Evaluation of a rosette inhibition test for pregnancy diagnosis in pigs

Grewal, A. S.; Wallace, Andrew L.; Pan, Ye; Rigby, N.W.; Donnelly, J.B.; Eagleson, G. K.; Nancarrow, C. D.

doi:10.1016/0165-0378(85)90067-1

Cited by 5 publications

(2 citation statements)

References 18 publications

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“…A primary reguirement for the rosette inhibition assay in order to detect the presence of EPF is an effective antilymphocyte serum (ALS) which gives the inhibition of rosette formation (Morton et al, 1982 b;Grewal et al, 1985). In our experience, the polyclonal antibodies (with high titre) to human lymphocytes worked well.…”

Section: Discussionmentioning

confidence: 78%

Early Pregnancy Factor (EPF) as a Marker for the Diagnosis of Subclinical Embryonic Loss

Shahani

Moniz²,

Chitlange³

et al. 2009

Exp Clin Endocrinol Diabetes

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The validation of EPF as a possible correlate of early fertilization has made it possible to study and detect fertilization of the ovum in normal fertile women (during the luteal phase) and also in women with infertility, where the fertilization of the ovum may not be affected but there may be impairment in early embryonic development which results in early embryo loss or subclinical embryo loss. Our results have suggested that using EPF as a marker, we could detect subclinical embryonic loss in 57.8% of the infertile women where more than one menstrual cycle was studied and the blood was collected 4-7 days after ovulation. After the missed period, 80% of the patients who were negative for EPF but positive for hCG had spontaneous abortions. It would be interesting to study how EPF behaves as a marker, to detect subclinical embryonic loss in diverse pathological situations such as recurrent abortions, parental age and translocation carrier parents.

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Section: Discussionmentioning

confidence: 78%

Early Pregnancy Factor (EPF) as a Marker for the Diagnosis of Subclinical Embryonic Loss

Shahani

Moniz²,

Chitlange³

et al. 2009

Exp Clin Endocrinol Diabetes

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“…EPF is reported to be present in the pregnant sera of most mammalian species including humans [56, 57], mice [51], sheep [58, 59], cows [60–62], pigs [63], mares [64], and some wild animals [65]. In buffalo pregnancy, Chander [66] demonstrated decreased E-rosette formation but failed to demonstrate the presence of a rosette inhibiting factor (RIF, which probably would have been EPF) in the serum.…”

Section: Pregnancy Detection Methodsmentioning

confidence: 99%

Early Pregnancy Diagnosis in Bovines: Current Status and Future Directions

Balhara

Gupta

Singh

et al. 2013

The Scientific World Journal

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An early and accurate diagnosis of reproductive dysfunctions or aberrations is crucial to better reproductive management in livestock. High reproductive efficiency is a prerequisite for high life-time production in dairy animals. Early pregnancy diagnosis is key to shorten the calving interval through early identification of open animals and their timely treatment and rebreeding so as to maintain a postpartum barren interval close to 60 days. A buffalo, the most important dairy animal in the Indian subcontinent, is known for problems related to high calving interval, late puberty, and high incidence of anestrus. Lack of reliable cow-side early pregnancy diagnosis methods further aggravates the situation. Several methods of pregnancy diagnosis are being practiced in bovine species, yet none qualifies as the ideal pregnancy diagnosis method due to the inherent limitations of sensitivity, accuracy, specificity, speed, and ease of performing the test. The advancement of molecular techniques like proteomics and their applications in animal research has given a new hope to look for pregnancy biomarker molecules in these animals. This review attempts to examine common pregnancy diagnosis methods available for dairy animals, while assessing the usefulness of the modern technologies in detecting novel pregnancy markers and designing future strategies for research in this area.

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Pregnancy and Its Diagnosis

Taverne¹,

Noakes²

2019

Veterinary Reproduction and Obstetrics

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Evaluation of a rosette inhibition test for pregnancy diagnosis in pigs

Cited by 5 publications

References 18 publications

Early Pregnancy Factor (EPF) as a Marker for the Diagnosis of Subclinical Embryonic Loss

Early Pregnancy Factor (EPF) as a Marker for the Diagnosis of Subclinical Embryonic Loss

Early Pregnancy Diagnosis in Bovines: Current Status and Future Directions

Pregnancy and Its Diagnosis

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