Evaluation of an extract of the Punica granatum flower as a biological stain of rat tissues: a preliminary study

Kuşçulu, Nilgün Güler; Aslan, Halime Güzin

doi:10.1007/s11033-018-4510-3

Cited by 3 publications

(5 citation statements)

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“…In another study, light and pale red staining occurred in the liver, kidney, heart, and lung tissues of Wistar albino rats with an aqueous extract without mordant of Hibiscus sabdariffa in the cytoplasm. 3 Thus, in this study, it was decided to apply the Hibiscus sabdariffa on the same tissues as a cytoplasm dye. Water, alcohol and chloroform extracts of basil, alkanet and red cabbage were used to stain the nucleus in hematoxylin color.…”

Section: Discussionmentioning

confidence: 99%

“…The rat was put to euthanized with ketamine + xylazine and its tissues were taken. All of the procedures were performed according to ethical guidelines [3].…”

Section: Tissue Supplymentioning

confidence: 99%

“…The remaining blue-purple colored ltrate was used as the dye solution. The ltrate was stored in the refrigerator at 4°C and then was used for staining [12,3].…”

Section: Preparation Of Red Cabbage Leaves Extractmentioning

confidence: 99%

“…The intensity of staining was evaluated as negative (−), weak (+), medium (++), and very intense (+++). 3 The best staining intensity (+++) in the evaluation of the staining result was considered as H&E stained tissue.…”

Section: Preparation Of the Tissue Preparatesmentioning

confidence: 99%

“…It is known that natural dyes have been used for coloring Textil materials [2]. However, there are also studies with natural dyes on the staining of tissues, which are a different discipline [3]. Biopsy specimens or tissue fragments are taken from animals or humans in medical and research laboratories.…”

Section: Introductionmentioning

confidence: 99%

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Dual Staining Effect of Extracts Roselle (Hibiscus Sabdariffa L.) and Red Cabbage (Brassica Oleracea) on Wistar Rat Tissues

Kuşçulu

2021

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Background: Apart from the areas where natural dyes are used a lot, if staining studies on tissues increase, their use in pathology laboratory will become widespread. Two basic elements in cell tissues, the nucleus, and cytoplasm, should be stained and evaluated together for histopathological diagnosis. The most commonly used dye combinations for this diagnosis are Hematoxylin-Eosin dyes, also known as nuclei and cytoplasm dyes. Histological staining studies of natural origin did not reveal staining studies on the same tissue with the extracts of two plants. Methods and Results: In this study, roselle (Hibiscus sabdariffa L.) and red cabbage (Brassica oleracea) leaves were used as a source of natural dye and also liver and kidney tissues of Wistar albino rat as dye biomaterials. The nuclei and cytoplasm regions of rat kidney and liver tissues were stained in different colors with these plants and the use of metal salts. Relying upon hematoxylin-eosin should it ever be scarce will result in the scarcity of plant-based dyes on the market.Conclusion: Since a more colorful, different nucleus and cytoplasm staining was obtained compared to previous work with the same tissues. It is expected that the introduction of new natural dye alternatives where histological dye production is not widespread will contribute to the world’s economy and industry.

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Section: Discussionmentioning

confidence: 99%

“…The rat was put to euthanized with ketamine + xylazine and its tissues were taken. All of the procedures were performed according to ethical guidelines [3].…”

Section: Tissue Supplymentioning

confidence: 99%

“…The remaining blue-purple colored ltrate was used as the dye solution. The ltrate was stored in the refrigerator at 4°C and then was used for staining [12,3].…”

Section: Preparation Of Red Cabbage Leaves Extractmentioning

confidence: 99%

Section: Preparation Of the Tissue Preparatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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