Evaluation of Advanced Sewage Treatment Processes for Reuse of Wastewater Using Bioassays

Shishida, Kenichi; Echigo, Shinya; Kosaka, Koji; Tabasaki, M.; Matsuda, T.; Takigami, Hidetaka; Yamada, Haruo; Shimizu, Yasutoshi; Matsui, Saburo

doi:10.1080/09593332108618090

Cited by 17 publications

(8 citation statements)

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“…Moreover, these sites repel negatively charged matrices, thus further reducing interferences. This is highly advantageous over solid-phase extractions of steroid hormones from environmental matrixes using either octadecyl (C18)-bonded silica adsorbent − or HLB cartridge. Extracts of C18 or HLB often require further clean up by liquid–liquid extraction, solid phase purification on C18/NH2 columns, , silica gel column chromatography, , gel permeation on Biobeads SX-3 columns, high performance liquid chromatography (HPLC) fractionation, , or a combinations of these methods. ,, Intense extraction and clean up procedures increase the potential for mechanical losses of the analyte of interest …”

Section: Resultsmentioning

confidence: 99%

Quantification of a Male Sea Lamprey Pheromone in Tributaries of Laurentian Great Lakes by Liquid Chromatography–Tandem Mass Spectrometry

Johnson

Brant

et al. 2011

Environ. Sci. Technol.

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We developed an assay for measuring 7α,12α,24-trihydroxy-5a-cholan-3-one-24-sulfate (3kPZS), a mating pheromone released by male sea lampreys (Petromyzon marinus), at low picomolar concentrations in natural waters to assess the presence of invasive populations. 3kPZS was extracted from streamwater at a rate of recovery up to 90% using a single cation-exchange and reversed-phase mixed-mode cartridge, along with [(2)H(5)]3kPZS as an internal standard, and quantified using ultrahigh performance liquid chromatography-tandem mass spectrometry. The limit of detection was below 0.1 ng L(-1) (210 fM), which was the lowest concentration tested. Intra- and interday coefficients of variation were between 0.3-11.6% and 4.8-9.8%, respectively, at 1 ng 3kPZS L(-1) and 5 ng 3kPZS L(-1). This assay was validated by repeat measurements of water samples from a stream spiked with synthesized 3kPZS to reach 4.74 ng L(-1) or 0.24 ng L(-1). We further verified the utility of this assay to detect spawning populations of lampreys; in the seven tributaries to the Laurentian Great Lakes sampled, 3kPZS concentrations were found to range between 0.15 and 2.85 ng L(-1) during the spawning season in known sea lamprey infested segments and were not detectable in uninfested segments. The 3kPZS assay may be useful for the integrated management of sea lamprey, an invasive species in the Great Lakes where pheromone-based control and assessment techniques are desired.

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Section: Resultsmentioning

confidence: 99%

Quantification of a Male Sea Lamprey Pheromone in Tributaries of Laurentian Great Lakes by Liquid Chromatography–Tandem Mass Spectrometry

Johnson

Brant

et al. 2011

Environ. Sci. Technol.

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“…From the amount of flowing river water and the volume of the effluent at this sampling point, 30% of the river water is estimated to be of effluent origin (). Sewage-treatment plants are known to be the major source of E1 and E2 (i.e., human/animal urine origin) in river water ( − ). In the Katsura River, an administrative agency's survey detected E1 and E2 with comparable concentrations to ours using an enzyme-linked immunosorbent assay and concluded that the major source is effluent from the sewage-treatment plant ().…”

Section: Resultsmentioning

confidence: 99%

Detection of Genistein as an Estrogenic Contaminant of River Water in Osaka

Kawanishi

Takamura-Enya

Ermawati

et al. 2004

Environ. Sci. Technol.

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The estrogenic activity in water at various localities on Lake Biwa-Yodo River, a representative watershed in Japan, was measured using a recombinant yeast that expresses the human estrogen receptor. The yeast bioassay revealed that the activities of 13 water samples had an average value of 14 pmol/L (3.8 ng/L) (17beta-estradiol equivalent) with a very wide range from 0 to 72 pmol/L (0-19.6 ng/ L), and two of the samples had prominent levels of activity (72 pmol/L (19.6 ng/L) and 56 pmol/L (15.2 ng/L)). We analyzed these two samples with instrumental approaches. A high-performance liquid chromatogram profile showed that the strong activity in one sample, which was collected just downstream of a sewage-treatment plant, would be due to 17beta-estradiol and estrone, whose source is considered to be human urine contained in the effluent of the plant. The activity in the other sample, which was obtained from a tributary river in a primarily residential area with some industrial development (i.e., Osaka City), however, did not correspond to 17beta-estradiol, estrone, or synthetic chemicals known as estrogenic. Analysis of a fraction with estrogenic activity by liquid chromatography-mass spectrometry (LC-MS) provided evidence that the activity in the water sample resulted from the presence of genistein, an isoflavone compound of plant origin.

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“…This may be explained by the presence of calcium-sensitive channels on the cell membrane which have a role as gravireceptors (Häder et al 2009). Shishida et al (2000) have tested the quality of different sewage treatment processes of wastewater by means of bioassays. Lake Mariout plays an important economic role in aquatic ecology.…”

Section: Discussionmentioning

confidence: 99%

Monitoring of Waste Water Samples Using the ECOTOX Biosystem and the Flagellate Alga Euglena gracilis

Ahmed

Häder²

2010

Water Air Soil Pollut

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In this study, a fast bioassay using the ECOTOX system to evaluate biological safety of waste water samples from different sources was performed. This biological system works full automatically to test water quality. The system uses the image analysis of movement behavior of the flagellate Euglena gracilis as a model organism. The measured parameters are cell motility, velocity, orientation (r-value, upward swimming and alignment) as well as cell form (compactness). In most tested waste samples, precision of orientation (r-value) is inhibited at concentrations which showed less effect on the other parameters. Motility and compactness were noticed to be the least inhibited parameters in response to waste water samples. The results of waste treatment plants revealed the efficiency of the used purification system. Movement as well as orientation parameters in E. gracilis showed high sensitivity toward chlorine. The order of sensitivity was motility>velocity> r-value>upward swimming with EC 50 values of 0.69, 0.81, 0.85, and 1.78 mgL −1 , respectively. ECOTOX with its test organism (E. gracilis) provides an automatic, fast and sensitive system to monitor water samples.

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Evaluation of Advanced Sewage Treatment Processes for Reuse of Wastewater Using Bioassays

Cited by 17 publications

References 0 publications

Quantification of a Male Sea Lamprey Pheromone in Tributaries of Laurentian Great Lakes by Liquid Chromatography–Tandem Mass Spectrometry

Quantification of a Male Sea Lamprey Pheromone in Tributaries of Laurentian Great Lakes by Liquid Chromatography–Tandem Mass Spectrometry

Detection of Genistein as an Estrogenic Contaminant of River Water in Osaka

Monitoring of Waste Water Samples Using the ECOTOX Biosystem and the Flagellate Alga Euglena gracilis

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