Evaluation of an Automated System for the Counting of Microbial Colonies

Heuser, Elisa; Becker, Karsten; Idelevich, Evgeny A.

doi:10.1128/spectrum.00673-23

Cited by 3 publications

(3 citation statements)

References 21 publications

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“…Colonies were suspended in sterile saline (0.9% NaCl) and shaken vigorously for 15 seconds. Density was adjusted to turbidity according to a 0.5 McFarland standard (equivalent to 1-5 x 108 colony forming units per milliliter (CFU/mL) for bacteria (17) with some modifications.…”

Section: Preparation Of Bacterial Inoculummentioning

confidence: 99%

Phytochemical characterization and antibacterial evaluation of crude saps from medicinal plants in Palestinian cuisine

Sawalha,

Khasib,

Mansour

et al. 2024

Canrea

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The emergence and evolution of antibiotic-resistant bacterial strains has shifted interest in herbal therapy. Plants have long been a source of anti-infective compounds. This study investigated the antibacterial activity and phytochemical composition of various Palestinian plants against five human pathogenic bacteria. Antibacterial activity was determined using the Agar Diffusion Method (ADM), as well as the Minimal Inhibitory Concentration (MIC) and Broth Microdilution (BMD) assays. The successful ADM bacterial inhibition zones employing plant extracts were Eucalyptus camaldulensis Dehn. (E. camaldulensis) 9.35 mm, Allium sativum L. (A. sativum) 8.35 mm, Ceratonia siliqua L. (C. siliqua) 7.85 mm, and Amygdalus communis L. (A. communis) 7.85 mm. The MIC50 values against the tested microorganisms varied from 5.69 to 398.5 mg/mL. Furthermore, the MIC50 values for Gram-positive bacteria varied from 5.69 to 233.88 mg/mL, whereas the values for Gram-negative bacteria ranged from 28.65 to 398.5 mg/mL. Most species of bacteria were effectively inhibited by E. camaldulensis and A. sativum extracts. For Gram-positive and Gram-negative bacteria, the MIC50 values for E. camaldulensis were 39.01-40.38 and 31.60-85.37 mg/mL, respectively. The MIC50 values for A. sativum against Gram-positive and Gram-negative bacteria were 5.69-14.85 mg/mL and 38.98-200.11 mg/mL, respectively. The phytochemicals such as flavonoids, steroids, proteins, carbohydrates, and alkaloids in varying amounts, may explain their diverse capability to inhibit bacteria. The present study showed that E. camaldulensis, A. sativum, C. siliqua and A. communis are valuable Palestinian medicinal plants that contain antibacterial agents against the tested bacterial species. However, this study serves as a foundation for further pharmaceutical studies.

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Section: Preparation Of Bacterial Inoculummentioning

confidence: 99%

Phytochemical characterization and antibacterial evaluation of crude saps from medicinal plants in Palestinian cuisine

Sawalha,

Khasib,

Mansour

et al. 2024

Canrea

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“…Consequently, the conventional SBA is considered labor-intensive, with a high inter-operator and inter-laboratories variability ( Borrow et al, 2006 ). Even if the introduction of automated colony counting represented an improvement, the conventional assay still is not applicable to test a large number of serum samples ( Necchi et al, 2017 ; Heuser et al, 2023 ). The need for an alternative high throughput SBA is derived from the awareness that antibody functionality is an important parameter to consider during vaccine efficacy assessment.…”

Section: Current Serological Assays Used For the Evaluation Of Bacter...mentioning

confidence: 99%

“…Then, the development of multiplexed OPA (MOPA) allowed the test of up to four different serotypes in the same assay plate, taking advantage of the use of different antibiotics to select resistant strains ( Kim et al, 2003 ). Furthermore, the automated colony counting tool allowed the acceleration of the process ( Heuser et al, 2023 ). The MOPA is particularly useful in the case of a polyvalent Pneumococcal Conjugate Vaccine (PCV) clinical test, which would require performing an OPA assay against several serotypes ( Romero-Steiner et al, 2006 ; Song et al, 2013 ).…”

Section: Current Serological Assays Used For the Evaluation Of Bacter...mentioning

confidence: 99%

Current challenges and improvements in assessing the immunogenicity of bacterial vaccines

Fantoni,

Boccadifuoco,

Verdirosa

et al. 2024

Front. Microbiol.

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The increase in antimicrobial-resistant bacterial strains has highlighted the need for a new vaccine strategy. The primary goal of a candidate vaccine is to prevent disease, by inducing a persistent immunologic memory, through the activation of pathogen-specific immune response. Antibody titer is the main parameter used to assess the immunogenicity of bacterial vaccine candidates and it is the most widely used as a correlate of protection. On the other hand, the antibody titer alone cannot provide complete information on all the activity mediated by antibodies which can only be assessed by functional assays, like the serum bactericidal assay and the opsonophagocytosis assay. However, due to the involvement of many biological factors, these assays are difficult to standardize. Some improvements have been achieved in recent years, but further optimizations are needed to minimize inter- and intra-laboratories variability and to allow the applicability of these functional assays for the vaccine immunogenicity assessment on a larger scale.

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Automated Bacterial Colony Counting on Agar Plate

Bunkum,

Visitsattapongse

2023

2023 15th Biomedical Engineering International Conference (BMEiCON)

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Evaluation of an Automated System for the Counting of Microbial Colonies

Abstract: Colony counting is a widely utilized technique in the field of microbiology. The accuracy and convenience of automated colony counters are essential for research and diagnostics.

Cited by 3 publications

References 21 publications

Phytochemical characterization and antibacterial evaluation of crude saps from medicinal plants in Palestinian cuisine

Phytochemical characterization and antibacterial evaluation of crude saps from medicinal plants in Palestinian cuisine

Current challenges and improvements in assessing the immunogenicity of bacterial vaccines

Automated Bacterial Colony Counting on Agar Plate

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