Evaluation of a real-time PCR assay for the differentiation of Bacillus cereus group species

Frentzel, Hendrik; Kelner-Burgos, Ylanna; Deneke, Carlus

doi:10.1016/j.foodcont.2020.107530

Cited by 4 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These species show phenotypic properties and a high level of similarity in their DNA, making biochemical identification quite difficult [ 22 ]. Different species had traditionally been differentiated by their phenotypic characteristics (e.g., shape, optimal growth temperature, resistance to acidity) but currently, other more powerful methods are being used such as digital DNA-DNA hybridization (dDDH) and/or average nucleotide identity (ANI) values [ 24 ], signature sequences (e.g., in the 16 S rRNA and cspA genes) or the presence of specific virulence factors (e.g., cytK-1 or anthrax toxin genes), and MALDI-TOF MS analysis [ 25 ] or real time PCR [ 26 ]. A recent study for Carroll [ 27 ] describes the first whole genome sequencing (WGS) characterization of isolates linked to an outbreak caused by members of the B. cereus group.…”

Section: Hazard Description and Growth In Ricementioning

confidence: 99%

Risk of Bacillus cereus in Relation to Rice and Derivatives

Rodrigo

Rosell

Martínez

2021

Foods

View full text Add to dashboard Cite

Rice is a very popular food throughout the world and the basis of the diet of the citizens of many countries. It is used as a raw material for the preparation of many complex dishes in which different ingredients are involved. Rice, as a consequence of their cultivation, harvesting, and handling, is often contaminated with spores of Bacillus cereus, a ubiquitous microorganism found mainly in the soil. B. cereus can multiply under temperature conditions as low as 4 °C in foods that contain rice and have been cooked or subjected to treatments that do not produce commercial sterility. B. cereus produces diarrhoeal or emetic foodborne toxin when the consumer eats food in which a sufficient number of cells have grown. These circumstances mean that every year many outbreaks of intoxication or intestinal problems related to this microorganism are reported. This work is a review from the perspective of risk assessment of the risk posed by B. cereus to the health of consumers and of some control measures that can be used to mitigate such a risk.

show abstract

Section: Hazard Description and Growth In Ricementioning

confidence: 99%

Risk of Bacillus cereus in Relation to Rice and Derivatives

Rodrigo

Rosell

Martínez

2021

Foods

View full text Add to dashboard Cite

show abstract

“…From the conventional plate culture method to enzyme-linked immunosorbent assay [4], immunochromatography [5], nucleic acid amplification technology [6] and molecular hybridization technology, and then to the application of various amplification strategies [7,8] and biosensing technologies, the detected species of pathogen detection technologies have been reduced from a single colony to a single cell, and even accuracy at the protein and nucleic acid level is available. The detection principles cover chemical reactions, immune reactions, nucleic acid hybridization and the superposition of multiple technologies.…”

Section: Introductionmentioning

confidence: 99%

Research progress of CRISPR/Cas systems in nucleic acid detection of infectious diseases

Dong

Zhou

2023

iLABMED

View full text Add to dashboard Cite

Infectious diseases are a serious threat to human health, and accurate, rapid and convenient early detection of pathogens is the first step of active treatment. Technologies that detect pathogens have advanced significantly because of the development of fundamental disciplines and the integration of multidisciplinary fields. Among these technologies, nucleic acid detection technology is preferred because of its rapid measurement, accuracy and high sensitivity. The CRISPR/Cas system, consisting of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated (Cas), is an adaptive immune system that specifically recognizes, binds and cleaves exogenous invasive nucleic acids. The CRISPR/Cas system is widely found in bacteria and archaea. Researchers have developed nucleic acid detection technologies with single-molecule sensitivity, single-base precision specificity, portability and low cost based on the specific cleavage and trans-cleavage activities of the CRISPR/Cas system. The next generation of in-vitro diagnostics is shifting to nucleic acid technology because this technology shows promise in a wide range of applications in resource-constrained environments.In this review, the development and mechanism of the CRISPR/Cas system are presented together with representative CRISPR/Cas applications in nucleic acid detection. Additionally, the review summarizes future perspectives and trends of the CRISPR/Cas system in nucleic acid detection.

show abstract