Evaluation of a QuEChERS-like extraction approach for the determination of PBDEs in mussels by immuno-assay-based screening methods

Romanelli, Sara; Bettazzi, Francesca; Martellini, Tania; Shelver, Weilin L.; Cincinelli, Alessandra; Galarini, Roberta; Palchetti, Ilaria

doi:10.1016/j.talanta.2017.04.027

Cited by 7 publications

(3 citation statements)

References 26 publications

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“…Total concentrations of PBDE and 9 congeners (183, 85, 153, 154, 99, 100, 47, 66 and 28) were measured in muscle of males and females of Atlantic herring ( Clupea harengus ) by an accredited laboratory (EN ISO 17025; EN ISO 14001) through solvent extraction (acetonitrile) using a modified QuEChERS technique (Romanelli et al 2017 ), followed by analysis by GC-QQQ after purification. In this method, a pre-prepared sample is extracted in acetonitrile.…”

Section: Methodsmentioning

confidence: 99%

Bioaccumulation of different congeners of Poly-Brominated Diphenyl Ethers (PBDEs) in muscle tissue of males and females of Clupea harengus from the North Sea

Gaion

Morgan

Collier

et al. 2021

Environ Sci Pollut Res

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In the last decades, high concentrations of flame retardants (PBDE) in marine organisms have caused increasing concern among scientists with regards to their biomagnification potential and to human health. Poly-Brominated Diphenyl Ethers have been widely used in the past as flame retardants in various industrial sectors, and their presence in the biota has been associated with different toxicological effects. In this study, concentrations of 9 congeners of PBDE (183, 85, 153, 154, 99, 100, 47, 66 and 28) and morphometric parameters (Total Length, TL; Fork Length, FL in cm and weight) have been measured in muscle of males and females of Atlantic herring (Clupea harengus). Results showed no statistical differences between the concentrations of most of the congeners analysed between the two sexes, except for PBDE 153 (Mean ± standard error in males = 0.034 ± 0.005 μg/kg and in females = 0.086 ± 0.040 μg/kg; p = 0.003). This research contributes to better comprehend the ecotoxicological properties of these molecules and their implications for human consumption.

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Section: Methodsmentioning

confidence: 99%

Bioaccumulation of different congeners of Poly-Brominated Diphenyl Ethers (PBDEs) in muscle tissue of males and females of Clupea harengus from the North Sea

Gaion

Morgan

Collier

et al. 2021

Environ Sci Pollut Res

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“…In these cases, the transducer is used only for the measurement. This approach is generally performed with electrochemical transducers [54,55,56]. Immunomagnetic assays are believed to be particularly useful for the improvement of the analytical performance of the electrochemical immunoassays, since the immunoreaction is performed on a different surface, with respect to electrochemical detection.…”

Section: Immunosensor Development: Practical Tips and New Trendsmentioning

confidence: 99%

Trends and Perspectives in Immunosensors for Determination of Currently-Used Pesticides: The Case of Glyphosate, Organophosphates, and Neonicotinoids

et al. 2019

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Pesticides, due to their intensive use and their peculiar chemical features, can persist in the environment and enter the trophic chain, thus representing an environmental risk for the ecosystems and human health. Although there are several robust and reliable standard analytical techniques for their monitoring, the high frequency of contamination caused by pesticides requires methods for massive monitoring campaigns that are capable of rapidly detecting these compounds in many samples of different origin. Immunosensors represent a potential tool for simple, rapid, and sensitive monitoring of pesticides. Antibodies coupled to electrochemical or optical transducers have resulted in effective detection devices. In this review, the new trends in immunosensor development and the application of immunosensors for the detection of pesticides of environmental concern—such as glyphosate, organophosphates, and neonicotinoids—are described.

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“…Thus, we have optimized a competitive immunoassay procedure based on anti-glyphosate-antibody-modified magnetic beads. Immunomagnetic assays are claimed to be particularly useful for improving the analytical performance of electrochemical immunoassays, since the immunoreaction is performed on a different surface, with respect to electrochemical detection [ 23 , 24 ]. Competition has been obtained using a Horseradish Peroxidase (HRP) conjugated tracer.…”

Section: Introductionmentioning

confidence: 99%

Glyphosate Determination by Coupling an Immuno-Magnetic Assay with Electrochemical Sensors

Bettazzi

Romero-Natale

Torres

et al. 2018

Sensors

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Glyphosate (N-(phosphonomethyl)glycine) is the most frequently used broad-spectrum herbicide worldwide. Its mechanism of action is based on the inhibition of an enzyme that is essential to plant growth. Its intensive use has caused global contamination to occur, which has not only affected the ecosystems, but even food and other objects of common use. Thus, there is a pronounced need for developing analytical methods for glyphosate determination in different matrices. Here, an electrochemical competitive immunoassay, based on the use of antibody-modified magnetic particles, has been developed. Tetramethylbenzidine (TMB) has been used as an enzymatic substrate. The extent of the affinity reaction has been achieved by monitoring the current value, due to the reduction of the enzymatic product. A disposable screen-printed electrochemical cell has been used. The calibration curve has been recorded in the 0–10,000 ng/L concentration range, with a detection limit of 5 ng/L and quantification limit of 30 ng/L. The electrochemical immunoassay has also been applied to the analysis of spiked beer samples.

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Evaluation of a QuEChERS-like extraction approach for the determination of PBDEs in mussels by immuno-assay-based screening methods

Cited by 7 publications

References 26 publications

Bioaccumulation of different congeners of Poly-Brominated Diphenyl Ethers (PBDEs) in muscle tissue of males and females of Clupea harengus from the North Sea

Bioaccumulation of different congeners of Poly-Brominated Diphenyl Ethers (PBDEs) in muscle tissue of males and females of Clupea harengus from the North Sea

Trends and Perspectives in Immunosensors for Determination of Currently-Used Pesticides: The Case of Glyphosate, Organophosphates, and Neonicotinoids

Glyphosate Determination by Coupling an Immuno-Magnetic Assay with Electrochemical Sensors

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