Evaluation of a simple and low cost potentiometric biosensor for pharmaceutical and in vivo adrenaline determination

Mataveli, Lidiane Raquel Verola; Antunes, Nilson; Brigagão, Maı́sa Ribeiro Pereira Lima; Magalhães, Cristiana Schmidt de; Wisniewski, Célio; Luccas, Pedro Orival

doi:10.1016/j.bios.2010.06.021

Cited by 15 publications

(11 citation statements)

References 38 publications

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“…The LOD value obtained is between the one reported by Tembe et al (1 × 10 -5 M) 27 and that obtained by Mataveli et al (8.0 × 10 -7 M). 28 Thus, the biosensor is able to meet the requirements for practical application.…”

mentioning

confidence: 99%

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Apetrei¹,

Apetrei²

2013

IJN

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A biosensor comprising tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode has been developed. The sensitive element, ie, tyrosinase, was immobilized using a drop-and-dry method followed by cross-linking. Tyrosinase maintained high bioactivity on this nanomaterial, catalyzing the oxidation of epinephrine to epinephrine-quinone, which was electrochemically reduced (−0.07 V versus Ag/AgCl) on the biosensor surface. Under optimum conditions, the biosensor showed a linear response in the range of 10–110 μM. The limit of detection was calculated to be 2.54 μM with a correlation coefficient of 0.977. The repeatability, expressed as the relative standard deviation for five consecutive determinations of 10 −5 M epinephrine solution was 3.4%. A good correlation was obtained between results obtained by the biosensor and those obtained by ultraviolet spectrophotometric methods.

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mentioning

confidence: 99%

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Apetrei¹,

Apetrei²

2013

IJN

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“…25 g of banana (Musa sp.) were used for each extract, added to 100 mL of dihydrogenphosphate buffer 0.1 mol·L -1 (pH = 7.00) and 2.5 g of polyvinylpyrrolidone (PVP) [12].…”

Section: Procedures For Extraction Of the Ppo Enzymesmentioning

confidence: 99%

“…In previous work, good results, in terms of detection limits, linear range and sensibility, were achieved with a simple potentiometric transducer [12]. This study's objective was to investigate PPO extraction procedures from banana tissue to improve biosensor performance.…”

Section: Introductionmentioning

confidence: 99%

“…tyrosine, phenol, p-cresol) and diphenols (e.g. catechol, L-dopa, dopamine, adrenaline) [9][10][11] leading to o-quinone, which then can polymerize and form melanin [12]. The PPO is widely distributed in nature, and found in various fruit and vegetable tissues [6,13].…”

Section: Introductionmentioning

confidence: 99%

“…Amperometry, spectrophotometry and potentiometry have been employed as transducers [12]. The potentiometric transducer is very common in analytical laboratories, due to its use in a simple pH-meter.…”

Section: Introductionmentioning

confidence: 99%

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Enzyme from Banana (<i>Musa</i> sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction

Silva¹,

Wisniewski²,

Luccas³

et al. 2013

AJAC

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For building a biosensor, at least two enzyme sources can be employed. The pure enzyme has features with better selectivity and low stability. Crude extract presents better stability and wrong selectivity. Thus, one intermediate condition can be feasible joining both benefits of crude extract and pure enzymes. For that result, several procedures of extraction and semi purification of polyphenol oxidase (PPO) enzyme from banana (Musa sp.) were studied. The results showed that cleaned enzymatic extracts presented higher specific activities than crude extracts (2.8 up to 5.3 fold), despite the total protein concentration diminishing from 27% up to 72%, indicating that polyphenol oxidase (PPO) enzyme was preserved in them. The biosensors with 125 AU mL -1 containing cleaned enzymatic extracts performed better by grinding or grinding plus sonication for 30 s. They were linear over the ranges of 5.9 × 10 -6 mol·L -1 to 1.4 × 10 -3 mol·L -1 and 7.9 × 10 -8 mol·L -1 to 4.0 × 10 -3 mol·L -1 , respectively. The limit of Detection (LOD) was 5.9 × 10 -6 mol·L -1 and 7.9 × 10 -8 mol·L -1 , respectively. The LOD obtained is adequate to adrenaline determination on blood and medicinal samples, and were applied in medicinal samples with satisfactory results.

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Measurement of Environmental Pollution: Types and Techniques

Kumar

Pathak

Chauhan

et al. 2017

Advances in Environmental Biotechnology

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Evaluation of a simple and low cost potentiometric biosensor for pharmaceutical and in vivo adrenaline determination

Cited by 15 publications

References 38 publications

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Enzyme from Banana (<i>Musa</i> sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction

Measurement of Environmental Pollution: Types and Techniques

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Evaluation of a simple and low cost potentiometric biosensor for pharmaceutical and in vivo adrenaline determination

Cited by 15 publications

References 38 publications

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Biosensor based on tyrosinase immobilized on a single-walled carbon nanotube-modified glassy carbon electrode for detection of epinephrine

Enzyme from Banana (&lt;i&gt;Musa&lt;/i&gt; sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction

Measurement of Environmental Pollution: Types and Techniques

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Product

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Enzyme from Banana (<i>Musa</i> sp.) Extraction Procedures for Sensitive Adrenaline Biosensor Construction