Evaluation of a rapid diagnostic test for the detection of Streptococcus pyogenes in invasive infections

Reijtman, Vanesa; Garcia, Maria; Mastroianni, Alejandra; Isasmendi, Adela; Pinheiro, José Luís Palmeiro; Pérez, Guadalupe; Hernández, C

doi:10.1016/j.ram.2019.08.004

Cited by 5 publications

(4 citation statements)

References 11 publications

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“…Due to the production of numerous virulence factors (M protein, pyrogenic exotoxins, haemolysins) they are responsible for recurrent strep throat, scarlet fever, myositis and fasciitis, endocarditis, arthritis, and ocular mucous membrane inflammation. This is a strictly human pathogen, associated with causing 500,000 deaths annually worldwide [19,20]. In the presented case, the patient was probably a carrier of bacteria belonging to this species.…”

Section: Discussionmentioning

confidence: 77%

<i>Candida glabrata</i> as an aetiological factor of the fulminant course of panophthalmitis

Bilska-Stokłosa¹,

Hampelska²,

Osmola³

et al. 2020

Ann Agric Environ Med.

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Introduction. The role of fungi in infections in immunocompromised patients is a growing problem in both diagnosis and treatment. Candida species are the most common cause of fungal, endogenous endophthalmitis and infections of the cornea. Case study. A patient was admitted to hospital due to acute inflammation of the tissue of the left orbit, 1.5 years after the corneal penetrating transplantation of the left eye with intracapsular extraction of lens and simultaneous anterior vitrectomy. The microbiological system identified: Streptococcus pyogenes, Staphylococcus aureus, and Candida glabrata in the patient. Conclusions.The factors conducive to fungal infections are: patient's old age, immune disorders and diabetes, as well as the presence of a necrotic tissue or a foreign body. All these parameters were met in this case. Only antibiotic therapy and long-term antifungal therapy, together with surgical debridement of the site of the ongoing infection produces clinical effects in such severe cases.

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Section: Discussionmentioning

confidence: 77%

<i>Candida glabrata</i> as an aetiological factor of the fulminant course of panophthalmitis

Bilska-Stokłosa¹,

Hampelska²,

Osmola³

et al. 2020

Ann Agric Environ Med.

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“…Although the design of the present study is not the same, rapid antigen tests that are developed with immunochromatographic methods for the rapid diagnosis of S. pyogenes are widely used for diagnostic purposes [ 36 , 37 , 38 ]. In their study, Reijtman et al attempted to develop an immunochromatographic method to detect S. pyogenes antigen directly from clinical samples and positive blood culture bottles.…”

Section: Discussionmentioning

confidence: 99%

“…In their study, Reijtman et al attempted to develop an immunochromatographic method to detect S. pyogenes antigen directly from clinical samples and positive blood culture bottles. They reported the sensitivity of the test they developed as 97.1% and the specificity as 97.8% and argued that they obtained reliable results in less than 10 min [ 36 ]. GAS is known to be among the most common bacterial pathogens, especially in children who are older than 3 years, and the incidence of GAS infections has increased in invasive and non-invasive forms in recent years.…”

Section: Discussionmentioning

confidence: 99%

Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

Kirkoyun Uysal,

Eryildiz,

Demirci

2024

Micromachines

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New rapid, reliable, and cost-effective alternative systems are needed for the rapid diagnosis of Streptococcus pyogenes. The aim of this study was to fabricate a microfluidic test device to detect Streptococcus pyogenes by combining the Loop-mediated isothermal amplification method via a 3D printer. Microfluidic test devices were designed in CATIA V5 Release 16 software, and data were directly transferred to a 3D printer and produced using the FDM method with biocompatible PLA filament. The S. pyogenes ATCC 19615 and different ATCC strains was used. Following identification by classical culture methods, a 0.5 McFarland suspension was prepared from the colonies, and DNA isolation was performed from this liquid by a boiling method. S. pyogenes specific speB gene was used to desing LAMP primer sets in PrimerExplorer V5 software and tested on a microfluidic device. LAMP reactions were performed on microfluidic device and on a microcentrifuge tube separately. Both results were analyzed using the culture method as the standard method to diagnostic values. Melting curve analysis of the amplicons of the LAMP reactions performed on a LightCycler 480 system to detect amplification. Among the 50 positive and 100 negative samples, only four samples were found to be false negative by LAMP reaction in a microcentrifuge tube, while eight samples were found to be false negative by LAMP reaction on a microfluidic device. Six samples were found to be false positive by the LAMP reaction in the microcentrifuge tube, while ten samples were found to be false positive by the LAMP reaction on a microfluidic chip. The sensitivity, specificity, positive predictive value, and negative predictive value of the LAMP reactions performed in the microcentrifuge tube and on the microfluidic device were 92–84%, 94–90%, 88.46–80.77%, and 95.92–91.84%, respectively. The limit of detection (LOD) was found to be the same as 1.5 × 102 CFU/mL and the limit of quantification (LOQ) values of the LAMP reactions were performed on the microcentrifuge tube and on the microfluidic device were 2.46 × 102–7.4 × 102 CFU/mL, respectively. Cohen’s kappa (κ) values of the LAMP reactions were performed on the microcentrifuge tube and on the microfluidic device were 0.620–0.705, respectively. In conclusion, our data showed that the LAMP method can be combined with microfluidic test device to detect S. pyogenes, this microfluidic device can be manufactured using 3D printers and results are close to gold standard methods. These devices can be combined with LAMP reactions to detect different pathogens where resources are limited and rapid results are required.

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“…Rapid diagnostic tests (RDTs) can reduce the time interval to use AST for guiding prescribing (36). RDTs using molecular techniques are now commercially available for the identification of various microbial etiological agents, including syphilis (37), invasive Streptococcus pyogenes (38), and helpful in differentiating bacterial from viral causes of respiratory diseases (39). This innovation has also led to several approved devices that can rapidly assess (<1-24hrs) the antimicrobial identification and sensitivity when there is infection (40,41).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial stewardship among Nigerian children: A pilot study of the knowledge, attitude, and practices of prescribers at two tertiary healthcare facilities in Bayelsa State

Orubu

Robert

Emuren

et al. 2021

Preprint

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Antimicrobial stewardship (AMS), the evidence-based use of antimicrobials, is an effective strategy in controlling antimicrobial resistance (AMR) in humans by reducing the irrational use of antimicrobials. Stewardship in children is less studied. This study assessed the knowledge, attitude, and practice of physicians prescribing antibiotics to children in Bayelsa State, Nigeria to identify gaps in AMS and possible solutions. Following ethical approval, a semi-structured questionnaire was distributed among 40 paediatricians and gynaecologists at the two public tertiary healthcare facilities in Bayelsa State – the Niger Delta University Teaching Hospital and the Federal Medical Centre – for self-completion. Responses were expressed as percentages and analyzed using Bloom’s cutoffs. The Capability, Opportunity, Motivation, and Behaviour (COM-B) model was employed to identify gaps for intervention in prescribing behavior with gaps in each component identified by aggregate scores <80%. Perceived approaches to improve prescribing among 14 selected options were assessed using 5-point Likert scales and options with scores >90% rated the most acceptable. Questionnaires were administered from August to September 2021. The response rate was 68% (27/40). Participants were paediatricians (81%, 22/27) and gynaecologists (19%, 5/27). Antimicrobial Susceptibility Testing (AST) was not performed before antibiotic selection nine times out of 10 (89%, 24/27). In a third (37%, 10/27) of cases, 2-3 antibiotics were prescribed. The top three antibiotics, in rank order, were: cefuroxime or amoxicillin 41% (11/27); ciprofloxacin or amoxicillin 30% (8/27), and azithromycin (33%, 9/27). Aggregate COM-B scores were: capability, 74%; opportunity, 78%; and motivation, 87%. The most acceptable (100%, 27/27) options to improving antibiotic prescribing were: availability of resistance data, availability of guidelines, readily accessible microbiological data, and easy access to infectious disease physicians. There are gaps in knowledge of AMR and opportunity for rational prescribing. There is need for antimicrobial resistance data to promote pediatric AMS at the surveyed healthcare facilities.

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Evaluation of a rapid diagnostic test for the detection of Streptococcus pyogenes in invasive infections

Cited by 5 publications

References 11 publications

<i>Candida glabrata</i> as an aetiological factor of the fulminant course of panophthalmitis

<i>Candida glabrata</i> as an aetiological factor of the fulminant course of panophthalmitis

Fabrication of a Microfluidic Test Device with a 3D Printer and Its Combination with the Loop Mediated Isothermal Amplification Method to Detect Streptococcus pyogenes

Antimicrobial stewardship among Nigerian children: A pilot study of the knowledge, attitude, and practices of prescribers at two tertiary healthcare facilities in Bayelsa State

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