Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of sarcoptic mange in swine

Hollanders, W.; Vercruysse, Jozef; Raes, Stefaan; Bornstein, S.

doi:10.1016/s0304-4017(96)01117-x

Cited by 42 publications

(33 citation statements)

References 11 publications

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“…Antibody responses to S. scabiei infestation have been documented in domestic and wildlife hosts [1,3,8] and standardised immunosorbent assays are currently available for diagnosis and monitoring of scabies in dogs and swine [9,10,19]. To the best of our knowledge, the LAB-ELISA described here is the first immunosorbent assay designed for the detection of specific antibodies against S. scabiei in a free-ranging ruminant host.…”

Section: Discussionmentioning

confidence: 99%

Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)

et al. 2004

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-Scabies is a major threat to the well being of mountain-dwelling Bovid hosts, Rupicapra rupicapra and Rupicapra pyrenaica. Severe outbreaks are in progress over a significant part of their distribution area and resource managers demand improved methods to monitor, analyse and possibly forecast the spread and effects of scabies at the population level. An amplified capture enzyme-linked immunosorbent assay was developed to detect antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.) serum. The method used the biotin-avidin amplification system and was validated on a panel of 144 serum samples, of which 40 were obtained from scabietic and 104 from healthy unexposed individuals originating from a scabies-free area. The antigen, a whole body extract of the various developmental stages of S. scabiei, was prepared from mites actively leaving the skin lesions of naturally infested red foxes (Vulpes vulpes). The resulting LAB-ELISA was characterised by 93% sensitivity, 97% specificity and a high degree of repeatibility. A single seroreactor was found amongst 32 chamois affected with skin pathologies other than scabies, including infestations by other Acarina (Trombicula spp. and Ixodid ticks). Antibodies to S. scabiei were present in 26 out of 169 sera (15.4%) obtained by clinically healthy chamois within a scabies outbreak area, indicating that asymptomatic infestations by S. scabiei can be revealed by serological methods in the studied Caprinae hosts.Sarcoptes / chamois / ELISA / avidin-biotin

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Section: Discussionmentioning

confidence: 99%

Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)

et al. 2004

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“…The cut-off levels for the ELISA test were calculated as the mean of the relative OD 450nm from scabies-free animals plus three times the standard deviation [7,13,37].…”

Section: Elisa Testmentioning

confidence: 99%

“…In recent years, many enzyme-linked immunosorbent assays (ELISA), based on crude mite extracts have been developed for several host species [6,7,13,31,36]. S. scabiei is the source of multiple antigenic molecules [1][2][3] and the varieties of S. scabiei from different animals introduce both cross-reactive and variety specific antigens into the host [5,13]. Consequently, antigens in extracts made from one variety of scabies mite can be used to detect serum antibodies elicited by another variety [7,13,23].…”

Section: Introductionmentioning

confidence: 99%

“…It has been reported that more than 50% of skin scrapes can be negative in canine scabies infections [24]. In recent years, many enzyme-linked immunosorbent assays (ELISA), based on crude mite extracts have been developed for several host species [6,7,13,31,36]. S. scabiei is the source of multiple antigenic molecules [1][2][3] and the varieties of S. scabiei from different animals introduce both cross-reactive and variety specific antigens into the host [5,13].…”

Section: Introductionmentioning

confidence: 99%

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Identification and heterologous expression of aSarcoptes scabieicDNA encoding a structural antigen with immunodiagnostic potential

et al. 2007

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-The mite Sarcoptes scabiei causes sarcoptic mange (or scabies), a disease of considerable human and veterinary significance. An S. scabiei cDNA clone of about 2 kb was isolated from a S. scabiei var. hominis expression library by immunological screening using blood serum from a naturally infected chamois (Rupicapra rupicapra). The nucleotide sequence of the identified cDNA contains an open reading frame of 1930 bp that encodes a 642 amino acid polypeptide. This polypeptide shows tandem repeats of a glycine-serine rich 20 residue sequence followed by a unique C-terminal glutamate rich 54 residue sequence. The cDNA or the deduced polypeptide did not show significant similarities to any of the sequences in the databases. A carboxyl-terminal fragment of this polypeptide (residues 380 to 642) was efficiently expressed in Escherichia coli as a fusion with Glutathione S-transferase and then was used to produce a specific antiserum. The antigen encoded by the cDNA was located at the integument of the mite's epidermis and the cavities surrounding its vital organs. Western blot analysis of mite extracts using the specific antiserum against the recombinant protein identified antigens larger that 60 kDa indicating that the isolated cDNA did not contain the full ORF. Moreover, we designed a diagnostic assay based on the carboxyl-terminal fragment of the antigen for the identification of infected animals.Sarcoptes scabiei / cDNA library / immunolocalisation / sarcoptic mange diagnosis / ELISA

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“…The cut-off level of the ELISA test, calculated as the mean of the % relative OD 450 nm from 24 scabies-free wild rabbits plus three times the standard deviation (Bornstein and Wallgren, 1997;Hollanders et al, 1997), was estimated as 6.7%. Sera from 49 rabbits from Mallorca were also analyzed for the presence of antibodies against myxoma virus and RHD virus by an indirect ELISA (OIE, 2010).…”

Section: Serological Analysismentioning

confidence: 99%

Widespread exposure to Sarcoptes scabiei in wild European rabbits (Oryctolagus cuniculus) in Spain

Millán

Casáis

Delibes‐Mateos

et al. 2012

Veterinary Parasitology

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a b s t r a c t Sarcoptic mange was recently described in the wild European rabbit (Oryctolagus cuniculus) in north-eastern Mediterranean Spain, the first such infection reported in this species anywhere in the world. This finding has created concern in conservationists and game managers given that an outbreak of mange after a translocation would have catastrophic consequences for naïve rabbit populations in other parts of Spain. A retrospective serosurvey using an 'in house' ELISA test based on the use of a recombinant antigen aimed at determining the rates of contact with Sarcoptes scabiei was carried out on sera from 966 rabbits collected between 1993 and 2010 in Spain. Antibodies were found in 13% of wild rabbits in 60% of the 53 areas surveyed, as well as in 16 of the 17 Spanish provinces and islands studied. Seropositive rabbits were found amongst the oldest samples analyzed and in all studied years. Antibodies were also detected in 36% of rabbits from the protected island of Dragonera, where rabbits have probably not been released since the 1970s. On Mallorca, where 89 rabbits were inspected for both lesions and antibodies, the prevalence of lesions (5.6%) was much lower than the seroprevalence (22.5%), indicating that rabbits often survive infection or that ELISA detects infected rabbits before they develop visible lesions. Seroprevalence was higher in areas with medium levels of rabbit abundance, no restocking and high rainfall. The results show that mange is widespread in rabbits and that the mite is not a recent introduction. Thus, sarcoptic mange could be considered as an enzootic disease in the wild rabbit and so prophylactic measures implemented during rabbit translocations are to be encouraged to avoid local outbreaks in naïve populations.

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Evaluation of an enzyme-linked immunosorbent assay (ELISA) for the serological diagnosis of sarcoptic mange in swine

Cited by 42 publications

References 11 publications

Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)

Biotin-avidin amplified ELISA for detection of antibodies to Sarcoptes scabiei in chamois (Rupicapra spp.)

Identification and heterologous expression of aSarcoptes scabieicDNA encoding a structural antigen with immunodiagnostic potential

Widespread exposure to Sarcoptes scabiei in wild European rabbits (Oryctolagus cuniculus) in Spain

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