Evaluation of a recombinant MIC3 based latex agglutination test for the rapid serodiagnosis of Toxoplasma gondii infection in swines

Jiang, Tao; DaChun, Gong; Ma, Linna; Nie, Hongguang; Zhou, Yanqin; Yao, Bao-An; Zhao, Junlong

doi:10.1016/j.vetpar.2008.07.035

Cited by 37 publications

(19 citation statements)

References 12 publications

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“…Furthermore, the use of a combination of recombinant antigens may enhance the sensitivity of an antibodybased assay. Several previous studies have found that recombinant antigens improve the serological diagnosis of T. gondii infections (3,5,11,14,16,30). Moreover, recombinant antigens have the potential to be used in the creation of new tests capable of differentiating recently acquired infections from those acquired in the more distant past (1,15,20,27).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma gondii Infection

Dai¹,

Jiang²,

Wang³

et al. 2012

Clin. Vaccine Immunol.

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bDetection of Toxoplasma gondii infection with sensitive and specific methods is a key step in the prevention and treatment of toxoplasmosis. Among the available diagnostic tests, serology is commonly used. Although serological tests give satisfactory results, the production of reliable reagents remains laborious and expensive. There is therefore a real need to acquire specific and effective recombinant antigens for the serodiagnosis of T. gondii infection. In this study, a multiepitope peptide was designed and successfully expressed in Escherichia coli, and then IgG and IgM enzyme-linked immunosorbent assays (ELISAs) were developed and evaluated. Our results showed that the new multiepitope antigen is one of the most promising recombinant antigens which could be used in routine screening of human toxoplasmosis.

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Section: Discussionmentioning

confidence: 99%

Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma gondii Infection

Dai¹,

Jiang²,

Wang³

et al. 2012

Clin. Vaccine Immunol.

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“…Such testes are called indirect hemagglutination assays (IHA) and latex agglutination tests (LAT), respectively (Feng et al, 2016 ). The antigens used to sensitize agglutination particles were more or less the same as the ones used in indirect ELISAs (Chen et al, 1980 ; Yu, 2001 ; Jiang et al, 2008 ). In addition to agglutination tests and ELISAs, there are other serological methods reported, such as, indirect fluorescent assays (IFA) (Wang Y.…”

Section: Development Of Diagnostic Approachesmentioning

confidence: 99%

Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview

et al. 2017

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Toxoplasma gondii is a ubiquitous zoonotic pathogen belonging to apicomplexan parasites. Infection in humans and animals may cause abortion and other severe symptoms under certain circumstances, leading to great economical losses and public health problems. T. gondii was first discovered in China in 1955 and the corresponding work was published in 1957. Since then, a lot of work has been done on this parasite and the diseases it causes. This review summarizes the major progress made by Chinese scientists over the last 60 years, and gives our perspectives on what should be done in the near future. A wide variety of diagnostic approaches were designed, including the ones to detect T. gondii specific antibodies in host sera, and T. gondii specific antigens or DNA in tissue and environmental samples. Further work will be needed to translate some of the laboratory assays into reliable products for clinic uses. Epidemiological studies were extensively done in China and the sero-prevalence in humans increased over the years, but is still below the world average, likely due to the unique eating and cooking habits. Infection rates were shown to be fairly high in meat producing animals such as, pigs, sheep, and chickens, as well as in the definitive host cats. Numerous subunit vaccines in the form of recombinant proteins or DNA vaccines were developed, but none of them is satisfactory in the current form. Live attenuated parasites using genetically modified strains may be a better option for vaccine design. The strains isolated from China are dominated by the ToxoDB #9 genotype, but it likely contains multiple subtypes since different ToxoDB #9 strains exhibited phenotypic differences. Further studies are needed to understand the general biology, as well as the unique features of strains prevalent in China.

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“…It precisely assays IgG, IgM, IgA, and IgE antibodies. To support accurate serological interpretation and further assay optimization, it is important to establish proper positive controls [ 16 , 25 , 26 , 30 ]. Practically, naturally T. gondii -infected pigs are good references.…”

Section: Discussionmentioning

confidence: 99%

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets

et al. 2018

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To investigate the prevalence of Toxoplasma gondii in pork on the market in Korea, an in-house enzyme-linked immunosorbent assay for tissue fluid (CAU-tf-ELISA) was developed using a soluble extract of T. gondii RH strain tachyzoites. As the standard positive controls, the piglets were experimentally infected with T. gondii: Group A (1,000 cysts-containing bradyzoites), Group B (500 cysts-containing bradyzoites) and Group C (1.0×103 or 1.0×104 tachyzoites). The CAU-tf-ELISA demonstrated infection intensity-dependent positivity toward tissue fluids with average cut-off value 0.15: 100% for Group A, 93.8% for Group B and 40.6% for Group C. When tissue-specific cut-off values 0.066–0.199 were applied, CAU-tf-ELISA showed 96.7% sensitivity, 100% specificity, 100% positive and 90.0% negative predictive values. When compared with the same tissue fluids, performance of CAU-tf-ELISA was better than that of a commercial ELISA kit. Of the 583 Korea domestic pork samples tested, anti-T. gondii antibodies were detected from 9.1% of whole samples and 37.9% from skirt meat highest among pork parts. In the 386 imported frozen pork samples, 1.8% (skirt meat and shoulder blade) were positive for anti-T. gondii antibodies. In Korea, prevalence of anti-T. gondii antibodies in the pork on retail markets appeared high, suggesting that regulations on pig farming and facilities are necessary to supply safe pork on the tables.

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Evaluation of a recombinant MIC3 based latex agglutination test for the rapid serodiagnosis of Toxoplasma gondii infection in swines

Cited by 37 publications

References 12 publications

Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma gondii Infection

Evaluation of a Recombinant Multiepitope Peptide for Serodiagnosis of Toxoplasma gondii Infection

Sixty Years (1957–2017) of Research on Toxoplasmosis in China—An Overview

Tissue Fluid Enzyme-Linked Immunosorbant Assay for Piglets Experimentally Infected with Toxoplasma gondii and Survey on Local and Imported Pork in Korean Retail Meat Markets

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