Evaluation of alfaxalone and midazolam with or without flumazenil reversal in Egyptian fruit bats (Rousettus aegyptiacus)

Tuval, Avishag; Dror-Maman, Inbal; Las, Liora; Bdolah‐Abram, Tali; Shilo‐Benjamini, Yael

doi:10.1016/j.vaa.2020.12.002

Cited by 6 publications

(14 citation statements)

References 32 publications

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“…Indeed, in this study, HR was significantly lower in DK group than DBM group. Comparing baseline values of RR reported by Tuval and colleagues (2018), in the present study both protocols showed a slight reduction in the values of this physiological parameter if compared to other studies [21,34]. Bats in group DBM showed the greatest reduction in RR during anaesthesia, being significantly lower than which occurred in group DK.…”

Section: Discussionsupporting

confidence: 55%

“…In the present study, baseline values in manually restrained bats before drugs administration were not measured. However, physical restraint is stressful and would have altered values themselves, as reported in previous studies [34]. Therefore, considering the resting parameters in the literature, it is possible to assume that there was a decrease in HR following the administration of both injectable protocols and this finding is probably imputable to the effect of DEX.…”

Section: Discussionmentioning

confidence: 75%

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Comparison of two injectable anaesthetic protocols in Egyptian fruit bats(Rousettus aegyptiacus)undergoing gonadectomy

Amari

Brioschi

Rabbogliatti

et al. 2021

Preprint

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Egyptian fruit bats are experimental animals of increasing interest because they have been identified as a natural reservoir for several emerging zoonotic viruses. For this reason, bats could undergo different experimental procedures that require sedation or anaesthesia. Our aim was to compare the effects of two balanced anaesthetic protocols on sedation, cardiopulmonary variables and recovery in bats undergoing gonadectomy. Twenty bats were randomized into two groups; patients in group DK received intramuscular injection of dexmedetomidine (40 μg kg-1) and ketamine (7 mg kg-1), whereas those in group DBM were anaesthetized with intramuscular dexmedetomidine (40 μg kg-1), butorphanol (0.3 mg kg-1) and midazolam (0.3 mg kg-1). Time of induction, cardiopulmonary parameters and anaesthetic depth were measured. If anaesthesia plan was considered inadequate, fraction of inspired isoflurane was titrate-to-effect to achieve immobility. At the end of the surgery venous blood gas analysis was performed and intramuscular atipamezole (200 μg kg-1) or atipamezole (200 μg kg-1) and flumazenil (0.03 mg kg-1) was administered for timed and scored recovery phase. A significantly higher heart rate and peripheral oxygen saturation were recorded in DBM group (p = 0.001; p = 0.003 respectively), while respiratory rate was significantly lower than DK group (p = 0.001). All bats required isoflurane supplementation during surgery with no significant difference. No differences were observed in rectal temperature, induction and recovery times. Sodium and chlorine where significantly higher in DBM group (p = 0.001; p = 0.002 respectively). Recovery scores in group DK were significantly better than in group DBM (p = 0.034). Both protocols induced anaesthesia in Egyptian fruit bats with comparable sedative and cardiorespiratory effect. These drug combinations may be useful for minor procedures in bats, and they could be associated with inhalation anaesthesia in determining and maintaining a surgical anaesthetic plan.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 75%

Comparison of two injectable anaesthetic protocols in Egyptian fruit bats(Rousettus aegyptiacus)undergoing gonadectomy

Amari

Brioschi

Rabbogliatti

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In the present study, baseline values in manually restrained bats before drugs administration were not measured. However, physical restraint is stressful and would have altered values themselves, as reported in previous studies 30 . Therefore, considering the resting parameters in the literature, it is possible to assume that there was a decrease in HR following the administration of both injectable protocols and this finding is probably imputable to the effect of DEX.…”

Section: Discussionmentioning

confidence: 53%

“…Depth of anaesthesia was assessed every 10 min by easy extension and flexion of the wing without any voluntary movement 29 or presence of muscle tone by opening the jaw 30 . In case of spontaneous movement or presence of muscle tone, the anaesthesia depth was considered inadequate, and the fraction of inspired isoflurane (FI-ISO) was titrated-to-effect to the minimum concentration to achieve immobility and loss of muscle tone and this value was recorded and then adjusted over time as needed.…”

Section: Methodsmentioning

confidence: 99%

Comparison of two injectable anaesthetic protocols in Egyptian fruit bats (Rousettus aegyptiacus) undergoing gonadectomy

Amari

Brioschi

Rabbogliatti

et al. 2022

Sci Rep

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Egyptian fruit bats have gained increasing interest being a natural reservoir for emerging zoonotic viruses. Anaesthesia is often required to allow safe handling of bats. We aimed to compare the sedative and cardiopulmonary effects of two balanced anaesthetic protocols in bats undergoing gonadectomy. Group DK (n = 10) received intramuscular dexmedetomidine (40 µg/kg) and ketamine (7 mg/kg), whereas group DBM (n = 10) received intramuscular dexmedetomidine (40 µg/kg), butorphanol (0.3 mg/kg) and midazolam (0.3 mg/kg). Induction time and cardiopulmonary parameters were recorded. If anaesthetic plan was inadequate, isoflurane was titrated-to-effect. At the end of surgery venous blood gas analysis was performed and atipamezole or atipamezole-flumazenil was administered for timed and scored recovery. In DBM group heart rate and peripheral oxygen saturation were significantly higher (p = 0.001; p = 0.003 respectively), while respiratory rate was significantly lower (p = 0.001). All bats required isoflurane supplementation with no significant differences between groups. Induction and recovery times showed no significant differences. In group DK a better recovery was scored (p = 0.034). Sodium and chloride were significantly higher in DBM group (p = 0.001; p = 0.002 respectively). Both anaesthetic protocols were comparable and can be recommended for minor procedures in bats.

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“…Flumazenil at 0.05, 0.1 and 0.2 mg/kg IM successfully diminish a period of alfaxalone anesthesia in the chick's by18, 22 and 41%, respectively. Flumazenil also reverse the anesthetic effect of alfaxalone and midazolam in Egyptian fruit bats [34]. The possible mechanism of the flumazenil by which reduce the duration of general anesthesia is the inverse agonist activity of the GABAA receptor in the central nervous system [26,33,35,36].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Anesthetic Action of Alfaxalone in Chicks and Compared with Alfaxalone/ketamine or Alfaxalone/xylazine.

alatrushi¹,

Naser²

2021

NIDOC-ASRT

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O BJECTIVE, alfaxalone is a neuro-steroidal anesthetic agent. The data on the anesthetic properties of alfaxalone in the birds is relatively inadequate. This article studies the anesthetic effect and efficiency of alfaxalone /ketamine or alfaxalone /xylazine anesthesia given in 7-10 day-old chicks. Methods, We used the up and down method to determine the median effective anesthetic dose of alfaxalone, time to onset of anesthesia, duration of action, temperature, respiratory rate, and antagonize with flumazenil were evaluated. Results, The median effective anesthetic dose (ED 50 ) of alfaxalone was 32.88mg/kg . intraperitoneally. Alfaxalone at 25, 50, and 100 mg/kg induced anesthesia in chicks for (10 -48 minutes). Flumazenil decreased the anesthetic period of alfaxalone. Alfaxalone at 50mg/kg causes bradypnea whereas at 100 mg/kg causes tachypnea. Alfaxalone produce hypothermia. The duration of anesthesia was significantly longer in alfaxalone/xylazine than in alfaxalone/ketamine but it causes more bradypnea. Conclusion, alfaxalone produces light surgical anesthesia so that it can be mixed with ketamine or xylazine for deep surgical anesthesia. Flumazenil may reverse the anesthetic effect of alfaxalone.

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Evaluation of alfaxalone and midazolam with or without flumazenil reversal in Egyptian fruit bats (Rousettus aegyptiacus)

Cited by 6 publications

References 32 publications

Comparison of two injectable anaesthetic protocols in Egyptian fruit bats(Rousettus aegyptiacus)undergoing gonadectomy

Comparison of two injectable anaesthetic protocols in Egyptian fruit bats(Rousettus aegyptiacus)undergoing gonadectomy

Comparison of two injectable anaesthetic protocols in Egyptian fruit bats (Rousettus aegyptiacus) undergoing gonadectomy

Evaluation of the Anesthetic Action of Alfaxalone in Chicks and Compared with Alfaxalone/ketamine or Alfaxalone/xylazine.

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