Evaluation of a real-time polymerase chain reaction assay for the detection of Dientamoeba fragilis

Calderaro, Adriana; Gorrini, Chiara; Montecchini, Sara; Peruzzi, Simona; Piccolo, Giovanna; Rossi, Sabina; Gargiulo, Franco; Manca, N.; Dettori, G.; Chezzi, Carlo

doi:10.1016/j.diagmicrobio.2010.02.016

Cited by 34 publications

(36 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Two D. fragilis-infected subjects (1%) were revealed by the culture method. It may be that the D. fragilis present were dead, or there may have been an over-growth of other protozoa in the stool samples that prevented D. fragilis replication [19]. The prevalence of D. fragilis was 5% with iron-hematoxylin staining.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Hamidi

Meamar

Akhlaghi

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Dientamoeba fragilis, an intestinal trichomonad, occurs in humans with and without gastrointestinal symptoms. Its presence was investigated in individuals referred to Milad Hospital, Tehran. Methodology: In a cross-sectional study, three time-separated fecal samples were collected from 200 participants from March through June 2011. Specimens were examined using traditional techniques for detecting D. fragilis and other gastrointestinal parasites: direct smear, culture, formalin-ether concentration, and iron-hematoxylin staining. The presence of D. fragilis was determined using PCR assays targeting 5.8S rRNA or small subunit ribosomal RNA. Results: Dientamoeba fragilis, Blastocystis sp., Giardia lamblia, Entamoeba coli, and Iodamoeba butschlii were detected by one or more traditional and molecular methods, with an overall prevalence of 56.5%. Dientamoeba was not detected by direct smear or formalin-ether concentration but was identified in 1% and 5% of cases by culture and iron-hematoxylin staining, respectively. PCR amplification of SSU rRNA and 5.8S rRNA genes diagnosed D. fragilis in 6% and 13.5%, respectively. Prevalence of D. fragilis was unrelated to participant gender, age, or gastrointestinal symptoms. Conclusions: This is the first report of molecular assays to screen for D. fragilis in Iran. The frequent finding of D. fragilis via fecal analysis indicated the need to include this parasite in routine stool examination in diagnostic laboratories. As the length of amplification target correlates to the sensitivity of PCR, this assay targeting the D. fragilis 5.8S rRNA gene seems optimal for parasite detection and is recommended in combination with conventional microscopy for diagnosing intestinal parasites.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The development of PCR has provided a highly sensitive and specific method for diagnosis of pathogenic protozoa. PCRbased assays using species-specific primers offer a convenient and reliable technique for the detection of D. fragilis [17,19].…”

Section: Introductionmentioning

confidence: 99%

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Hamidi

Meamar

Akhlaghi

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…However, realtime PCR surveys have been carried out in other developed nations, and such high incidences have not been reported elsewhere (49,156). It is also possible that this is a localized phenomenon, potentially attributable to local climate or the possible existence of a resilient, highly transmissible strain of D. fragilis that is endemic to the region.…”

Section: Clinical Aspects and Epidemiologymentioning

confidence: 99%

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

View full text Add to dashboard Cite

SUMMARYDientamoeba fragilisis a protozoan parasite of the human bowel, commonly reported throughout the world in association with gastrointestinal symptoms. Despite its initial discovery over 100 years ago, arguably, we know less about this peculiar organism than any other pathogenic or potentially pathogenic protozoan that infects humans. The details of its life cycle and mode of transmission are not completely known, and its potential as a human pathogen is debated within the scientific community. Recently, several major advances have been made with respect to this organism's life cycle and molecular biology. While many questions remain unanswered, these and other recent advances have given rise to some intriguing new leads, which will pave the way for future research. This review encompasses a large body of knowledge generated on various aspects ofD. fragilisover the last century, together with an update on the most recent developments. This includes an update on the latest diagnostic techniques and treatments, the clinical aspects of dientamoebiasis, the development of an animal model, the description of aD. fragiliscyst stage, and the sequencing of the firstD. fragilistranscriptome.

show abstract

“…Studies also highlight the lack of sensitivity demonstrated by microscopy, and thus, molecular methods are considered the diagnostic methods of choice for enteric protozoan parasites. However, until all potential human protozoan pathogens are included in the molecular panels, they will remain highly sensitive but will fail to detect all possible pathogens (28,(42)(43)(44)(45)(46)(47)(48)(49)(50)84). Although molecular procedures detect a high percentage of intestinal protozoa in pediatric patients with gastrointestinal symptoms, interpretation and determination of the clinical relevance of a positive PCR result in this population may remain somewhat difficult.…”

Section: Diagnosismentioning

confidence: 99%

Dientamoeba fragilis, One of the Neglected Intestinal Protozoa

Garcia¹

2016

J Clin Microbiol

View full text Add to dashboard Cite

Dientamoeba fragilis is a single-celled protozoan, closely related to the trichomonads. Reported worldwide as causing human gastrointestinal symptoms, D. fragilis is very common and is second only to Blastocystis spp. Dientamoebiasis equals or exceeds the incidence of giardiasis. This minireview includes diagnostic options, clinical relevance, therapy, an animal model, the confirmed cyst stage, and sequencing data. The development of a rodent model, fulfilling Koch's postulates, and the confirmation of a cyst stage have clarified transmission routes, including fecal-oral transmission. The prevalence of D. fragilis varies between 0% to over 82%; results depend on the geographic location, group studied, and diagnostic methods used.

show abstract

Evaluation of a real-time polymerase chain reaction assay for the detection of Dientamoeba fragilis

Cited by 34 publications

References 24 publications

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Dientamoeba fragilis diagnosis by fecal screening: relative effectiveness of traditional techniques and molecular methods

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Dientamoeba fragilis, One of the Neglected Intestinal Protozoa

Contact Info

Product

Resources

About