Evaluation of an extended set of 15 candidate STR loci for paternity and kinship analysis in an Austrian population sample

Grubwieser, Petra; Zimmermann, Bettina; Niederstätter, Harald; Pavlic, Marion; Steinlechner, Martin; Parson, Walther

doi:10.1007/s00414-006-0079-9

Cited by 31 publications

(21 citation statements)

References 35 publications

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“…In the field of forensic DNA analysis including identification, stain analysis and kinship testing, the investigation of short tandem repeat (STR) markers of nuclear DNA has proven to be a valuable and sensitive method [13,15,18].…”

Section: Introductionmentioning

confidence: 99%

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

et al. 2006

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Low volume (LV) amplification (1 μL) of nuclear DNA (nucDNA) on a chemically structured chip is an appropriate and highly sensitive method to simultaneously amplify amelogenin and 15 forensically relevant short tandem repeats (STR). In this study, a combined method using on-chip LV amplification of mitochondrial DNA (mtDNA) and subsequent on-chip LV cycle sequencing was established to obtain a method, which is sensitive and robust enough to allow reliable analysis of DNA amounts representing the single cell level. All the necessary steps of the procedure-except for the purification of the sequencing products-were accomplished within the same final 2-μL reaction volume.

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Section: Introductionmentioning

confidence: 99%

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

et al. 2006

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“…Even "normal" cases with child, mother and putative father can become "difficult" when mutations are encountered. In these cases even typing multiple brands of the commercial kits does not provide the desired discriminatory power, as the majority of the loci are shared [4]. Another problem is that most STRs in commercially available kits are tailor-made for forensic stain analysis rather than for paternity testing, as evidenced by their high sensitivity and relatively low polymorphism.…”

Section: Introductionmentioning

confidence: 90%

“Paterniplex”, a highly discriminative decaplex STR multiplex tailored for investigating special problems in paternity testing

et al. 2007

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The goal of the study was to develop a STR multiplex ("Paterniplex") that is--as supplement to commercially available multiplex kits like the Identifiler kit (Applied Biosystems, Foster City, CA)--suitable for solving complex paternity cases such as deficiency cases or cases with mutations. The Paterniplex comprises the nine highly polymorphic STRs D8S1132, D7S1517, D10S2325, D12S391, Se33, D17S976, Penta E, Penta D and FGA in addition to Amelogenin as sex determination marker. The loci were selected because of their high degree of polymorphism (higher than that of the widely used TH01 marker). Only one locus, FGA, is shared with the Identifiler kit to avoid sample mix up. The study further gives details on the population genetics of the loci in a German Caucasian population (allelic distribution, Hardy-Weinberg Equilibrium and forensic efficiency markers such as the Discriminating Power) and three examples for cases that could not be solved using commercially available kits alone, but using the Paterniplex in addition to a commercial kit.

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“…Zygotic studies on lymphocytes and buccal cells of the twins by polymerase chain reaction (PCR) by using 4 microsatellite markers (D10S2325, D14S608, amelogenin, and D8S1179) were performed as previously described. 6 Preliminary analyses involving 17 polymorphic microsatellite markers on the parental DNA by PCR revealed these 4 markers that can be used for the purpose of judging zygosity. The microsatellite analysis on blood lymphocytes of the twins demonstrated an admixture of microsatellite types from both twins.…”

Section: Patient Presentationmentioning

confidence: 99%

Chimerism of Buccal Membrane Cells in a Monochorionic Dizygotic Twin

Fumoto¹,

Hosoi²,

Ohnìshì

et al. 2014

Pediatrics

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No monochorionic dizygotic twins (MCDZTs) with cellular chimerism involving cells other than blood cells have been reported in the literature to date. Here we report a probable first case of MCDZTs with buccal cell chimerism. A 32-year-old woman conceived twins by in vitro fertilization by using 2 cryopreserved blastocysts that were transferred into her uterus. An ultrasound scan at 8 weeks’ gestation showed signs indicative of monochorionic twins. A healthy boy and a healthy girl were born, showing no sexual ambiguity. Cytogenetic analyses and microsatellite studies demonstrated chimerism in blood cells of both twins. Notably, repeated fluorescence in situ hybridization and microsatellite studies revealed chimerism in buccal cells obtained from 1 of the twins. Although the mechanism through which buccal cell chimerism was generated remains to be elucidated, ectopic differentiation of chimeric hematopoietic cells that migrated to the buccal membrane or the cellular transfer between the 2 embryos at the early stage of development might be responsible for the phenomenon. This hypothesis raises an interesting issue regarding embryonic development and cellular differentiation into organs during fetal development. Given the possibility of cryptic chimerism in various organs including gonadal tissues in MCDZTs, close observation will be required to determine whether complications develop in the course of the patients’ growth.

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Evaluation of an extended set of 15 candidate STR loci for paternity and kinship analysis in an Austrian population sample

Cited by 31 publications

References 35 publications

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip

“Paterniplex”, a highly discriminative decaplex STR multiplex tailored for investigating special problems in paternity testing

Chimerism of Buccal Membrane Cells in a Monochorionic Dizygotic Twin

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