Evaluation of allergenicity of genetically modified soybean protein extract in a murine model of oral allergen‐specific sensitization

Gizzarelli, F; Corinti, Silvia; Barletta, Bianca; Iacovacci, Patrizia; Brunetto, Barbara; Butteroni, Cinzia; Afferni, Claudia; Onori, Roberta; Miraglia, Marina; Panzini, Gianluca; Felice, Gabriella Di; Tinghino, Raffaella

doi:10.1111/j.1365-2222.2005.02415.x

Cited by 66 publications

(44 citation statements)

References 50 publications

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“…In the present study, the serum IgG levels were significantly increased in 35-d-old piglets sensitised by glycinin. This indicated that glycinin peptides were absorbed into the blood and produced large quantities of IgG antibody in the serum, and they played an important role in the systemic immune response, which had been verified in previous studies (Helm et al 2002;Gizzarelli et al 2006).…”

Section: Discussionsupporting

confidence: 72%

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Cao

Meng

et al. 2016

Italian Journal of Animal Science

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Glycinin, an antigenic glycoprotein found in soybeans, is the major cause of allergic reactions in young animals. The objective of this study was to evaluate the effects of a prior immunisation of sucking piglets with glycinin on their post-weaning growth performance, serum immunoglobulin contents, small intestinal histamine release and mucosal histology. Forty piglets (7 d of age) were randomly divided into four groups of 10 piglets each. Piglets of Group C (Control) received a physiological saline solution, Groups Im (Immunised) and Im þ S (Sensitised) were immunised twice with 500 lg/kg of glycinin at 7 and 21-d-old. All piglets were weaned at 23 d; Groups Im þ S and S were sensitised with 2500 lg/kg of glycinin at weaning. Compared with Group C, in Group S the average daily gain (ADG), average daily feed intake and gain:feed ratio were decreased, and serum levels of IgG and IgE were increased. Furthermore, in this group, the histamine levels in the duodenum and ileum were significantly decreased, and the structure of duodenal and ileal mucosa was severely damaged. On the contrary, in Groups Im and Im þ S the ADG was increased, serum IgE levels were decreased, intestinal histamine levels were increased and the intestinal mucosa was not damaged. These findings suggest that prior immunisation with glycinin can protect the structural integrity of the intestinal mucosal epithelia and alleviate allergic reactions in piglets.ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 72%

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Cao

Meng

et al. 2016

Italian Journal of Animal Science

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“…Thus assessment of the immunoglobulin levels after antigen administration allows determination of allergenic potential. Gizzarelli et al (2006) showed that sensitization with both GE and wild-type soybean extracts induced high levels of antigen-specific IgE, IgG1 and low levels of specific IgG2a in Balb/c mice. Singh et al (2006) observed comparable immune response (IgE and IgG1) for transgenic and wild-type mustard in Balb/c mice.…”

Section: Discussionmentioning

confidence: 98%

Safety assessment of leaf curl virus resistant tomato developed using viral derived sequences

et al. 2009

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Genetic engineering of food crops has significantly influenced the agricultural productivity over the past two decades. It has proved a valuable tool, offering crops with higher yields, improved nutritional quality, resistance against pesticides, herbicides and tolerance against abiotic stresses. However, the safety assessment of genetically engineered (GE) crops is prerequisite before introduction into human food chain. The present study was aimed to assess the toxicity and allergenicity of leaf curl virus resistant GE tomato compared to its wild-type species. Balb/c mice fed with genetically engineered or wild-type tomato did not show significant differences in growth, body weight (P > 0.05) and food consumption when compared with control mice. Values for serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminase, urea and cholesterol were comparable in GE and wild-type tomato fed mice. Mice immunized with GE or wild-type tomato extract showed low IgE response. Lung histology of ovalbumin fed mice showed bronchoconstriction with eosinophilic infiltration whereas GE or wild-type tomato showed no cellular infiltration with normal airways. Genetically engineered and wild-type tomato sensitized mice demonstrated similar IL-4 release in splenic cell culture supernatant. GE and wild tomato extract on ELISA showed comparable IgE binding (P > 0.05) with food allergic patients' sera. In conclusion, genetically engineered tomato showed no toxicity in mice and allergenicity is similar to the wild-type tomato.

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“…The levels of β-conglycinin-specific IgE were measured by indirect ELISA following the procedures described by Gizzarelli et al [34] with slight modifications. Immulon II 96-well microplates (Dynatech Laboratories, Chantilly, VA) were coated with 20 μg/mL purified β-conglycinin in coating buffer (Sigma, St Louis, MO) and incubated for 3 h at 37°C.…”

Section: Measurement Of Serum β-Conglycinin-specific Igementioning

confidence: 99%

Saikosaponin-d inhibits β-conglycinin induced activation of rat basophilic leukemia-2H3 cells

Hao

Piao

Piao³

2012

International Immunopharmacology

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Evaluation of allergenicity of genetically modified soybean protein extract in a murine model of oral allergen‐specific sensitization

Cited by 66 publications

References 50 publications

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Induction of immune responses and allergic reactions in piglets by injecting glycinin

Safety assessment of leaf curl virus resistant tomato developed using viral derived sequences

Saikosaponin-d inhibits β-conglycinin induced activation of rat basophilic leukemia-2H3 cells

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