Evaluation of alternative measurement and data-processing options for enzyme-based biosensors

Chen, Weibin; Pardue, Harry L.; Williams, Sheldon

doi:10.1016/s0003-2670(99)00078-1

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Instrumentation Automation and Data Processing For Kinetic M...1

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2005

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“…Workers have continued to study and assess data processing approaches for kinetic measurements and determinations. Options for processing data from enzyme-based biosensors have been evaluated (38). The authors recommend a pseudoequilibrium approach where the response corresponding to reaction of all the substrate in a fixed volume of solution is obtained from fitting a model.…”

Section: Instrumentation Automation and Data Processing For Kinetic M...mentioning

confidence: 99%

Kinetic Determinations and Some Kinetic Aspects of Analytical Chemistry

2000

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Section: Instrumentation Automation and Data Processing For Kinetic M...mentioning

confidence: 99%

Kinetic Determinations and Some Kinetic Aspects of Analytical Chemistry

2000

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Extended-range glucose biosensor via layer-by-layer assembly incorporating gold nanoparticles

Zhao¹

2005

Front Biosci

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We report on a glucose oxidase (GOx)/polyelectrolyte (PE)/gold nanoparticle (AuNP) multilayer films that can be utilized as efficient glucose biosensors by layer-by-layer self-assembly method. Electrochemical impedance spectroscopy (EIS) and UV-visible spectroscopy were adopted to monitor the regular growth of the multilayer films. The role of gold nanoparticles integrated in the multilayer films not only increase the amount and activity of GOx, but also significantly improve the electron-transfer characteristics of the films. The performance of the multilayer films for sensing glucose could be tailored by controlling the gold nanoparticles loading in the film and the number of layers. A biosensor constructed by four poly(dimethyldiallylammonium chloride) (PDDA)/AuNP/PDDA/GOx multilayer films exhibited a wide linear calibration range from 0 to 60.0 mM with the detection limit of 3.0 microM for the detection of glucose. The dynamic range can be extended up to 120 mM. The biosensor has good stability and reproducibility.

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Evaluation of alternative measurement and data-processing options for enzyme-based biosensors

Cited by 2 publications

References 10 publications

Kinetic Determinations and Some Kinetic Aspects of Analytical Chemistry

Kinetic Determinations and Some Kinetic Aspects of Analytical Chemistry

Extended-range glucose biosensor via layer-by-layer assembly incorporating gold nanoparticles

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