Evaluation of a tertiary amine labeling protocol for peptides and proteins using Ru(bpy)33+-based chemiluminescence detection

Waguespack, Brent L; Lillquist, Amy; Townley, Jonathan C; Bobbitt, Donald R.

doi:10.1016/s0003-2670(01)01081-9

Cited by 21 publications

(11 citation statements)

References 11 publications

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“…The use of electrogenerated tris(2,2 0 -bipyridine) ruthenium(II) (Ru bpy ð Þ 2þ 3 ) as a chemiluminescent reagent in ECL detection was first reported by Tokel and Bard [34], and investigated as a sensitive detection method in chemical analysis later. Ru bpy ð Þ 2þ 3 -based ECL detection has been used to detect a variety of chemical analytes such as chloroquine phosphate [35], rifampicin [36], peptide [37][38][39], amino acids [40], lidocaine [41], aspartate aminotransferase, and alanine aminotransferase [42]. Some reviews have demonstrated the proposed mechanism for the Ru bpy ð Þ 2þ 3 -based ECL [28,32,33], and the general mechanism of this system is the oxidation-reduction mode as follows: Ru bpy ð Þ * decays to the ground state and emits luminescence (iv).…”

Section: Introductionmentioning

confidence: 99%

Determination of ascorbic acid in individual rat hepatocyte cells based on capillary electrophoresis with electrochemiluminescence detection

Sun

Niu

et al. 2008

Electrophoresis

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A novel method to detect ascorbic acid (AA) in individual rat hepatocyte cells was developed by combining CE with electrochemiluminescence (ECL) based on tris(2,2'-bipyridine) ruthenium(II) (Ru(bpy)(3)2+). A single cell, followed by 0.1% SDS as cell lysis solution, was injected into the inlet of the separation capillary by electromigration. After optimizing the analytical conditions, the RSDs of migration time and peak height were 0.38% and 2.6% for 1.0x10(-5) M AA (n=10), respectively. The linear range of AA was from 1.0x10(-8) to 5.0x10(-5) M with a correlation coefficient of 0.9979 and the LOD (S/N=3) was estimated to be 1.0x10(-8) M. This method has been successfully applied to determine AA in single rat hepatocytes and the amount of AA in seven rat hepatocytes ranged from 16 to 62 fmol. The above results demonstrated that CE coupled with ECL is convenient, sensitive, and will become an attractive alternative method for single-cell analysis.

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Section: Introductionmentioning

confidence: 99%

Determination of ascorbic acid in individual rat hepatocyte cells based on capillary electrophoresis with electrochemiluminescence detection

Sun

Niu

et al. 2008

Electrophoresis

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“…The physiochemical properties of these complexes have been studied in detail [133,134]. There have been some reports dealing with the detection of amino acids and amines using tris(2,2 -bipyridyl) ruthenium(III) Ru(bpy) 3 3+ -based chemiluminescence [135][136][137]. Brune and Bobbitt [135] proposed a new technique for the detection of underivatized amino acids and amines using Ru(bpy) 3 3+ as a chemiluminescent reagent.…”

Section: Chemiluminescence Probesmentioning

confidence: 99%

“…Their detection limits are reported in the picomole to femole range (1-10 nmol/l) [136]. Recently, Waguespack et al [137] evaluated the properties of four ruthenium complexes, viz. bis(bipyridyl) bipyrimidine ruthenium (Ru(bpy) 2 (bpym) 3+ ), bis(bipyridyl)biimidazole ruthenium (Ru(bpy) 2 (biim) 2+ ), tris(4,7-diphenyl-1,10-phenanthroline) ruthenium (Ru(phen) 3 2+ ) and tris(bipyridyl)ruthenium (Ru(bpy) 3 3+ ) and their efficiency as probes for chemiluminescence detection.…”

Section: Chemiluminescence Probesmentioning

confidence: 99%

Advances in the study of luminescence probes for proteins

Sun

Yang

et al. 2004

Journal of Chromatography B

103

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“…Therefore, to enhance detection, most analytical procedures rely upon derivatization where an appropriate probe is attached to the protein of interest. Such probes can be found that exhibit CL properties [12]. The present CL detection is based on the reaction of 3-aminophthalic hydrazide (luminol) and hydrogen peroxide in the presence of a probe, offering excellent analytical sensitivity.…”

Section: Introductionmentioning

confidence: 99%

A novel probe for chemiluminescent image detection of proteins in two‐dimensional gel electrophoresis

Liu¹,

Ouyang²,

Baeyens³

et al. 2008

Electrophoresis

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Carrier ampholytes were found to enhance the chemiluminescence (CL) emission from the 3-aminophthalic hydrazide (luminol)-hydrogen peroxide system. They can be used as a chemiluminescent probe for rapid detection of major proteins in gels. This probe attracted much interest due to its ability to attach proteins, and to the possibility to combine it with separation techniques generating the CL emission directly. Increased signal intensity was achieved employing optimized concentrations of the carrier ampholyte enhancer. The binding of carrier ampholyte to proteins was found to occur at the pI of the proteins. Proteins from different regions of the gels were identified by their matrix-assisted TOF mass spectra and by appropriate database search, the results illustrating the possibility of major protein detection in human serum. Direct CL image detection with the carrier ampholyte probe can be applied for the detection of characteristic proteins in patients, i.e., proteins which cannot be detected without the probe.

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Evaluation of a tertiary amine labeling protocol for peptides and proteins using Ru(bpy)33+-based chemiluminescence detection

Cited by 21 publications

References 11 publications

Determination of ascorbic acid in individual rat hepatocyte cells based on capillary electrophoresis with electrochemiluminescence detection

Determination of ascorbic acid in individual rat hepatocyte cells based on capillary electrophoresis with electrochemiluminescence detection

Advances in the study of luminescence probes for proteins

A novel probe for chemiluminescent image detection of proteins in two‐dimensional gel electrophoresis

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