Evaluation of Agar-Based Medium with Sheep Sera for Testing of Drug Susceptibility of Mycobacterium tuberculosis to Isoniazid, Rifampin, Ethambutol, and Streptomycin

Çoban, Ahmet Yılmaz; Uzun, Meltem; Bozdoğan, Bülent

doi:10.1128/jcm.01947-13

Cited by 5 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning isolation, routine sheep blood medium sustains the isolation and growth of the vast majority of mycobacteria encountered in clinical microbiology laboratories, including M. tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare (24,141,142), with the notable exception of M. leprae, which cannot be propagated outside animals (143) but can be cultured in the footpads of immunocompromised mice. In addition, sheep blood agar and sheep serum agar media have also been used to analyze in vitro susceptibility to antibiotics (144). Incubation temperature is another key factor for the successful culture of mycobacteria.…”

Section: Mycobacteriamentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

View full text Add to dashboard Cite

SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

show abstract

Section: Mycobacteriamentioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

View full text Add to dashboard Cite

show abstract

“…This may have been a mistake because the time to detection routinely achieved is usually longer than the 1 week reported by R. Koch. The chance observation that sheep‐blood agar supported the growth of M. tuberculosis led to a renewed interest in media containing sheep blood and recently, a sheep serum‐agar medium was reported for testing M. tuberculosis antibiotic susceptibility . We recommend that the basic R. Koch medium is not forgotten.…”

Section: R Koch's Lessonmentioning

confidence: 99%

Steps towards the discovery of Mycobacterium tuberculosis by Robert Koch, 1882

Cambau

Drancourt

2014

Clinical Microbiology and Infection

123

View full text Add to dashboard Cite

Palaeomicrobiology has detected the tuberculosis agent in animal and human skeletons that are thousands of years old. The German doctor Robert Koch was the first microbiologist to report in 1882 the successful isolation of the causative agent of tuberculosis, named 1 year later as Mycobacterium tuberculosis. This immense discovery, however, was not made from scratch, but involved the combining of previous scientific knowledge, chiefly the previous demonstration by the French doctor Jean-Antoine Villemin that tuberculosis was a transmissible disease, and two innovations--a new staining procedure that allowed R. Koch to consistently observe the new organism in tuberculous lesions, and use of a solidified, serum-based medium instead of broths for the culture. These innovations allowed R. Koch not only to isolate M. tuberculosis from animal and patient specimens for the first time, but also to reproduce the disease in experimentally inoculated guinea pigs. It is thanks to R. Koch that one of the most lethal diseases in human history could be diagnosed, could be treated and cured after the discovery of streptomycin 65 years later, and could be efficiently prevented by isolation of cases. His microbiological innovations are now being renewed with molecular and improved culture-based detection being the twenty-first century weapons in the fight against this disease, which remains a major killer.

show abstract