Evaluation of a Rapid Immunochromatographic Assay for Identification of
            <i>Candida albicans</i>
            and
            <i>Candida dubliniensis</i>

Marot-Leblond, A.; Grimaud, Linda; David, Sandrine; Sullivan, Derek J.; Coleman, David C.; Pontón, José; Robert, Raymond

doi:10.1128/jcm.42.11.4956-4960.2004

Cited by 26 publications

(23 citation statements)

References 38 publications

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“…All had been identified by a number of techniques, including PCR based on the intron sequence of the ACT1 gene or immunofluorescence with a specific monoclonal antibody (20). Fresh clinical isolates of C. albicans and C. dubliniensis were also investigated, and their identities were confirmed by growth on modified Pal's agar (1,19).…”

Section: Methodsmentioning

confidence: 99%

“…More recently, it was reported that the two species could be distinguished by culture on Staib agar, Pal's agar, modified Pal's agar, and casein agar, on which only C. dubliniensis produces abundant chlamydospores and rough colonies (1,2,3,24,28). We also described an immunochromatographic assay differentiating between C. albicans and C. dubliniensis, but C. dubliniensis was identified only by default, owing to the specificity of the monoclonal antibodies (MAbs) (19). The aim of the present study was to produce a monoclonal antibody specific for C. dubliniensis cells and to investigate its potential use in a rapid latex agglutination test for identifying C. dubliniensis colonies.…”

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confidence: 99%

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Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies

Marot-Leblond¹,

Beucher²,

David³

et al. 2006

J Clin Microbiol

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Cell components of the dimorphic pathogenic fungus Candida dubliniensis were used to prepare monoclonal antibodies (MAbs). One MAb, designated 12F7-F2, was shown by indirect immunofluorescence to be specific for a surface antigen of Candida dubliniensis yeast cells. No reactivity was observed with other fungal genera or with other Candida species, including Candida albicans, that share many phenotypic features with C. dubliniensis. The use of different chemical and physical treatments for cell component extraction suggested that the specific epitope probably resides on a protein moiety absent from C. albicans. However, we failed to identify the target protein by Western blotting, owing to its sensitivity to heat and sodium dodecyl sulfate. MAb 12F7-F2 was further used to develop a commercial latex agglutination test to identify C. dubliniensis colonies (Bichrodubli Fumouze test; Fumouze Diagnostics). The test was validated on yeast strains previously identified by PCR and on fresh clinical isolates; these included 46 C. dubliniensis isolates, 45 C. albicans isolates, and other yeast species. The test had 100% sensitivity and specificity for C. dubliniensis isolated on Sabouraud dextrose, CHROMagar Candida, and CandiSelect media and 97.8% sensitivity for C. dubliniensis grown on Candida ID medium. The test is rapid (5 min) and easy to use and may be recommended for routine use in clinical microbiology laboratories and for epidemiological investigations.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies

Marot-Leblond¹,

Beucher²,

David³

et al. 2006

J Clin Microbiol

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“…The assay exploits the lateral-flow technology that has been used to date in tests for the detection of viruses, bacteria, and toxins (11,13,(28)(29)(30) and, most famously, for the home pregnancy tests first introduced by Unipath in 1988. While immunochromatographic assays have been developed for the identification of Candida species (19) and for the detection of fungi in soil (36,37), this is the first time, to the best of the author's knowledge, that an LFD has been developed for the detection of Aspergillus antigens in human serum.…”

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confidence: 99%

Development of an Immunochromatographic Lateral-Flow Device for Rapid Serodiagnosis of Invasive Aspergillosis

Thornton

2008

Clin Vaccine Immunol

205

186

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Aspergillus fumigatus is a cosmopolitan saprotrophic fungus that is second only toThe dramatic increase in the numbers of opportunistic infections of humans caused by Aspergillus species over the last decade is associated with a rise in the numbers of solid-organ transplants and the use of aggressive cancer therapies and other immunomodulating treatments (4, 15). The rate of mortality due to invasive aspergillosis (IA) has increased by 357% over the last 25 years; and IA has become one of the leading causes of death in immunocompromised patients, with mortality rates ranging from 60 to 90%

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“…Moreover, considerable experience is necessary for correct phenotypic identification of the steadily expanding range of pathogenic and opportunistic fungi. The discrepant result of one clinical specimen identified as C. dubliniensis by the Luminex assay and C. albicans by blood culture (Table 5) could be attributable to the major phenotypic (22) but only limited genetic similarities between these two species which have likely led to incorrect typing by routine fungus culture. Unequivocal identification of C. albicans and C. dubliniensis, however, may be relevant for the most appropriate antifungal treatment.…”

Section: Discussionmentioning

confidence: 99%

Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology

et al. 2009

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In immunocompromised patients suffering from invasive fungal infection, rapid identification of the fungal species is a prerequisite for selection of the most appropriate antifungal treatment. We present an assay permitting reliable identification of a wide range of clinically relevant fungal pathogens based on the highthroughput Luminex microbead hybridization technology. The internal transcribed spacer (ITS2) region, which is highly variable among genomes of individual fungal species, was used to generate oligonucleotide hybridization probes for specific identification. The spectrum of pathogenic fungi covered by the assay includes the most commonly occurring species of the genera Aspergillus and Candida and a number of important emerging fungi, such as Cryptococcus, Fusarium, Trichosporon, Mucor, Rhizopus, Penicillium, Absidia, and Acremonium. Up to three different probes are employed for the detection of each fungal species. The redundancy in the design of the assay should ensure unambiguous fungus identification even in the presence of mutations in individual target regions. The current set of hybridization oligonucleotides includes 75 speciesand genus-specific probes which had been carefully tested for specificity by repeated analysis of multiple reference strains. To provide adequate sensitivity for clinical application, the assay includes amplification of the ITS2 region by a seminested PCR approach prior to hybridization of the amplicons to the probe panel using the Luminex technology. A variety of fungal pathogens were successfully identified in clinical specimens that included peripheral blood, samples from biopsies of pulmonary infiltrations, and bronchotracheal secretions derived from patients with documented invasive fungal infections. Our observations demonstrate that the Luminex-based technology presented permits rapid and reliable identification of fungal species and may therefore be instrumental in routine clinical diagnostics.Although the vast majority of invasive fungal infections are still caused by Aspergillus or Candida species, changes in epidemiology have become evident over the last years (11,15,33,39). In view of the different drug resistance profiles of many clinically relevant fungal pathogens (34), the development of rapid methods for species-specific identification of clinically important fungi is desirable in order to permit selection of the most appropriate antifungal treatment. Traditional diagnostic approaches to the identification of fungal species are mainly based on phenotype analysis of fungal cultures. However, these approaches are time-consuming and show limited applicability for the detection of molds (29). Over the last years, a variety of molecular methods have been established for rapid and sensitive detection of fungal pathogens. Many of these assays are real-time quantitative PCR tests, mostly targeting the ribosomal multicopy gene (rDNA gene) (1, 3, 12-14, 16, 21, 30, 31, 35, 40). With these techniques, the fungal sequences of interest can be amplified by universa...

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Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis

Cited by 26 publications

References 38 publications

Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies

Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies

Development of an Immunochromatographic Lateral-Flow Device for Rapid Serodiagnosis of Invasive Aspergillosis

Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology

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