Evaluation of a ristocetin bonded stationary phase for subcritical fluid chromatography of enantiomers

“…The effects of temperature and alcohol modifier were also reported. A ristocetin CSP was evaluated for chiral separations in subcritical fluid chromatography, with solutes of differing structures and pK a values tested (98). The effects of modifiers, additives, temperature, and flow rate on the enantioseparations were presented.…”

Section: Supercritical Fluid Chromatography and Related Techniquesmentioning

confidence: 99%

Chiral Separations

2004

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Gas Chromatography (GC). The separation of enantiomers by GC on CSPs was extensively reviewed by Schurig (12), with discussion of the method development, applications, and ancillary techniques of chiral separations using GC. CSPs with amino acid derivatives, terpene-derived metal coordination compounds, and modified cyclodextrins were included. Schurig also comprehensively reviewed the practice and theory of enantioselective complexation GC on optically active metal(II) bis[3-perfluoroacyl)-(1R)-camphorate] selectors (13). Applications extend to chiral analysis in asymmetric synthesis, enzymic reactions, pheromone, and flavor chemistry. The elucidation of thermodynamic parameters of enantioselectivity and the investigation of the enantiomerization of configurationally labile enantiomers was also discussed.Thin-Layer Chromatography (TLC). The development of stationary phases for TLC in the last 10 years was compiled by Gocan ( 14), and included chiral separation and recent advances in chiral stationary phases. The CSPs discussed included nonpolar bonded stationary phases impregnated with transitional metal ions, cellulose, modified cellulose, chitin, chitosan, and their derivatives. Cyclodextrin and macrocyclic antibiotics were reported to have very good results for enantioseparation by TLC, with molecular imprinting polymers also finding use as CSPs in TLC.Capillary Electrochromatography. The applications of CEC were reviewed by Remcho et al. (15). Chiral separations with cyclodextrins and their derivatives, biomolecules, molecularly imprinted polymers, "brush"-type phases, ion exchangers, antibiotics, polysaccharide derivatives, and other CSPs were included in this review. A summary of recent progress in open-tubular CEC for chiral and achiral separations included stationary-phase preparation ( 16), with the major developments, potential applications, technical difficulties, and advantages of each wall coating discussed.Multiple Technique Reviews. The use of cyclodextrins in chiral chromatography was compiled by Juvancz and Szejtli (17). The role of cyclodextrins in methods using capillary columns such as GC, supercritical fluid chromatography (SFC), and CE was detailed, as well as their use in other forms of chromatography such as HPLC and TLC. The mechanism of chiral recognition using cyclodextrins was also discussed. Williams and Wainer (18) reviewed the role of chiral chromatography in therapeutic drug monitoring and in clinical and forensic toxicology. Enantioselective GC and HPLC were used as tools to unravel complex phenomena associated with drug transport and metabolism.

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“…For comparison: on the Chiralpak AD column the additives had little effect on retention, but they did foster significant improvements in peak shape and resolution of tested chiral compounds. Some tentative experiments were also performed using ristocetin‐A‐based (Chirobiotic R) column 89 . Figure 5 shows an example of fast baseline enantioseparation of efavirenz enantiomers using 250 mm long Chirobiotic R column.…”

Section: Theoretical Studies Of Macrocyclic Glycopeptides In Sfcmentioning

confidence: 99%

Macrocyclic glycopeptide‐based chiral selectors for enantioseparation in sub/supercritical fluid chromatography

Folprechtová

Kalíková

2020

Analytical Science Advances

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Increasing number of reported works dealing with macrocyclic glycopeptide‐based columns in sub/supercritical fluid chromatography (SFC) points to the growing interest in this area. With the development and production of sub 2 µm fully porous particles and superficially porous particles with bonded macrocyclic glycopeptides, significant improvements have been made in ultrafast high efficiency chiral SFC. This review article gives an overview of macrocyclic glycopeptide‐based chiral selectors that were used in theoretical studies and/or applications in SFC. The review covers the period from 1997 when macrocyclic glycopeptides were first used in SFC till the end of July 2020 according to Web of Science. This work can also be helpful to analysts searching for an appropriate method for the separation/determination of enantiomers of their interest.

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Evaluation of a ristocetin bonded stationary phase for subcritical fluid chromatography of enantiomers

Cited by 19 publications

References 14 publications

Supercritical fluid chromatography for the enantioseparation of pharmaceuticals

Supercritical fluid chromatography for the enantioseparation of pharmaceuticals

Chiral Separations

Macrocyclic glycopeptide‐based chiral selectors for enantioseparation in sub/supercritical fluid chromatography

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