Evaluation of an antigencapture enzyme immunoassay for detection ofEntamoeba histolytica in stool samples

Jelı́nek, Tomáš; Peyerl, G.; Löscher, Thomas; Nothdurft, Hans Dieter

doi:10.1007/bf01691966

Cited by 17 publications

(13 citation statements)

References 5 publications

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“…Jelinek et al reported that the sensitivity and specificity of the ProSpecT ELISA were 73.5 and 97.7%, respectively, compared to microscopy for E. histolytica/E. dispar in German travelers returning from vacation trips abroad (99).…”

Section: Antigen Detectionmentioning

confidence: 99%

Laboratory Diagnosis of Amebiasis

2003

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The detection of Entamoeba histolytica, the causative agent of amebiasis, is an important goal of the clinical microbiology laboratory. To assess the scope of E. histolytica infection, it is necessary to utilize accurate diagnostic tools. As more is discovered about the molecular and cell biology of E. histolytica, there is great potential for further understanding the pathogenesis of amebiasis. Molecular biology-based diagnosis may become the technique of choice in the future because establishment of these protozoa in culture is still not a routine clinical laboratory process. In all cases, combination of serologic tests with detection of the parasite (by antigen detection or PCR) offers the best approach to diagnosis, while PCR techniques remain impractical in many developing country settings. The detection of amebic markers in serum in patients with amebic colitis and liver abscess appears promising but is still only a research tool. On the other hand, stool antigen detection tests offer a practical, sensitive, and specific way for the clinical laboratory to detect intestinal E. histolytica. All the current tests suffer from the fact that the antigens detected are denatured by fixation of the stool specimen, limiting testing to fresh or frozen samples

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Section: Antigen Detectionmentioning

confidence: 99%

Laboratory Diagnosis of Amebiasis

2003

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“…A monoclonal antibody-based ELISA for the specific detection of E. histolytica antigen in fecal samples (TechLab E. histolytica II ELISA; TechLab, Blacksburg, VA, USA) is commercially available; however, this test can neither detect E. dispar nor E. moshkovskii, and it is not useful for patients with invasive amebiasis [15,17,18]. The sensitivity of coproantigen ELISA is reported to be 60-100% [13,[19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Epidemiological, clinical, and diagnostic data on intestinal infections with Entamoeba histolytica and Entamoeba dispar among returning travelers

et al. 2011

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“…Recent studies demonstrated that up to 90% of these patients are infected with the nonpathogenic species E. dispar and not E. histolytica (7,11,14,35). Because cysts of E. histolytica are morphologically similar to those of E. dispar, other techniques (e.g., PCR and ELISA for detection of specific antigens) are in use for differentiation of species from stools (8,32).…”

Section: Discussionmentioning

confidence: 99%

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

Doorn¹,

Hofwegen²,

Koelewijn³

et al. 2005

J Clin Microbiol

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A homemade enzyme-linked immunosorbent assay (ELISA) and a dipstick assay (Dipstick) for the detection of anti-Entamoeba histolytica antibodies in serum were developed and evaluated together with a commercially available latex agglutination test (LAT; Laboratoires Fumouze) for their use in serodiagnosis of amebiasis. The sensitivity of these assays was evaluated with sera from 27 patients with radiologically proven, cellulose acetate precipitation (CAP) test-positive amebic liver abscess, 7 patients with parasitologically and PCR-proven amebic colitis, and 11 patients with parasitologically and PCR-proven E. histolytica cyst passage. The sensitivities of the ELISA, Dipstick, and LAT were all 93.3% (42/45). With a combination of Dipstick and LAT, all abscess and colitis patients had at least one positive result. The specificity was assessed with 238 sera from patients with various parasitic, bacterial, viral, and fungal infectious diseases, sera containing autoimmune antibodies, and sera from healthy blood donors. The specificities of the ELISA, Dipstick, and LAT were 97.1%, 98.1%, and 99.5%, respectively. Of 61 sera from patients with PCR-proven E. dispar infection, 60 (98.4%) were negative in both Dipstick and LAT and 59 (96.7%) were negative in ELISA. Our data suggest that all three assays are sensitive serological tests. The rapid LAT and Dipstick provide fast results and can easily be applied in routine laboratories in order to facilitate the diagnosis of amebiasis.

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Evaluation of an antigencapture enzyme immunoassay for detection ofEntamoeba histolytica in stool samples

Cited by 17 publications

References 5 publications

Laboratory Diagnosis of Amebiasis

Laboratory Diagnosis of Amebiasis

Epidemiological, clinical, and diagnostic data on intestinal infections with Entamoeba histolytica and Entamoeba dispar among returning travelers

Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage

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