-stent re-stenosis in ten different coronary stents placed in a vessel phantom and filled with contrast agent was demonstrated in [3].…”

Section: Pre-clinical Evaluation Of Photon-counting Ctmentioning

confidence: 99%

“…A pre-clinical single-source CT system with photoncounting detector based on CZT (Philips Healthcare, Haifa, Israel) was evaluated both with phantoms and with animal scans. The system provides an in-plane field of view of 168 mm and a z coverage of 2.5 mm at the iso-center, with a rotation time of 1 s [23]. The size of the detector pixels is 0.5 × 0.5 mm 2 .…”

Section: Pre-clinical Evaluation Of Photon-counting Ctmentioning

confidence: 99%

Basic principles and clinical potential of photon-counting detector CT

et al. 2020

View full text Add to dashboard Cite

Photon-counting detectors are a new technology for future computed tomography (CT) systems, with the potential to overcome major limitations of conventional CT detectors. They provide energy-resolved CT data at very high spatial resolution without electronic noise. This review article gives an overview of the basic principles of photon-counting detector CT, of potential benefits and limitations, and of the clinical experience gained so far in pre-clinical installations.

show abstract

“…Therefore, 2 energy bins were set at 50.2 keV (51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64) to coincide with the K-edge energy of gadolinium, the extra energy bins were set to better managing the photon count rate (64-72, 72-85, 85-120). Further technical details are provided in previous studies 33,42 .…”

Section: Spectral Photon Counting Computed Tomography (Spcct) the Spmentioning

confidence: 99%

Spectral photon-counting CT imaging of colorectal peritoneal metastases: initial experience in rats

Thivolet

Bonnot

Blanchet

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

computed tomography imaging plays a major role in the preoperative assessment of tumor burden by providing an accurate mapping of the distribution of peritoneal metastases (PM). Spectral Photon Counting Computed Tomography (SPCCT) is an innovative imaging modality that could overcome the current limitations of conventional CT, offering not only better spatial resolution but also better contrast resolution by allowing the discrimination of multiple contrast agents. Based on this capability, we tested the feasibility of SPCCT in the detection of PM at different time of tumor growth in 16 rats inoculated with CC531 cells using dual-contrast injection protocols in two compartments (i.e. intravenous iodine and intraperitoneal gadolinium or the reverse protocol), compared to surgery. For all peritoneal regions and for both protocols, sensitivity was 69%, specificity was 100% and accuracy was 80%, and the correlation with surgical exploration was strong (p = 0.97; p = 0.0001). No significant difference was found in terms of diagnostic performance, quality of peritoneal opacification or diagnostic quality between the 2 injection protocols. We also showed poor vascularization of peritoneal metastases by measuring low concentrations of contrast agent in the largest lesions using SPCCT, which was confirmed by immunohistochemical analyses. In conclusion, SPCCT using dualcontrast agent injection protocols in 2 compartments is a promising imaging modality to assess the extent of PM in a rat model. Peritoneal metastases (PM) are part of the natural history of most abdominal and gynecological malignancies and have long been considered as a terminal disease. Over the past decades, the development of cytoreductive surgery with or without intraoperative hyperthermal intraperitoneal chemotherapy (HIPEC) has led to an improvement in survival of selected patients with resectable disease 1-4. For colorectal cancer, this strategy has significantly improved oncology outcomes 4,5. The completeness of the cytoreductive surgery is a crucial endpoint and is directly associated with the extent and distribution of PM in the peritoneal cavity 6,7. However, this aggressive treatment is associated with substantial morbidity (30-40%) and a mortality rate that is reported to range from 0-10%, resulting in a further drastic selection of patients in the pre-treatment work-up 8,9. In this context, preoperative evaluation of the peritoneal tumor burden, although extremely challenging, is essential for the selection of eligible patients for curative treatment 10,11. Computed tomography (CT) remains the reference imaging modality to assess the extent of PM. Although magnetic resonance imaging (MRI) and positron emission tomography-CT (PET-CT) have good diagnostic performance in some PM etiologies, these modalities are mainly used as a complement to CT evaluation 10,12-17. However, even though CT imaging offers high spatial resolution and fast acquisition time, its main drawback is its lack of contrast resolution, leading to a significant underestimation ...

show abstract

Evaluation of a preclinical photon-counting CT prototype for pulmonary imaging

Cited by 61 publications

References 42 publications

Multicolor spectral photon counting CT monitors and quantifies therapeutic cells and their encapsulating scaffold in a model of brain damage

Multicolor spectral photon counting CT monitors and quantifies therapeutic cells and their encapsulating scaffold in a model of brain damage

Basic principles and clinical potential of photon-counting detector CT

Spectral photon-counting CT imaging of colorectal peritoneal metastases: initial experience in rats

Contact Info

Product

Resources

About