Evaluation of an Automatic Calcium Titrator

Alexander, R L

doi:10.1093/clinchem/17.12.1171

Cited by 50 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Calcium. The Calcette 4009 (Precision Systems, Inc., Sudbury, MA, USA) was used to determine calcium concen¬ trations using ethylene glycol-bis-iV-JV-tetraacetic acid (EGTA) for fluorometric titration of calcium in an aqueous solution (Alexander, 1971).…”

Section: Methodsmentioning

confidence: 99%

Effects of prolactin on conceptus survival and uterine secretory activity in pigs

Young¹,

Kraeling²,

Bazer³

1989

Reproduction

View full text Add to dashboard Cite

Hypoprolactinaemia was induced by bromocriptine (CB154; 100 mg/day) which decreased circulating prolactin by 40% (P less than 0.06), but did not affect conceptus survival at Day 25 when administered on Days 10-16 when compared to saline:ethanol-treated control gilts. Bromocriptine or vehicle was administered to cyclic gilts on Days 10-11, oestradiol valerate was injected on Day 11 and uterine flushings were collected on Day 12. Total recoverable protein and uteroferrin in uterine flushings were not affected by treatment. However, leucine aminopeptidase activity (P less than 0.02) and total recoverable Ca2+, Na+, K+ and Cl- (P less than 0.05) were decreased in uterine flushings of gilts that received bromocriptine, suggesting that hypoprolactinaemia decreased general secretory activity of the endometrial epithelium and modulated ionic changes, respectively, in the uterine environment of pigs. Subcutaneous administration of pig prolactin (1 mg/12 h) increased (P less than 0.001) serum prolactin 4.5-fold. The interaction between hyperprolactinaemia and progesterone, without oestrogen, on components of uterine flushings were determined using gilts that received progesterone (200 mg/day) and prolactin or saline on Days 4-14 after ovariectomy on Day 4. On Day 15, there were no differences (P greater than 0.05) in any of the uterine secretory components measured. Hyperprolactinaemia (1 mg pig prolactin on Days 6-11) enhanced overall uterine secretory response on Day 12 to oestradiol (5 mg) administered on Day 11 compared to gilts that received 1 ml saline on Days 6-11 of the oestrous cycle. Total recoverable protein and leucine aminopeptidase activity were greater (P less than 0.05) for oestradiol-treated gilts, but effects of prolactin were not significant. Total recoverable glucose (P less than 0.01), PGF-2 alpha (P less than 0.02), uteroferrin (P less than 0.01) and specific activity of uteroferrin (P less than 0.001) were increased by prolactin and oestradiol, but not oestradiol alone. Calcium (P less than 0.05), chloride (P less than 0.05) and potassium (P less than 0.01) were increased in response to oestradiol. These results indicate an interaction between oestradiol and prolactin, but not progesterone and prolactin, which enhances secretion of some products of the pig uterine endometrium.

show abstract

Section: Methodsmentioning

confidence: 99%

Effects of prolactin on conceptus survival and uterine secretory activity in pigs

Young¹,

Kraeling²,

Bazer³

1989

Reproduction

View full text Add to dashboard Cite

show abstract

“…Calcium ion (Ca2 + ) concentrations in uterine flushings were measured with a Calcette calcium analyser (Precision Systems, Inc., Sudbury, MA, U.S.A.). The system utilized ethylene glycol tetraacetic acid for fluorometric titration of calcium in aqueous solutions (Alexander, 1971). The sensitivity of the analysis was 10"4 pg/ml.…”

Section: Methodsmentioning

confidence: 99%

Development of pig blastocysts in a uterine environment advanced by exogenous oestrogen

Morgan¹,

Geisert²,

Zavy³

et al. 1987

Reproduction

View full text Add to dashboard Cite

Routine embryo transfer techniques were used to establish recipient groups in which blastocysts were either asynchronous (blastocysts 24 h behind recipient uterus) or synchronous with their uterine environment. Oestradiol valerate (5 mg) was administered on Day 11 of the recipient's cycle to stimulate release of uterine secretion in the synchronous gilts (Group SE) and one group (AE) of asynchronous gilts. The gilts in the other asynchronous group (Group AC) were injected with vehicle (sesame oil). Embryos recovered on Day 14 by hysterectomy and flushing were evaluated for morphological development. Oestradiol treatment resulted in a failure of blastocyst development in Group AE gilts only. Recoverable oestradiol in the uterine flushings was increased in gilts in Groups AC and SE which contained elongated blastocysts. Plasmin inhibitor levels were lower in Groups AC and SE while PGF tended to be increased. Acid phosphatase activity was higher and recoverable Ca2+ was lower in Groups AE and SE. Failure of blastocyst development in Group AE is believed to have resulted from a failure to undergo trophoblastic elongation due to premature alteration of the uterine environment at a critical period of blastocyst development or from the presence of an unfavourable uterine environment for blastocyst attachment and development shortly after Day 12.

show abstract

“…Serum total calcium and urine calcium were determined by the EGTA titration method (Alexander 1971). Serum and urine creatinine and phosphate were determined by the autoanalyzer method.…”

Section: Methodsmentioning

confidence: 99%

Nephrogenous Cyclic Adenosine Monophosphate Levels in Normocalcemic Cancer Patients: Its Significance

Abramson

Kukreja²,

Lad

et al. 1984

Horm Metab Res

View full text Add to dashboard Cite

We evaluated nephrogenous cyclic adenosine monophosphate ( NcAMP ) levels in 61 normocalcemic patients with documented cancer of various organs and cell types. NcAMP levels were elevated in 17 (28%) and decreased in 13 (21%) of the cancer patients. Both high and low NcAMP levels were seen within the various cancer groups. There was a significant correlation (r = 0.383, P less than 0.01) between NcAMP and serum parathyroid hormone (PTH) levels, suggesting that tumor-related factors affecting NcAMP , may be partially related to native PTH. Alternatively, these factors might be altering the effect of endogenous PTH on renal tubules. A significant negative correlation was also observed between NcAMP and tubular maximum for phosphate (r = -0.356, P less than 0.02) suggesting that either cAMP per se or factors affecting NcAMP alter phosphate excretion. Follow up serum calcium data was available on 48 of the 61 patients. Subsequent hypercalcemia developed independent of the initial nephrogenous cAMP levels. It therefore appears that NcAMP elevation and development of hypercalcemia are two separate paraneoplastic phenomena.

show abstract

Evaluation of an Automatic Calcium Titrator

Cited by 50 publications

References 0 publications

Effects of prolactin on conceptus survival and uterine secretory activity in pigs

Effects of prolactin on conceptus survival and uterine secretory activity in pigs

Development of pig blastocysts in a uterine environment advanced by exogenous oestrogen

Nephrogenous Cyclic Adenosine Monophosphate Levels in Normocalcemic Cancer Patients: Its Significance

Contact Info

Product

Resources

About