1999
DOI: 10.1016/s0887-2333(99)00037-5
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Evaluation of Alamar Blue Reduction for the In Vitro Assay of Hepatocyte Toxicity

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Cited by 43 publications
(26 citation statements)
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“…Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a measure of the viable cells (Ahmed et al, 1994;Slaughter et al, 1999;Zhi-Jun et al,1997).…”
Section: Determination Of Viability and Metabolic Activity With Resarmentioning
confidence: 99%
“…Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a measure of the viable cells (Ahmed et al, 1994;Slaughter et al, 1999;Zhi-Jun et al,1997).…”
Section: Determination Of Viability and Metabolic Activity With Resarmentioning
confidence: 99%
“…Parallel cultures were prepared in 6-well plates for spectroscopic assay and investigation of Alamar Blue (AB) is a resazurin dye [29] (dark blue, non-fluorescent) which is reduced to resorufin (pink and highly fluorescent) through a redox reaction, the level of which is indicative of cellular proliferation [29,30]. Throughout this study, the pink, fluorescent molecule resorufin was excited at 540 nm and the fluorescence read at 595 nm.…”
Section: Fluorescence and Absorbance Assaysmentioning
confidence: 99%
“…To avoid misinterpretation of the results, YO-PRO-1 fluorescence signal has to be monitored on cells with preserved membrane integrity wherein YO-PRO-1 can only enter through activated P2X7 receptor [48]. Neutral red uptake assay for the estimation of cell viability through measurement of membrane integrity is highly recommended [49], but other cell viability assays can be used such as the Alamar Blue assay [50] or the MTT assay [51].…”
Section: Preparation Of Samplesmentioning
confidence: 99%