1992
DOI: 10.1099/00221287-138-5-889
|View full text |Cite
|
Sign up to set email alerts
|

Evaluation of a ribosomal RNA gene probe for the identification of species and subspecies within the genus Staphylococcus

Abstract: To evaluate a 16s rRNA gene probe for the identification of staphylococcal species and subspecies, we have augmented previous studies involving 12 staphylococcal species by analysing the remaining 16 species currently classified in the genus Staphylococcus. HindIII-and EcoRI-restricted DNA of isolates from validly described species of Staphylococcus was probed with radiolabelled plasmid pBA2 containing 16s rDNA from Bacillus subtilis. The Dice coefficient was used to assess similarity between the 74 HindIII-an… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
5

Citation Types

2
62
1

Year Published

1993
1993
2013
2013

Publication Types

Select...
4
2
1

Relationship

0
7

Authors

Journals

citations
Cited by 83 publications
(65 citation statements)
references
References 36 publications
2
62
1
Order By: Relevance
“…Two established species, S. felis and S. simulans, which are indistinguishable by several species differentiation tables of phenotypic tests [17,20,[23][24][25] except for bacitracin resistance [21], were distinct in the phylogenetic tree with a relatively deep branch. In contrast, two subspecies of the species of S. aureus, aureus and anaerobius, were phenotypically different to each other [17], and this was supported by the results from ribotyping [7], however, their 16S rDNA sequences were nearly indistinguishable in this study. A nucleotide change was found at only one residue between type strains of the subspecies.…”
Section: Discussioncontrasting
confidence: 29%
See 2 more Smart Citations
“…Two established species, S. felis and S. simulans, which are indistinguishable by several species differentiation tables of phenotypic tests [17,20,[23][24][25] except for bacitracin resistance [21], were distinct in the phylogenetic tree with a relatively deep branch. In contrast, two subspecies of the species of S. aureus, aureus and anaerobius, were phenotypically different to each other [17], and this was supported by the results from ribotyping [7], however, their 16S rDNA sequences were nearly indistinguishable in this study. A nucleotide change was found at only one residue between type strains of the subspecies.…”
Section: Discussioncontrasting
confidence: 29%
“…In other words, there is only a limited number of stable discriminating characteristics to distinguish one taxon supported phylogenetically from another by phenotypic tests [17]. Although our cluster groups correlated well with the species groups and other genealogical taxonomies such as ribotypes [6][7][8], several disagreements were found. Two established species, S. felis and S. simulans, which are indistinguishable by several species differentiation tables of phenotypic tests [17,20,[23][24][25] except for bacitracin resistance [21], were distinct in the phylogenetic tree with a relatively deep branch.…”
Section: Discussionmentioning
confidence: 61%
See 1 more Smart Citation
“…SEB + strains differed from the SEB -control strains in that they were more homogenous when examined by ribotyping (38 SEB + strains in 8 ribotypes versus 30 SEB-strains in 24 ribotypes), zymotyping (mean genetic heterogeneity diversity was 0 220 for SEB +, 0 295 for SEB-), and pulsed-field electrophoresis (39 SEB + in 25 types versus 30 SEB-in 28 types).…”
Section: Discussionmentioning
confidence: 97%
“…This property is undoubtedly due to the fact that the method is applicable to the taxonomy of staphylococci [23,24]. It nonetheless discriminated between methicillin-susceptible and -resistant strains.…”
Section: Discussionmentioning
confidence: 99%