Evaluation of a rapid coliform detection kit from clinical mastitis milk using colloidal gold nanoparticle–based immunochromatographic strips

Abstract

The accurate identification of mastitis‐causing bacteria assists in effective management by both dairy farmers and veterinarians and can be used to implement the selective use of antimicrobials for treatment. The purpose of this study was to evaluate the ability of our developed anti–ribosomal protein-L7/L12 antibody–coated immunochromatographic strip (ICS) test to detect coliforms in milk by comparing the results with the bacteriological culture method. We investigated the performance of the ICS test as compared with the bacteriological culture method using 308 milk samples from clinical bovine mastitis. First, to determine the optimal ICS test cutoff point for detecting coliform mastitis, we developed a receiver-operating characteristic curve. The result showed that the cutoff point was at 0.5 of our index. The sensitivity, specificity, positive predictive value (PPV), and negative predictive value of the ICS test were 81.3%, 84.8%, 69.2%, and 91.54%, respectively. As the clinical signs increased in severity, the F-measure, a weighted harmonic mean of the sensitivity and overall PPV performance, increased. Because it is especially important to treat clinical mastitis appropriately in the early stages of detection, the ICS test, which can be used by both dairy farmers and veterinarians on dairy farms, is considered to be a useful tool for detecting coliform mastitis, which often presents with severe signs.

Performance evaluation of a rapid immunochromatographic test kit in detecting bovine mastitis-causing streptococci

“…Moreover, we have developed test kits for Staph. aureus and coliforms using a Lateral flow assay [ 11 , 19 ]. The use of a test kit for streptococci combined with test kits for Staph.…”

Noodzakelijke stappen naar een daling van het antibioticumgebruik op de Vlaamse melkveebedrijven - Deel 3: sneltesten voor kiemdetectie

“…Er zijn tal van commerciële sneltesten voor kiemdetectie voorhanden. Deze testen kunnen zowel uit specifieke agars bestaan, als uit een Petrifilm™, welletjes, microchips of een PCR--test (Ganda et al, 2016;Kiku et al, 2021;Leimbach en Krömker, 2018a;Mansion-de Vries et al, 2014a;Royster et al, 2014). Bij sommige testen wordt zelfs terzelfder tijd de antimicrobiële gevoeligheid van de geïdentificeerde kiem bepaald, hetgeen kan helpen bij de keuze van de meest geschikte behandeling (Bates et al, 2020;Jones et al, 2019).…”

Is AMR in Dairy Products a Threat to Human Health? An Updated Review on the Origin, Prevention, Treatment, and Economic Impacts of Subclinical Mastitis

“… [ 192 ] Automatic digital diagnostics Automated milk leucocyte differential test (MLD) (Qscout) [ 193 ] Immunochromatographic strips The development of a nanoparticle-based–coated immunochromatographic strip (ICS) that accurately identifies mastitis-causing bacteria helps in successful management by both dairy farmers and veterinarians and can be utilized to implement the selective use of antimicrobials for therapy. [ 194 , 195 ] Infra-red thermography IRT is a simple, convenient, and portable diagnostic technique for diagnosing subclinical mastitis. [ 196 , 197 ] Sensor-based mastitis detection systems The proteolytic activity of plasmin as a biomarker was used to develop a magnetic nanoparticles-based colorimetric biosensor assay that was sensitive enough to detect modest levels (1ng/mL) of plasmin present in vitro in milk samples.…”