Evaluation of a thermo-tolerant acidophilic alga, Galdieria sulphuraria, for nutrient removal from urban wastewaters

Selvaratnam, Thinesh; Pegallapati, Ambica; Montelya, Felly; Rodriguez, G.; Nirmalakhandan, Nagamany; Voorhies, Wayne Van; Lammers, Peter J.

doi:10.1016/j.biortech.2014.01.075

Cited by 103 publications

(51 citation statements)

References 15 publications

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“…The G. sulfuraria strain examined here was isolated from hot springs in Yellowstone Park (51) but has properties that are very different from those of YNP 1A: it is larger, has a different intracellular organization (compare Fig. S9 and S5B in the supplemental material), and can grow on a wide range of carbon sources (51)(52)(53), whereas YNP 1A and its sister C. merolae are obligate phototrophs.…”

Section: Resultsmentioning

confidence: 99%

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens

et al. 2015

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Eisosomes are among the few remaining eukaryotic cellular differentations that lack a defined function(s). These trough-shaped invaginations of the plasma membrane have largely been studied in Saccharomyces cerevisiae, in which their associated proteins, including two BAR domain proteins, have been identified, and homologues have been found throughout the fungal radiation. Using quick-freeze deep-etch electron microscopy to generate high-resolution replicas of membrane fracture faces without the use of chemical fixation, we report that eisosomes are also present in a subset of red and green microalgae as well as in the cysts of the ciliate Euplotes. Eisosome assembly is closely correlated with both the presence and the nature of cell walls. Microalgal eisosomes vary extensively in topology and internal organization. Unlike fungi, their convex fracture faces can carry lineagespecific arrays of intramembranous particles, and their concave fracture faces usually display fine striations, also seen in fungi, that are pitched at lineage-specific angles and, in some cases, adopt a broad-banded patterning. The conserved genes that encode fungal eisosome-associated proteins are not found in sequenced algal genomes, but we identified genes encoding two algal lineage-specific families of predicted BAR domain proteins, called Green-BAR and Red-BAR, that are candidate eisosome organizers. We propose a model for eisosome formation wherein (i) positively charged recognition patches first establish contact with target membrane regions and (ii) a (partial) unwinding of the coiled-coil conformation of the BAR domains then allows interactions between the hydrophobic faces of their amphipathic helices and the lipid phase of the inner membrane leaflet, generating the striated patterns.

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Section: Resultsmentioning

confidence: 99%

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens

et al. 2015

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“…But only limited types of wastewaters can be treated because wastewaters are generally acidic and most algal species grow with difficulty at low pH, and absorption rates that can be achieved by bioaccumulation decrease substantially [46][47][48][49]. The acidophilic alga G. sulphuraria is the only alga that has commercial potential for remediation of these wastewaters [50,51]. Nutrient removal from municipal wastewater by the alga G. sulphurea was found to be very efficient for ammoniacal-nitrogen (88.3%) and phosphate (95.5%) in large scale outdoor bioreactors [51].…”

Section: Wastewatersmentioning

confidence: 99%

The Red MicroalgaGaldieriaas a Promising Organism for Applications in Biotechnology

Čížková¹,

Vítová²,

Zachleder³

2020

Microalgae - From Physiology to Application

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The genus Galdieria refers to red algae and includes microscopic inhabitants of highly acidic (pH 1-2), often volcanic habitats. They are thermophilic or thermo-tolerant organisms, some of them surviving temperatures up to 56°C. As other extremophilic microorganisms, they exhibit unique features derived from their modified metabolisms. In this chapter, we will review the special abilities of Galdieria species such as metabolic flexibility to grow photoautotrophically, heterotrophically or mixotrophically, ability to utilize a whole range of unusual carbon sources, capability of surviving extreme environments or their extremely high resistance to metals. We will discuss the potential of Galdieria for applications in biotechnology, for example, phycocyanin production, nutrient removal from urban wastewaters, bio-mining, treatment of acidic mine drainage, selective metal precipitation, bioremediation of acidic metal-contaminated areas or recovery of critical and scarce metals from secondary sources.

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“…Additionally, perhaps as acidic environments increase the solubility of most cations, G. sulphuraria bioaccumulates numerous rare-earth metals and several precious metals (Minoda, Sawada et al 2015, Ju, Igarashi et al 2016. In this versatile growth state, interest in this organism has been seen in the industrial biotechnology sector, both for remediation of wastes and in the production of high value chemicals (Schmidt, Wiebe et al 2005, Selvaratnam, Pegallapati et al 2014, Henkanatte-Gedera, Selvaratnam et al 2015, Selvaratnam, Pegallapati et al 2015, Henkanatte-Gedera, Selvaratnam et al 2016.…”

Section: Introductionmentioning

confidence: 99%

Using MinION nanopore sequencing to generate ade novoeukaryotic draft genome: preliminary physiological and genomic description of the extremophilic red algaGaldieria sulphurariastrain SAG 107.79

Davis

2016

Preprint

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We report here the de novo assembly of a eukaryotic genome using only MinION nanopore DNA sequence data by examining a novel Galdieria sulphuraria genome: strain SAG 107.79. This extremophilic red alga was targeted for full genome sequencing as we found that it could grow on a wide variety of carbon sources and could uptake several precious and rare-earth metals, which places it as an interesting biological target for disparate industrial biotechnological uses. Phylogenetic analysis clearly places this as a species of G. sulphuraria. Here we additionally show that the genome assembly generated via nanopore long read data was of a high quality with regards to low total number of contiguous DNA sequences and long length of assemblies. Collectively, the MinION platform looks to rival other competing approaches for de novo genome acquisition with available informatics tools for assembly. The genome assembly is publically released as NCBI BioProject PRJNA330791. Further work is needed to reduce small insertion-deletion errors, relative to short-read assemblies.-

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Evaluation of a thermo-tolerant acidophilic alga, Galdieria sulphuraria, for nutrient removal from urban wastewaters

Cited by 103 publications

References 15 publications

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens

Eisosome Ultrastructure and Evolution in Fungi, Microalgae, and Lichens

The Red MicroalgaGaldieriaas a Promising Organism for Applications in Biotechnology

Using MinION nanopore sequencing to generate ade novoeukaryotic draft genome: preliminary physiological and genomic description of the extremophilic red algaGaldieria sulphurariastrain SAG 107.79

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