Evaluation of an Automated von Willebrand Factor Activity Assay in von Willebrand Disease

Trossaërt, Marc; Ternisien, Catherine; Lefrancois, Armelle; Llopis, Laura; Goudemand, Jenny; Sigaud, Marianne; Fouassier, Marc; Caron, Claudine

doi:10.1177/1076029610379848

Cited by 24 publications

(42 citation statements)

References 13 publications

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“…As expected, and also reported by others, all of the new assays (i.e. VWF:GPIbR by the HemosIL latex agglutination assay and the HemosIL AcuStar assay , the VWF:GPIbM Siemens assay and the VWF:Ab assay ) performed well and yielded results close to the old reference standard VWF:RCo. Although statistically significant and consistent in all participating laboratories, the slightly steeper regression lines for the VWF:Ab and VWF:GPIbM were clinically insignificant.…”

Section: Discussionsupporting

confidence: 89%

An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: the COMPASS‐VWF study

Szederjesi

Baronciani

Budde³

et al. 2018

Journal of Thrombosis and Haemostasis

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Background Several new assays have become available to measure von Willebrand factor (VWF) activity. The new assays appear to have improved performance characteristics compared with the old reference standard, ristocetin cofactor activity (VWF:RCo), but information is limited about how they compare with VWF:RCo and each other. Methods The von Willebrand factor Subcommittee of the International Society for Thrombosis and Haemostasis (ISTH) Scientific and Standardization Committee (SSC) designed a collaborative study involving expert laboratories from several countries to compare available tests with each other and with VWF:RCo. Eight laboratories from five countries were provided with blinded samples from normal healthy individuals and well-characterized clinical cases. Laboratories measured VWF activity using all tests available to them; data from six laboratories, not affected by thawing during transportation, are included in this study. Results All tests correlated well with VWF:RCo activity (r-values ranged from 0.963 to 0.989). Slightly steeper regression lines for VWF:Ab and VWF:GPIbM were clinically insignificant. The new assays showed improved performance characteristics. Of the commercially available assays, the VWF:GPIbR using the AcuStar system was the most sensitive and could reliably detect VWF activity below 1 IU dL . The lower limit of the measuring interval for the VWF:GPIbM and the VWF:GPIbR assays was in the 3-4 and 3-6 IU dL range, respectively. Inter-laboratory variation was also improved for most new assays. Conclusion All VWF activity assays correlated well with each other and the VWF:RCo assay. The slight differences in characteristics found in the COMPASS-VWF study will assist the VWF community in interpreting and comparing activity results.

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Section: Discussionsupporting

confidence: 89%

An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: the COMPASS‐VWF study

Szederjesi

Baronciani

Budde³

et al. 2018

Journal of Thrombosis and Haemostasis

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“…Some VWD type 2M mutations (e.g. p.G1324A) are not detected by the assay and it is not clear to what extent the assay is sensitive to the loss of HMW multimers . Furthermore, the VWF:Ab assay did not resolve the problem with the lower limit of detection because linearity is reported to be acceptable only above 12.5 IU dL −1 .…”

Section: Monoclonal Antibody Binding‐based Vwf Activity (Vwf: Ab)mentioning

confidence: 98%

Platelet‐dependent von Willebrand factor activity. Nomenclature and methodology: communication from the SSC of the ISTH

Bodó

Eikenboom

Montgomery

et al. 2015

Journal of Thrombosis and Haemostasis

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128

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“…vWD is the most common inherited human bleeding disorder and results from defects in plasma vWF quantity and/or quality [49]. According to phenotype analysis, vWD can be classified among six independent types: type 1, types 2A, 2B, 2M and 2N, and type 3 [50].…”

Section: The Significance Of Vwf In Diseasesmentioning

confidence: 99%

von Willebrand Factor: More Than a Regulator of Hemostasis and Thrombosis

Luo

Yang

et al. 2012

Acta Haematol

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von Willebrand factor (vWF) was first identified as an adhesive glycoprotein involved in hemostasis by Zimmermann in 1971. Since then, vWF has been shown to play a vital role in platelet adhesion, platelet binding to collagen and factor VIII protection. Recent studies have implicated vWF as a regulator of angiogenesis, smooth muscle cell proliferation, tumor cell metastasis and crosstalk in the immune system. In this review, we will discuss the aspects of vWF structure that facilitate its biological effects and speculate on its newly discovered and hypothesized roles in the pathogenesis of several diseases.

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Evaluation of an Automated von Willebrand Factor Activity Assay in von Willebrand Disease

Cited by 24 publications

References 13 publications

An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: the COMPASS‐VWF study

An international collaborative study to compare different von Willebrand factor glycoprotein Ib binding activity assays: the COMPASS‐VWF study

Platelet‐dependent von Willebrand factor activity. Nomenclature and methodology: communication from the SSC of the ISTH

von Willebrand Factor: More Than a Regulator of Hemostasis and Thrombosis

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