Evaluation of an Enzyme Immunoassay for Detection of Immunoglobulin M Antibodies to West Nile Virus and the Importance of Background Subtraction in Detecting Nonspecific Reactivity

Rawlins, Mindy L.; Swenson, Erica M.; Hill, Harry R.; Litwin, Christine M.

doi:10.1128/cvi.00480-06

Cited by 16 publications

(10 citation statements)

References 21 publications

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“…9 When we began this study in 2003, we used the only nationally accepted protocol for determination of IgM and IgG antibodies at that time, which was based on the CDC recommendations and previously published studies. With the introduction of Food and Drug Administration (FDA)-approved commercially available kits through Focus Diagnostics and PanBio, Inc. a couple of years into the study, 10 we made the decision to continue to use the CDC protocol to remain consistent with methods and interpretations. In 2010, we began to use the commercially available kits by PanBio.…”

Section: Discussionmentioning

confidence: 99%

Persistence of Detectable Immunoglobulin M Antibodies Up to 8 Years After Infection with West Nile Virus

Murray¹,

Garcia²,

Yan³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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Abstract. In Houston, we have been monitoring the immune response to West Nile virus (WNV) infection in a large cohort of study participants since 2002. Using enzyme-linked immunosorbent assay techniques, serum from 163 participants was tested for the presence of anti-WNV immunoglobulin M (IgM) and IgG antibodies. We found that 42%, 34%, and 23% of study participants had either positive or equivocal results when tested for anti-WNV IgM antibodies approximately 1, 6, and 8 years post-infection, respectively. Conversely, almost one-half of study participants (46%) had undetectable anti-WNV IgG antibodies by 8 years post-infection. This study is the first study to calculate the slope of the rate of decay of antibodies over time as well as show persistence of detectable anti-WNV IgM antibodies up to 8 years post-infection. These findings warrant additional investigation, particularly the determination of whether persistence of IgM is related to persistent infection with WNV.

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Section: Discussionmentioning

confidence: 99%

Persistence of Detectable Immunoglobulin M Antibodies Up to 8 Years After Infection with West Nile Virus

Murray¹,

Garcia²,

Yan³

et al. 2013

The American Society of Tropical Medicine and Hygiene

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“…Most WNV IgM assays utilize recombinant premembrane/envelope (preM/E) protein (Davis et al, 2001). These assays exhibit excellent sensitivity for identifying individuals recently exposed to WNV (Hogrefe et al, 2004;Muerhoff et al, 2004;Holmes et al, 2005;Tilley et al, 2005;Rawlins et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Persistence of antibodies to West Nile virus nonstructural protein 5

Prince¹,

Lapé-Nixon²,

Yeh³

et al. 2008

Journal of Clinical Virology

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“…For example, despite the existence of highly sensitive tests, such as ELISA and immuno-blotting techniques, inconclusive serological results are quite frequent in infectious diseases, such as Chagas disease, tuberculosis, leprosy, and West Nile virus [74]. Recently, it was reported that serum samples from patients with myco-bacterial infections ( Mycobacterium leprae ) crossreact with HIV structural proteins Gp41, p55, and p18 [75].…”

Section: Comparison Of Elisa and Iacementioning

confidence: 99%

Immunoaffinity capillary electrophoresis as a powerful strategy for the quantification of low‐abundance biomarkers, drugs, and metabolites in biological matrices

2008

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In the last few years, there has been a greater appreciation by the scientific community of how separation science has contributed to the advancement of biomedical research. Despite past contributions in facilitating several biomedical breakthroughs, separation sciences still urgently need the development of improved methods for the separation and detection of biological and chemical substances. In particular, the challenging task of quantifying small molecules and biomolecules, found in low abundance in complex matrices (e.g., serum), is a particular area in need of new highefficiency techniques. The tandem or on-line coupling of highly selective antibody capture agents with the high-resolving power of CE is being recognized as a powerful analytical tool for the enrichment and quantification of ultra-low abundance analytes in complex matrices. This development will have a significant impact on the identification and characterization of many putative biomarkers and on biomedical research in general. Immunoaffinity CE (IACE) technology is rapidly emerging as the most promising method for the analysis of low-abundance biomarkers; its power comes from a three-step procedure: (i) bioselective adsorption and (ii) subsequent recovery of compounds from an immobilized affinity ligand followed by (iii) separation of the enriched compounds. This technology is highly suited to automation and can be engineered to as a multiplex instrument capable of routinely performing hundreds of assays per day. Furthermore, a significant enhancement in sensitivity can be achieved for the purified and enriched affinity targeted analytes. Thus, a compound that exists in a complex biological matrix at a concentration far below its LOD is easily brought to well within its range of quantification. The present review summarizes several applications of IACE, as well as a chronological description of the improvements made in the fabrication of the analyte concentrator-microreactor device leading to the development of a multidimensional biomarker analyzer.

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Evaluation of an Enzyme Immunoassay for Detection of Immunoglobulin M Antibodies to West Nile Virus and the Importance of Background Subtraction in Detecting Nonspecific Reactivity

Abstract: Since the introduction of West Nile virus (WNV) in the United

Cited by 16 publications

References 21 publications

Persistence of Detectable Immunoglobulin M Antibodies Up to 8 Years After Infection with West Nile Virus

Persistence of Detectable Immunoglobulin M Antibodies Up to 8 Years After Infection with West Nile Virus

Persistence of antibodies to West Nile virus nonstructural protein 5

Immunoaffinity capillary electrophoresis as a powerful strategy for the quantification of low‐abundance biomarkers, drugs, and metabolites in biological matrices

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