2022
DOI: 10.14202/vetworld.2022.2772-2784
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Evaluation of a vaccine candidate isolated from Cryptosporidium parvum oocyst in mice

Abstract: Background and Aim: Cryptosporidiosis is a leading cause of diarrheal disease worldwide and is an animal and public health burden. This study aimed to evaluate the protective potential of affinity-purified Cryptosporidium parvum oocyst antigen as a vaccine candidate according to fecal oocyst shedding, humoral and cellular immune responses, histopathological changes, and the number of parasite developmental stages in ileal and hepatic tissues. Materials and Methods: We isolated oocysts from naturally infected … Show more

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Cited by 5 publications
(14 citation statements)
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“…In this study, NF-κB expression was lower in the immunized C. parvum-infected ileal and liver mice tissues than in the infected mice tissues, which might indicate the protective effect of our candidate vaccine against in ammation. This is consistent with our previous study, where we showed that immunized C. parvum-infected tissues displayed a slight pathogenic effect than nonimmunized infected tissues [32]. This also agreed with Shang et al [52], who found that vaccination suppressed NF-κB expression in the mucosal epithelium tissues and hence, blocked the in ammatory response.…”
Section: Discussionsupporting
confidence: 93%
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“…In this study, NF-κB expression was lower in the immunized C. parvum-infected ileal and liver mice tissues than in the infected mice tissues, which might indicate the protective effect of our candidate vaccine against in ammation. This is consistent with our previous study, where we showed that immunized C. parvum-infected tissues displayed a slight pathogenic effect than nonimmunized infected tissues [32]. This also agreed with Shang et al [52], who found that vaccination suppressed NF-κB expression in the mucosal epithelium tissues and hence, blocked the in ammatory response.…”
Section: Discussionsupporting
confidence: 93%
“…We observed high expression of both CD4 and CD8 antibodies in immunized infected mice, where the CD4 counts were back to being higher than CD8 in both organs. This nding, combined with lower oocyst shedding and pathological lesions in the immunized infected mice than the infected nonimmunized ones on the 10th day post-infection (as previously shown by our study: Aboelsoued et al [32], might suggest the protective effect of the vaccine candidate. Tissue damage at parasitic infection sites indicates host cell death or apoptosis in response to an intracellular pathogen, such as C. parvum [18,19].…”
Section: Discussionsupporting
confidence: 89%
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