Evaluation of Amino Acids Profile as Non-Invasive Biomarkers of Hepatocellular Carcinoma in Egyptians

Ghanem, Samar; Abdel‐Samiee, Mohamed; El-Said, Hala; Youssef, Mohamed; Elzohry, Hassan; Abdelsameea, Eman; Moaz, Inas; Abdelwahab, Sayed F.; Elaskary, Shymaa A.; Zaher, Eman Mohammed; Helal, Marwa

doi:10.3390/tropicalmed7120437

Cited by 6 publications

(6 citation statements)

References 39 publications

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“…The relative importance of both the kynurenine pathway and ADMA was assessed with the kynurenine/tryptophan ratio and the Arg/ADMA ratio. Finally, the Fisher's ratio (defined as the sum of BCAAs, Leu, Ile, and Val divided by the sum of aromatic AAs, Tyr, and Phe) and the GSG index [Glu/(Ser + Gly)] were also calculated, as they have been observed to be altered in several pathologies and metabolic dysfunctions [13,14].…”

Section: Sample Collection and Amines Analyzedmentioning

confidence: 99%

Prognostic Significance of Amino Acid and Biogenic Amines Profiling in Chronic Kidney Disease

Gervasini,

Verde,

González

et al. 2023

Biomedicines

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There is a pressing need for more precise biomarkers of chronic kidney disease (CKD). Plasma samples from 820 subjects [231 with CKD, 325 with end-stage kidney disease (ESKD) and 264 controls] were analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) to determine a metabolic profile of 28 amino acids (AAs) and biogenic amines to test their value as markers of CKD risk and progression. The kynurenine/tryptophan ratio showed the strongest correlation with estimated glomerular filtration rate values (coefficient = −0.731, p < 0.0001). Models created with orthogonal partial least squares-discriminant analysis (OPLS-DA) containing the metabolic signature showed a high goodness of fit and predictability for controls/CKD (R2X:0.73:R2Y:0.92:Q2:0.92, p < 0.0001) and lower values for CKD/ESKD (R2X:0.56:R2Y:0.59:Q2:0.55, p < 0.0001). Based on generated VIP scores, the most relevant markers for segregating samples into control/CKD and CKD/ESKD groups were citrulline (1.63) and tryptophan (1.47), respectively. ROC analysis showed that the addition of the metabolic profile to a model including CKD classic risk factors improved the AUC from 86.7% (83.6–89.9) to 100% (100–100) for CKD risk (p < 0.0001) and from 63.0% (58.2–67.8) to 96.5% (95.3–97.8) for the risk of progression from CKD to ESKD (p < 0.0001). Plasma concentrations of AAs and related amines may be useful as diagnostic biomarkers of kidney disease, both for CKD risk and for progression of CKD patients to ESKD.

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Section: Sample Collection and Amines Analyzedmentioning

confidence: 99%

Prognostic Significance of Amino Acid and Biogenic Amines Profiling in Chronic Kidney Disease

Gervasini,

Verde,

González

et al. 2023

Biomedicines

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“…A potential factor that might contribute to this phenomenon is the downregulation of the immune response after effective DAA treatment, which may increase susceptibility to the formation of neoplasia. However, more investigation via larger multicenter trials is necessary to validate these findings 30 …”

Section: Introductionmentioning

confidence: 99%

“…However, more investigation via larger multicenter trials is necessary to validate these findings. 30 There is a possibility that idiopathic pulmonary fibrosis (IPF) might be classified as an EHM, given the notable incidence of HCV antibodies in individuals affected by this condition. It is noteworthy that therapy-naive individuals with HCV had an elevated prevalence of chronic alveolitis based on the results from alveolar lavage.…”

Section: Introductionmentioning

confidence: 99%

A multicentric and nationwide predictive study role of T cell sub‐population in the prevalence and prognosis of cryoglobulinemia among genotype 4 chronic hepatitis C patients

Abdel‐Samiee,

Youssef,

Elghamry

et al. 2023

Journal of Medical Virology

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The infection caused by the hepatitis C virus (HCV) is a significant global health concern. The prevailing genotype of HCV in Egypt is 4a, commonly referred to as GT‐4a. A significant proportion exceeding 50% of patients infected with HCV experience extrahepatic manifestations (EHMs), encompassing a diverse range of clinical presentations. These manifestations, including essential mixed cryoglobulinemia (MC), can serve as initial and solitary indicators of the disease. The complete understanding of the pathogenesis of EHM remains unclear, with autoimmune phenomena being recognized as the primary causative factor. In this study, we examined the predictive significance of T‐cell subpopulations in relation to the occurrence and prognosis of cryoglobulinemia in HCV patients. A total of 450 CHC genotype four treatment naïve patients were enrolled in this analytic cross‐sectional study after thorough clinical, laboratory, and radiological examinations. All patients underwent laboratory investigations, including testing for cryoglobulin antibodies and measurements of CD4 and CD8 levels; two groups were described according to their test results: Group 1 consists of patients who have tested positive for cryoglobulin antibodies and Group 2 consists of patients who have tested negative for cryoglobulin antibodies. The exclusion criteria encompassed individuals with HIV infection or chronic HBV infection. Additionally, pelvi‐abdominal ultrasonography was performed. Our study included 450 treatment naïve CHC patients (59% male, mean age 50.8 years). The patients were categorized according to their cryoglobulin antibodys test results into two groups: group A, CHC patients with cryoglobulin antibodies (Abs) negative (364 patients), and group B, CHC patients with cryoglobulin Ab positive (86 patients). Group B demonstrated a higher average age, elevated international normalized ratio, more prolonged duration of HCV infection, lower albumin, higher alanine aminotransferase, higher aspartate aminotransferase, higher bilirubin, lower CD8, lower CD4, and lower CD4:CD8 ratio. In contrast, 27 out of 86 (31.40%) patients in group B had symptoms; 85.8% had purpura and arthralgia, 74.3% had paresthesias, 86.7% had weakness, and 12.2% had non‐Hodgkin's lymphoma. The levels of CD4 and CD8 were found to be decreased in chronic HCV patients with MC. T‐cell subpopulation serves as a reliable indicator for assessing the prevalence and prognosis of MC in individuals with genotype 4 chronic hepatitis C. However, additional research is needed to further understand the development and spread of various emerging infectious diseases. Nevertheless, it is noteworthy that a critical threshold may exist beyond which EHM reaches a point of no return.

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“…Hepatocellular carcinoma (HCC) is a widespread problem worldwide, and variations in its epidemiological data across different geographical regions have been identified. Furthermore, it ranks as the fourth most prevalent type of cancer in Egypt and the sixth globally [ 1 , 2 ]. With a population of 15 million, Egypt ranks the 15 th globally and the third in Africa [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Circular RNA SMARCA5 as a Novel Biomarker for Hepatocellular Carcinoma

Bedair,

El-Banna,

Ahmed

et al. 2024

Asian Pac J Cancer Prev

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Background: Hepatocellular carcinoma (HCC) is the fourth most prevalent type of cancer in Egypt and the sixth globally. Most patients with HCC are typically diagnosed during the advanced stages of the disease due to the absence of biomarkers for early detection. Consequently, these patients miss the optimal timeframe for receiving therapy. Objective: we aimed to assess the circular RNA SMARCA5 level and SMARCA5 mRNA gene expression as a potential biomarker for early detection of HCC. Methods: The present study utilized a case-control design comprising 159 participants. Participants were selected from both inpatient and outpatient hepatology and gastroenterology clinics at the National Liver Institute Hospital, Menoufia University. They were evenly distributed among three groups: Group I: 53 control subjects, Group II: 53 HCV cirrhotic patients, and Group III: 53 HCC patients. Tumor staging was done using BCLC staging system. Each patient underwent a thorough clinical examination, radiological examination, complete history taking, and serum Alpha-fetoprotein (AFP) assessment and detection of circular RNASMARCA5 and SMARCA5mRNA gene sutilizing quantitative real-time polymerase chain reaction. Results: Statistically substantial differences were observed in the examined groups in terms of AFP, SMARCA5, and CircSMARCA5 (P-value = 0.001, 0.001 & 0.001). CircSMARCA5 and SMARCA5mRNA were markedly down regulated in the HCC group compared to HCV cirrhotic patients and controls. ROC analysis for early HCC diagnosis demonstrated that the CircSMARCA5 area under the curve (AUC) at cut-off point 4.55 yielded a specificity of 83.8% and sensitivity of 91.7%. The AUC for AFP at a cut-off point of 515ng/ml yielded a specificity of 89.2% and a sensitivity of 91.3%. Conclusion: CircSMARCA5 has the potential to be a more sensitive predictor of HCC disease compared to AFP.

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Evaluation of Amino Acids Profile as Non-Invasive Biomarkers of Hepatocellular Carcinoma in Egyptians

Cited by 6 publications

References 39 publications

Prognostic Significance of Amino Acid and Biogenic Amines Profiling in Chronic Kidney Disease

Prognostic Significance of Amino Acid and Biogenic Amines Profiling in Chronic Kidney Disease

A multicentric and nationwide predictive study role of T cell sub‐population in the prevalence and prognosis of cryoglobulinemia among genotype 4 chronic hepatitis C patients

Evaluation of Circular RNA SMARCA5 as a Novel Biomarker for Hepatocellular Carcinoma

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