Evaluation of a self-fitting, shape memory polymer scaffold in a rabbit calvarial defect model

Pfau, Michaela R.; Beltran, Felipe O.; Woodard, Lindsay N.; Dobson, Lauren K.; Gasson, Shelby; Robbins, Andrew B.; Lawson, Zachary T.; Saunders, W. B.; Moreno, Michael R.; Grunlan, Melissa A.

doi:10.1016/j.actbio.2021.09.041

Cited by 27 publications

(18 citation statements)

References 42 publications

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“…CT examination at the last follow-up shows incomplete healing in both untreated and scaffold-treated defects. However, relevant differences are observed in the assessment of the area covered by mineralised tissue, in agreement with similar studies [ 18 , 19 ].…”

Section: Discussionsupporting

confidence: 91%

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Improved Bone Regeneration Using Biodegradable Polybutylene Succinate Artificial Scaffold in a Rabbit Model

Vigni

Cassata

Caldarella

et al. 2022

JFB

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The treatment of extensive bone loss represents a great challenge for orthopaedic and reconstructive surgery. Most of the time, those treatments consist of multiple-stage surgeries over a prolonged period, pose significant infectious risks and carry the possibility of rejection. In this study, we investigated if the use of a polybutylene succinate (PBS) micro-fibrillar scaffold may improve bone regeneration in these procedures. In an in vivo rabbit model, the healing of two calvarial bone defects was studied. One defect was left to heal spontaneously while the other was treated with a PBS scaffold. Computed tomography (CT) scans, histological and immunohistochemical analyses were performed at 4, 12 and 24 weeks. CT examination showed a significantly larger area of mineralised tissue in the treated defect. Histological examination confirmed a greater presence of active osteoblasts and mineralised tissue in the scaffold-treated defect, with no evidence of inflammatory infiltrates around it. Immunohistochemical analysis was positive for CD56 at the transition point between healthy bone and the fracture zone. This study demonstrates that the use of a PBS microfibrillar scaffold in critical bone defects on a rabbit model is a potentially effective technique to improve bone regeneration.

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Section: Discussionsupporting

confidence: 91%

“…At the end of the procedure, each subject had two bone defects. One defect was treated by applying the scaffold while the other was left to heal spontaneously [ 18 ]. No suturing of the periosteum is performed.…”

Section: Methodsmentioning

confidence: 99%

Improved Bone Regeneration Using Biodegradable Polybutylene Succinate Artificial Scaffold in a Rabbit Model

Vigni

Cassata

Caldarella

et al. 2022

JFB

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“…Both star -PCL ( T m = ∼45 °C, compressive modulus = ∼3.57 MPa) and linear -PCL ( T m = ∼55 °C; compressive modulus = ∼9.65 MPa) scaffolds have been produced from the corresponding acrylated macromers, yielding scaffolds with the ability to undergo press-fitting and shape recovery within a defect after exposure to temperatures above their melt transition ( T m ) . These bone-regenerative PCL scaffolds also possess biodegradability and pore interconnectivity (pore diameter ∼220 μm) to facilitate osteoinduction but do not possess any adhesive properties, which limits their usability for the treatment of confined bone defects. , Scanning electron microscopy (SEM) images show that the 1-S2-FT01 resin penetrates 1 mm into the PCL scaffolds, causing physical interlocking of the PCL and resin and contributing to a strong adhesive bond (Figure A). PCL scaffolds adhered to the 1-S2-FT01 resin with maximum strengths in the MPa range (Figure B).…”

Section: Resultsmentioning

confidence: 99%

High-Throughput Screening of Thiol–ene Click Chemistries for Bone Adhesive Polymers

Ganabady,

Contessi Negrini,

Scherba

et al. 2023

ACS Appl. Mater. Interfaces

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Metal surgical pins and screws are employed in millions of orthopedic surgical procedures every year worldwide, but their usability is limited in the case of complex, comminuted fractures or in surgeries on smaller bones. Therefore, replacing such implants with a bone adhesive material has long been considered an attractive option. However, synthesizing a biocompatible bone adhesive with a high bond strength that is simple to apply presents many challenges. To rapidly identify candidate polymers for a biocompatible bone adhesive, we employed a high-throughput screening strategy to assess human mesenchymal stromal cell (hMSC) adhesion toward a library of polymers synthesized via thiol−ene click chemistry. We chose thiol−ene click chemistry because multifunctional monomers can be rapidly cured via ultraviolet (UV) light while minimizing residual monomer, and it provides a scalable manufacturing process for candidate polymers identified from a high-throughput screen. This screening methodology identified a copolymer (1-S2-FT01) composed of the monomers 1,3,5-triallyl-1,3,5-triazine-2,4,6(1H,3H,5H)-trione (TATATO) and pentaerythritol tetrakis (3-mercaptopropionate) (PETMP), which supported highest hMSC adhesion across a library of 90 polymers. The identified copolymer (1-S2-FT01) exhibited favorable compressive and tensile properties compared to existing commercial bone adhesives and adhered to bone with adhesion strengths similar to commercially available bone glues such as Histoacryl. Furthermore, this cytocompatible polymer supported osteogenic differentiation of hMSCs and could adhere 3D porous polymer scaffolds to the bone tissue, making this polymer an ideal candidate as an alternative bone adhesive with broad utility in orthopedic surgery.

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“…As already mentioned, the use of biomaterials for critical and load-bearing bone defects would indeed require an adequate mechanical strength able to mimic native tissue properties and sustain the newly forming bone without affecting its mechanical and functional performance, which is a difficult goal to achieve with polymeric materials. For this reason, despite the great advantage given by polymer biodegradability, the use of polymer-based porous scaffolds is often limited to the repair of noncritical bone defects, as in the craniomaxillofacial region [77][78][79]. The interaction of bone substitutes with biological fluids is another relevant parameter to be considered.…”

Section: Discussionmentioning

confidence: 99%

Advantages of agarose on alginate for the preparation of polysaccharide/hydroxyapatite porous bone scaffolds compatible with a proline-rich antimicrobial peptide

Mardirossian,

Gruppuso,

Guagnini

et al. 2023

Biomed. Mater.

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The optimized proline-rich antimicrobial peptide B7-005 was loaded on bone scaffolds based on polysaccharides and hydroxyapatite. Alginate was firstly chosen in order to exploit its negative charges, which allowed an efficient B7-005 loading but hindered its release, due to the strong interactions with the positive charged peptide. Hence, alginate was substituted with agarose which allowed to prepare scaffolds with similar structure, porosity, and mechanical performance than the ones prepared with alginate and hydroxyapatite. Moreover, agarose scaffolds could release B7-005 within the first 24 hours of immersion in aqueous environment. The peptide did not impaired MG-63 cell adhesion and proliferation in the scaffold, and a positive cell proliferation trend was observed up to 2 weeks. The released B7-005 was effective against the pathogens E. coli, K. pneumoniae, and A. baumannii, but not against S. aureus and P. aeruginosa, thus requiring further tuning of the system to improve its antimicrobial activity.

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Evaluation of a self-fitting, shape memory polymer scaffold in a rabbit calvarial defect model

Cited by 27 publications

References 42 publications

Improved Bone Regeneration Using Biodegradable Polybutylene Succinate Artificial Scaffold in a Rabbit Model

Improved Bone Regeneration Using Biodegradable Polybutylene Succinate Artificial Scaffold in a Rabbit Model

High-Throughput Screening of Thiol–ene Click Chemistries for Bone Adhesive Polymers

Advantages of agarose on alginate for the preparation of polysaccharide/hydroxyapatite porous bone scaffolds compatible with a proline-rich antimicrobial peptide

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