Evaluation of an extract of North American ginseng (Panax quinquefolius L.) in Candida albicans-infected complement-deficient mice

Trammell, Rita A; Cox, Lisa; Pikora, Joshua; Murphy, Laura; Toth, Linda A

doi:10.1016/j.jep.2011.11.026

Cited by 12 publications

(7 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heart, Brain and both kidneys were recovered for C. albicans culture. The efficacy of the Panax quinquefolius extract was observed by the higher survival of animals and reduction im CFU counting, mainly in the kidneys (Trammell et al, 2012 ). Therefore, our results strongly suggest that E. uniflora impairs proper hypha formation in C. albicans and this phenomenon may reflect directly in pathogenesis which may be observed in the decrease of CFU counting in yeast treated cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Ethyl Acetate Fraction of Eugenia uniflora on Proteins Global Expression during Morphogenesis in Candida albicans

et al. 2017

View full text Add to dashboard Cite

Candida albicans is able to switch from yeast to hyphal growth and this is an essential step for tissue invasion and establishment of infection. Due to the limited drug arsenal used to treat fungal infections and the constant emergence of resistant strains, it is important to search for new therapeutic candidates. Therefore, this study aimed to investigate by proteomic analysis the role of a natural product (Eugenia uniflora) in impairing hypha formation in C. albicans. We also tested the potential action of E. uniflora to prevent and treat oral candidiasis induced in a murine model of oral infection and the ability of polymorphonuclear neutrophils to phagocytize C. albicans cells treated with the ethyl acetate fraction of the extract. We found that this fraction greatly reduced hypha formation after morphogenesis induction in the presence of serum. Besides, several proteins were differentially expressed in cells treated with the fraction. Surprisingly, the ethyl acetate fraction significantly reduced phagocytosis in C. albicans (Mean 120.36 ± 36.71 yeasts/100 PMNs vs. 44.68 ± 19.84 yeasts/100 PMNs). Oral candidiasis was attenuated when C. albicans cells were either pre-incubated in the presence of E. uniflora or when the fraction was applied to the surface of the oral cavity after infection. These results were consistent with the reduction in CFU counts (2.36 vs. 1.85 Log10 CFU/ml) and attenuation of tissue damage observed with histopathological analysis of animals belonging to treated group. We also observed shorter true hyphae by direct examination and histopathological analysis, when cells were treated with the referred natural product. The E. uniflora ethyl acetate fraction was non-toxic to human cells. E. uniflora may act on essential proteins mainly related to cellular structure, reducing the capacity of filamentation and attenuating infection in a murine model, without causing any toxic effect on human cells, suggesting that it may be a future therapeutic alternative for the treatment of Candida infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of the Ethyl Acetate Fraction of Eugenia uniflora on Proteins Global Expression during Morphogenesis in Candida albicans

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Quinqueginsing, a protein purified from American ginseng roots, suppressed fungal growth against C. comatus (Wang & Ng, ). Although ingestion of hot water extract of American ginseng roots, which includes both ginsenosides and soluble polysaccharides, was associated with significantly lower C. albicans titers in the kidney of C. albicans ‐infected mice (DBA/2J) than the control non‐treatment infected group (Trammell, Cox, Pikora, Murphy, & Toth, ). Besides, pure ginsenoside Rb2, Rd, and Re (Figure ) were evidenced to have antifungal activity against C. albicans 533 with MIC values of <1 to 9 μg/ml (Battinelli et al, ).…”

Section: Antimicrobial Propertiesmentioning

confidence: 99%

Antimicrobial activities of Asian ginseng, American ginseng, and notoginseng

Wang

Huang

Yin

et al. 2019

Phytotherapy Research

View full text Add to dashboard Cite

Asian ginseng (Panax ginseng C.A. Meyer), American ginseng (Panax quinquefolius) and notoginseng (Panax notoginseng) are the three most commonly used ginseng botanicals in the world. With the increasing interests on antimicrobial properties of plants, the antimicrobial activities of ginseng species have been investigated by a number of researchers worldwide. This overview interprets our present knowledge of the antimicrobial activities of the three ginseng species and some of their bioactive components against pathogenic bacteria (Pseudomonas aeruginosa, Helicobacter pylori, Staphylococcus aureus, Escherichia coli, Propionibacterium acnes, et al.) and fungi (Candida albicans, Fusarium oxysporum, et al). Ginsenosides, polysaccharides, essential oil, proteins, and panaxytriol are all might responsible for the antimicrobial activities of ginseng. The antimicrobial mechanisms of ginseng components could be summarized to the following points: (a) inhibit the microbial motility and quorum-sensing ability; (b) affect the formation of biofilms and destroy the mature biofilms, which can weaken the infection ability of the microbes; (c) perturb membrane lipid bilayers, thus causing the formation of pores, leakages of cell constituents and eventually cell death; (d) stimulate of the immune system and attenuate microbes induced apoptosis, inflammation, and DNA damages, which can protect or help the host fight against microbial infections; and (e) inhibit the efflux of antibiotics that can descend the drug resistance of the microbial. The collected information might facilitate and guide further studies needed to optimize the use of ginseng and their components to improve microbial food safety and prevent or treat animal and human infections.

show abstract

“…Yeasts were then washed and prepared in PBS at a concentration of 3 ϫ 10 8 /ml for inoculation. Mice were anesthetized with isoflurane and injected intravenously via the retro-orbital sinus (29) with 100 l of yeast suspension or PBS alone as a negative control. All mice were evaluated on a daily basis for 21 days.…”

Section: Methodsmentioning

confidence: 99%

Host-Pathogen Interaction and Signaling Molecule Secretion Are Modified in thedpp3Knockout Mutant of Candida lusitaniae

et al. 2014

View full text Add to dashboard Cite

e Candida lusitaniae is an emerging opportunistic yeast and an attractive model to discover new virulence factors in Candida species by reverse genetics. Our goal was to create a dpp3⌬ knockout mutant and to characterize the effects of this gene inactivation on yeast in vitro and in vivo interaction with the host. The secretion of two signaling molecules in Candida species, phenethyl alcohol (PEA) and tyrosol, but not of farnesol was surprisingly altered in the dpp3⌬ knockout mutant. NO and reactive oxygen species (ROS) production as well as tumor necrosis factor alpha (TNF-␣) and interleukin 10 (IL-10) secretion were also modified in macrophages infected with this mutant. Interestingly, we found that the wild-type (WT) strain induced an increase in IL-10 secretion by zymosan-activated macrophages without the need for physical contact, whereas the dpp3⌬ knockout mutant lost this ability. We further showed a striking role of PEA and tyrosol in this modulation. Last, the DPP3 gene was found to be an essential contributor to virulence in mice models, leading to an increase in TNF-␣ secretion and brain colonization. Although reinsertion of a WT DPP3 copy in the dpp3⌬ knockout mutant was not sufficient to restore the WT phenotypes in vitro, it allowed a restoration of those observed in vivo. These data support the hypothesis that some of the phenotypes observed following DPP3 gene inactivation may be directly dependent on DPP3, while others may be the indirect consequence of another genetic modification that systematically arises when the DPP3 gene is inactivated.

show abstract

Evaluation of an extract of North American ginseng (Panax quinquefolius L.) in Candida albicans-infected complement-deficient mice

Cited by 12 publications

References 48 publications

Effect of the Ethyl Acetate Fraction of Eugenia uniflora on Proteins Global Expression during Morphogenesis in Candida albicans

Effect of the Ethyl Acetate Fraction of Eugenia uniflora on Proteins Global Expression during Morphogenesis in Candida albicans

Antimicrobial activities of Asian ginseng, American ginseng, and notoginseng

Host-Pathogen Interaction and Signaling Molecule Secretion Are Modified in thedpp3Knockout Mutant of Candida lusitaniae

Contact Info

Product

Resources

About